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1 KnockOut™ Serum Replacement - Thermo Fisher Scientific
https://www.thermofisher.com/order/catalog/product/10828028
KnockOut SR is a defined, serum replacement used to support the growth of undifferentiated embryonic stem (ES), human and mouse induced pluripotent stem ...
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2 Composition of Knockout Serum Replacement
https://plos.figshare.com/articles/dataset/_Composition_of_Knockout_Serum_Replacement_/611010
Composition of Knockout Serum Replacement. ... 1, KOSR ingredients. 2, Amino Acids, Glycine, L-histidine, L-isoleucine, L-methionine, ...
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3 What is the composition of Knockout Serum Replacement ...
https://www.researchgate.net/post/What_is_the_composition_of_Knockout_Serum_Replacement_KSR
I am differentiating skeletal muscle and the protocol I am using calls for KSR to be added as part of my DK-I, DK-HI, and DK-HIFL media to be used to make ...
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4 Serum Replacement - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/engineering/serum-replacement
Knockout Serum Replacer (Price et al., 2002) is a proprietary serum replacement consisting of amino acids, vitamins, transferrin or substitutes, insulin or ...
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5 KOSR - Wikipedia
https://en.wikipedia.org/wiki/KOSR
Within molecular and cell biology, KOSR, knockout serum replacement, is a defined serum-free formulation optimized to grow and maintain undifferentiated ...
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6 [Knockout serum replacement improves establishment ...
https://pubmed.ncbi.nlm.nih.gov/17343798/
Aim: To eliminate the influence of serum on self-renewal of embryonic stem cells (ESCs), knockout serum replacement (KSR), a defined formulation, ...
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7 Comparative Analysis of KnockOut™ Serum with Fetal Bovine ...
https://www.hindawi.com/journals/joph/2016/7304812/
KnockOut serum replacement (SR) is a defined serum-free formulation, which was designed to directly replace FBS for maintenance of embryonic stem cells (ESCs) ...
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8 KSR - Multi-Species: Feeder-based PSC culture proven more ...
https://www.youtube.com/watch?v=IwxvDPX8wsg
Thermo Fisher Scientific
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9 Establishment of Alternative Culture Method for ... - PLOS
https://journals.plos.org/plosone/article/comment?id=10.1371/annotation/6147394f-e169-45e9-92a5-7cc4425ca55a
We would like to supplement our statement regarding that Knockout Serum Replacement (KSR) can substitute bovine serum albumin (BSA) for in ...
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10 Knockout serum replacement (KSR) has a suppressive effect ...
https://go.gale.com/ps/i.do?id=GALE%7CA186903305&sid=googleScholar&v=2.1&it=r&linkaccess=abs&issn=15362302&p=AONE&sw=w
Cynomolgus ES cells transduced with a SeV vector expressing the green fluorescent protein (GFP) gene were propagated in Knockout serum replacement (KSR)- ...
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11 Conditioned Media for Embryonic & Induced Pluripotent Stem ...
https://www.rndsystems.com/products/conditioned-media-embryonic-induced-pluripotent-stem-cell-culture
Serum-free media (80% Knockout™ D-MEM, 20% Knockout serum replacement, ... Knockout, GlutaMAX, and KSR are trademarks of Life Technologies, Carlsbad, CA.
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12 A potential role of knockout serum replacement as a porcine ...
https://onlinelibrary.wiley.com/doi/10.1002/jcp.26489
KSR is composed of small organic molecules, trace elements, and three proteins, namely insulin, transferrin, and lipid-rich albumin, without any ...
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13 Serum free and speciality media
https://www.fishersci.co.uk/webfiles/uk/web-docs/UKLS_0222.PDF
Knockout™ serum replacement (Knockout™ SR) is a defined, serum-free formulation ... Currently, Invitrogen's KnockOut™ SR (KSR) formulation is used in ...
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14 Supplementation of culture medium with knockout serum ...
https://www.cambridge.org/core/journals/zygote/article/supplementation-of-culture-medium-with-knockout-serum-replacement-improves-the-survival-of-bovine-secondary-follicles-when-compared-with-other-protein-sources-during-in-vitro-culture/26BEAAF3D849AE43A93C51ACD81F6211
The present study evaluated the effect of knockout serum replacement (KSR), fetal bovine serum (FBS) and bovine serum albumin (BSA) on the ...
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15 Knockout Serum Replacement as a Substitute for Exosome ...
https://www.transresurology.com/article_121000_0d97cd51e8bd972be9b7d51ed7f01c94.pdf
KSR is an exosome-free serum since it has a defined formulation and stable concentration of ingredients. In summary, using an exosome-free serum ...
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16 Generating a Cost‐Effective, Weekend‐Free Chemically ...
https://labs.feinberg.northwestern.edu/burridge/docs/fonoudicpsc2020.pdf
formula for weekend-free culture of human induced pluripotent stem cells ... layer and medium containing knockout serum replacement (KSR) result in variable ...
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17 Abbreviation / Long Form : KSR / knockout serum replacement
http://allie.dbcls.jp/pair/KSR;knockout+serum+replacement.html
› pair › KSR
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18 Serum-free culture system for spontaneous human ... - bioRxiv
https://www.biorxiv.org/content/10.1101/666313v2.full
1 B-D and Fig. S1). KSR is a substitution of FBS and all the components are well defined, consisting of albumin, transferrin, insulin, collagen ...
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19 hES-maintenance-v3.pdf - Stem Cell COREdinates
http://www.coredinates.org/resources/hES-maintenance-v3.pdf
[MEF preparation]. 1) The day before passage, ... eliminate traces of serum.Aspirate PBS and add fresh hESC ... 200 ml Knockout serum replacement (KSR).
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20 Accelerated and Improved Differentiation of Retinal Organoids ...
https://www.cell.com/cms/10.1016/j.stemcr.2017.11.001/attachment/df4da5d6-58a6-42b7-ad16-28fa390d7079/mmc1
GMEM: Glasgow minimum essential medium; KSR: knockout serum replacement; RMM: retinal maturation medium constituted of DMEM/Fl2 with GlutaMAX, ...
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21 KnockOut™ Serum Replacement - Multi-Species: Feeder ...
https://www.youtube.com/watch?v=CGYn-yqRUq8
Thermo Fisher Scientific
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22 Effects of Knockout Serum Replacement in the Culture ...
https://www.koreascience.or.kr/article/JAKO201019451500063.pdf
10% knockout serum replacement (KSR) and 20% KSR in the medium. ... as these formulations typically resulted in improved construct.
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23 Cryopreservation of calf testicular tissues with ... - Europe PMC
https://europepmc.org/article/med/31954710
... from cryopreserved testicular tissues, the cryoprotection effects of the freezing media containing knockout serum replacement (KSR) were examined.
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24 Culture of Porcine Embryonic Germ Cells in Serum ...
https://www.liebertpub.com/doi/10.1089/clo.2007.0085
The purpose of this study was to determine if FBS can be replaced with Knockout Serum Replacement™ (KSR), a defined medium supplement, ...
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25 US20130059377A1 - Stem cell defined media for xeno-free ...
https://www.google.com/patents/US20130059377
6 : H-9s cultured at media with KSR and one the central formulation from RCCD. ... to replace fetal bovine serum (FBS) and Knockout Serum Replacement (KSR) ...
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26 Cryopreservation of calf testicular tissues with knockout serum ...
https://agris.fao.org/agris-search/search.do?recordID=US202100071656
... the freezing media containing knockout serum replacement (KSR) were examined. ... which is useful in future study due to its defined formulation that is ...
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27 [Knockout serum replacement improves establishment efficiency of ...
https://www.semanticscholar.org/paper/%5BKnockout-serum-replacement-improves-establishment-Wang-Tian/9266d0718e28269eba1bc2e90e51c741344d8500
AIM To eliminate the influence of serum on self-renewal of embryonic stem cells (ESCs), knockout serum replacement (KSR), a defined formulation, ...
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28 Non-Animal Cell Culture Products and Applications
https://www.thepsci.eu/wp-content/uploads/2016/11/2016.11-FBS-free-medium-table-and-information.pdf
Animal Free. Stem Cells. KnockOut™ DMEM supplemented with 15% KnockOut™ Serum. Replacement XenoFree (KSR XF) ...
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29 McLean-2007-Ly-paper.pdf - ViaCyte
https://viacyte.com/wp-content/uploads/2019/10/McLean-2007-Ly-paper.pdf
tivin A and low fetal calf serum (FCS)/knockout serum replace- ment (KSR). Reduced insulin/insulin-like growth factor (IGF).
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30 敲除血清替代品可提高C57BL / 6J小鼠胚胎干细胞系的建立效率。
https://www.x-mol.com/paper/1212993959919554564?adv
AIM To eliminate the influence of serum on self-renewal of embryonic stem cells (ESCs), knockout serum replacement (KSR), a defined formulation, ...
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31 Differentiation of iPSC into dopaminergic neurons - Protocols.io
https://www.protocols.io/view/differentiation-of-ipsc-into-dopaminergic-neurons-dm6gprq88vzp/v1
KSR (knockout serum replacement) medium composition and storage: Mix 409.5 mL of knockout DMEM/F-12 medium with 75 mL knockout serum replacement (15%), ...
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32 Differentiation of Intrafusal Fibers from Human Induced ...
https://pubs.acs.org/doi/abs/10.1021/acschemneuro.0c00055
On the sixth day, cultures were switched to medium containing knockout serum replacement (KSR),. hepatocyte growth factor (HGF), ...
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33 Heparin promotes the growth of human embryonic stem cells ...
https://www.pnas.org/doi/10.1073/pnas.0806136105
However, the precise formulation of KSR is not in the public domain and, ... (Invitrogen) supplemented with Knockout Serum Replacement (KSR, ...
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34 Cryopreserving and Recovering of Human iPS Cells using ... - JoVE
https://www.jove.com/v/2237?language=Dutch
... iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium ... in complete KnockOut SR Feeder Free (KSR-FF) medium or feeder-based KnockOut ...
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35 M6 reprogramming medium preparation - Bio-protocol
https://bio-protocol.org/bio101/r9768644
The basal medium contained knockout DMEM (Gibco), 10% KSR (KnockOut serum replacement) (Gibco), 10% FBS (Gibco), 1% NEAA (Gibco), ...
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36 Effects of different culture systems on the culture of prepuberal ...
https://vetsci.org/pdf/10.4142/jvs.2020.21.e13
with an unknown composition (18.31 ± 0.88 and 16.67 ± 1.33, respectively) (Fig. 3I). ... SSC, spermatogonia stem cell; KSR, KnockOut Serum Replacement; FBS, ...
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37 EP003812454A1* - EP 3 812 454 A1 - European Patent Office
https://data.epo.org/publication-server/rest/v1.0/publication-dates/20210428/patents/EP3812454NWA1/document.pdf
MEDIUM COMPOSITION FOR CULTURING PORCINE PLURIPOTENT STEM CELLS ... includes: 20% (v/v) of Knockout™ Serum Replacement (KSR; Gibco); 0.1% ...
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38 Preparation of mouse embryonic fibroblast cells suitable for culturing ...
https://pure.mpg.de/pubman/item/item_1719417_4
Next, FBS was replaced with knockout serum replacement (KSR) and FGF2, which enhances proliferation of hESCs(3). Finally, feeder-free culture systems enable ...
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39 Comparison of a xeno-free and serum-free culture system for ...
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-016-0347-7
Preparation of xeno-free human foreskin fibroblast cells (XF-HFF) ... with 20 % KnockOut Serum Replacement (KSR medium) (XC-HFF/KSR group).
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40 High-throughput, real-time monitoring of engineered skeletal ...
https://journals.sagepub.com/doi/pdf/10.1177/20417314221122127
replaced with 15% knockout serum replacement (KSR;. Thermo Fisher Scientific), 2 ng/mL IGF-1 ... was again supplemented into medium and this composition.
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41 Culturing hPSCs - From FBS to a Completely Defined Culture ...
https://cellculturedish.com/culturing-hpscs-from-fbs-to-a-completely-defined-culture-system/
To begin with, FBS was replaced with a serum-free commercial product called Knockout™ Serum Replacement (KSR) by Life Technologies™ (Amit et ...
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42 Monitoring Differentiation of Embryonic Stem Cells by ...
https://www.beckman.com/gated-media?mediaId=%7B0B6D9A73-348C-40BF-B8CC-9F9DDCE2D7F9%7D
by Automated Flow Cytometry Sample Preparation on the Biomek NXP Laboratory Automation Workstation ... knockout serum replacement (KSR), non essential amino.
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43 Feeder-Free Culture & Passaging of Human iPS Cells Using ...
http://www.ulab360.com/files/prod/manuals/201511/18/10064002.html
Knockout™ DMEM supplemented with KnockOut™ Serum Replacement is the media of ... using complete KnockOut™ Serum Replacement XenoFree Feeder-Free medium (KSR ...
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44 Knockout Serum Replacement (Thermo Fisher) - Bioz
https://www.bioz.com/result/knockout%20serum%20replacement/product/Thermo%20Fisher
DMEM/F12, i: ITS, C: CHIR-99021, L: LDN-193189, F: FGF-2, K: KSR, H: HGF, and I: IGF-1. The detailed composition of the medium was described in ref. 19 . ( B ) ...
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45 A simple improvement of the conventional cryopreservation for ...
https://protocols.scienceexchange.com/protocols/a-simple-improvement-of-the-conventional-cryopreservation-for-human-es-and-ips-cells
11965-092); Knockout serum replacement (KSR, Invtirogen, cat. no. ... Preparation of feeder seeded culture flasks before thawing of hES/hiPS ...
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46 Serum replacement with a growth factor–free synthetic ...
https://www.fertstert.org/article/S0015-0282(06)01078-8/abstract
that the replacement of fetal bovine serum (FBS) with a synthetic substance of knockout serum replacement (KSR) in ES cell culture medium ...
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47 Strategies for directing the differentiation of stem cells into the ...
https://academic.oup.com/cardiovascres/article/62/1/34/372066
Most notable of these is Knockout serum replacement (KSR), which was specifically developed for the maintenance of ES cells in an undifferentiated state ...
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48 An Efficient Protocol for Single-Cell Cloning Human ... - Frontiers
https://www.frontiersin.org/articles/10.3389/fcell.2019.00011/full
Furthermore, serum-free, defined media formulations were also ... KnockOut Serum Replacement (KSR; Thermo Fisher Scientific, 10828028).
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49 Generation of functional heart organoids from mouse ...
https://www.researchsquare.com/article/pex-1078/v1.pdf
Knockout Serum Replacement (KSR) (Gibco, 10828-028) ... Heart organoid medium: 20% KSR, 1 mM sodium pyruvate, 100 μM β-mercaptoethanol, ...
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50 Induced Pluripotent Stem Cell Protocols, including skin biopsy ...
https://www.alz.washington.edu/BIO/FINAL_iPSC_BestPractices_04_16_2014.pdf
KSR is thawed at 4°C, aliquoted into 100ml conical tubes and frozen at –20°C. ... include knockout serum replacement media with 10μM TGF-b inhibitor.
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51 KnockOut Serum Replacement from Thermo Fisher Scientific
https://www.biocompare.com/14966-Human-Embryonic-Stem-Cell-Media/66545-GIBCO-Knockout-Serum-Replacement/
Gibco KnockOut Serum Replacement (KnockOut SR) is a more defined, ... FBS-free formulation Enjoy the peace of mind of having a more stable price and supply ...
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52 Generation of hematopoietic stem cells from human ...
https://www.bloodresearch.or.kr/journal/view.html?uid=2033&vmd=Full
When the cells were 70% confluent, we initiated HSC differentiation by three methods involving (1) knockout serum replacement (KSR), cytokines, TGFb1, EPO, ...
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53 ERX2999595 - ChIP-Atlas
http://chip-atlas.org/view?id=ERX2999595
For adherent cells, mouse salivary gland tumor cells were cultured in DMEM/F12 medium supplemented with 20% knockout serum replacement (KSR), non-essential ...
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54 [PDF] Preparation of Mouse Embryonic Fibroblast Cells Suitable for ...
https://www.scinapse.io/papers/2013127588
... serum (FBS)-containing culture medium2. Next, FBS was replaced with knockout serum replacement (KSR) and FGF2, which enhances proliferation of hESCs3.
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55 A Comparative Analysis of Human Embryonic Stem Cells ...
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/875/020/an1237en00.pdf
The medium has a proprietary formulation that uses only humanized or synthetic components, ... Knockout™ Serum Replacement (KOSR) medium conditions.
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56 有没有人知道KSR(knockout serum replacement )的成分?
http://www.stemcell8.cn/thread-18438-1-1.html
Translate this page
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57 E-MTAB-7514 < ArrayExpress < BioStudies < EMBL-EBI
https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-7514/protocols/
For mRNA-seq, mRNA was extracted according to the standard TRIzol protocol (Invitrogen) and subjected to library preparation using the TruSeq stranded mRNA ...
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58 WO1998030679A1 - Embryonic stem cell serum replacement
https://patents.google.com/patent/WO1998030679A1/en
The present invention provides a serum-free supplement which supports the growth of embryonic stem cells in culture. Also provided are a medium comprising a ...
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59 0회 - 한국학술지인용색인
https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001432615
Effects of Knockout Serum Replacement in the Culture Medium on the ... 10% fetal bovine serum (FBS, positive control),10% knockout serum replacement (KSR) ...
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60 User Guide: KnockOut SR
http://www.ujmoreway.com/Images/Upload/file/20150529/20150529110514_1453.pdf
Description. KnockOut™ Serum Replacement (KnockOut™ SR) is a defined, serum-free formulation designed to directly replace fetal bovine serum (FBS).
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61 Cryopreservation of calf testicular tissues with knockout serum ...
https://ur.booksc.eu/book/80949467/01715f
Cryopreservation of calf testicular tissues with knockout serum replacement | Jiang, Yu; Zhu, Wen-Qian; Zhu, Xian-Chun; Cai, Ning-Ning; Yang, Rui; Cai, ...
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62 n2b27 medium composition - Regulation Partners
https://regulationpartners.com/cpsvz/n2b27-medium-composition
Fresh medium was applied without Y-27632. tablished for the mouse [N2B27 medium with 1% knockout serum replacement (KSR), activin, and basic fibroblast ...
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63 Citrates—Advances in Research and Application: 2013 Edition: ...
https://books.google.com/books?id=Mb795aaJ5zMC&pg=PA52&lpg=PA52&dq=ksr+knockout+serum+replacement+formulation&source=bl&ots=5PC0ZrhSRT&sig=ACfU3U0AzNvHsoZeQezhK_Pl7IQMemHiTA&hl=en&sa=X&ved=2ahUKEwiW74PB3bj7AhU3qZUCHcdCBUIQ6AF6BQjdARAD
... serum-substitutes, e.g., Knockout Serum Replacement (KSR) (Invitrogen), ... Moreover, its formulation is not fully known (GARCIA-GONZALO, 2008).
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64 Induced Pluripotent Stem Cells and Human Disease: Methods ...
https://books.google.com/books?id=cKd4EAAAQBAJ&pg=PA337&lpg=PA337&dq=ksr+knockout+serum+replacement+formulation&source=bl&ots=KZyAzPZO15&sig=ACfU3U1xWdVHZQcXMp5hImws5Owc8Qr_HA&hl=en&sa=X&ved=2ahUKEwiW74PB3bj7AhU3qZUCHcdCBUIQ6AF6BQjrARAD
2.1 Preparation of Matrigel-Coated Plates Thaw a bottle of Matrigel at 4 C ... CM Passaging Media: Add 10 mL of knockout serum replacement (KSR) to 90 mL of ...
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65 Cardiac Regeneration - Page 80 - Google Books Result
https://books.google.com/books?id=vrc7DwAAQBAJ&pg=PA80&lpg=PA80&dq=ksr+knockout+serum+replacement+formulation&source=bl&ots=dzjpH1eDBr&sig=ACfU3U39k-4_xmz1-Z7GNv8iGU3drn6EFw&hl=en&sa=X&ved=2ahUKEwiW74PB3bj7AhU3qZUCHcdCBUIQ6AF6BQjsARAD
Red text indicates the name of the media formulation when commercialized. ... serum albumin, KO-DMEM, KnockOut DMEM, KSR KnockOut serum replacement (Xu et ...
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66 Human Stem Cell Manual: A Laboratory Guide
https://books.google.com/books?id=y0Kowm1ltlIC&pg=PA15&lpg=PA15&dq=ksr+knockout+serum+replacement+formulation&source=bl&ots=5B6aTGZgBB&sig=ACfU3U2dsYymbyZoGHmm-L6bznqVEPo5og&hl=en&sa=X&ved=2ahUKEwiW74PB3bj7AhU3qZUCHcdCBUIQ6AF6BQjoARAD
Additional information KnockOut serum replacement (KSR) is a brand name for an ... The exact formulation is proprietary, but its composition was published ...
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