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1 Immobilization of glucose isomerase - PubMed
https://pubmed.ncbi.nlm.nih.gov/6299015/
The immobilization of glucose isomerase (D-xylose ketol isomerase, EC 5.3.1.5) by covalently bonding to various carriers and by adsorption to ion exchange ...
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2 Immobilization of glucose isomerase onto radiation ...
https://www.sciencedirect.com/science/article/pii/S1687850714000077
It is well known that the stability of enzymes can be improved by immobilization because of diffusional resistance. P(AA-co-AMPS) and P(AA) supports should ...
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3 Immobilization of glucose isomerase and its application in ...
https://link.springer.com/article/10.1007/BF02788814
Bilayer glucose isomerase was immobilized in porousp-trimethylamine-polystyrene (TMPS) beads, through a molecular deposition technique.
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4 Immobilized glucose isomerase for the continuous conversion ...
https://pubs.acs.org/doi/10.1021/jf60201a018
Immobilized glucose isomerase for the continuous conversion of glucose to fructose · Cited By · Partners · About · Resources and Information.
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5 Immobilization of glucose isomerase - Wiley Online Library
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/jobm.19820220808
Various characteristics of free and immobilized enzymes such as the optimum pH, temperature and dependence of activity on storage and ...
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6 US4208482A - Immobilization of glucose isomerase
https://patents.google.com/patent/US4208482A/en
Immobilized glucose isomerase is prepared by mixing 0.5 to 1.5 parts by weight whole microbial cells containing glucose isomerase with one part by weight ...
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7 Immobilization of the glucose isomerase… - Digital CSIC
https://digital.csic.es/bitstream/10261/216977/1/Neifar_Immobilization_Food%20Chemistry_2020_Postprint.pdf
Biochemical properties of free and immobilized glucose isomerase. The support can significantly improve several enzyme features such as stability and ...
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8 Immobilization study of glucose isomerase - ProQuest
https://search.proquest.com/openview/a7a4a2e8de28f782ec221037e5dd84a6/1?pq-origsite=gscholar&cbl=18750&diss=y
Glucose isomerase (GI) catalyzes the isomerization of glucose to fructose and ... The GI is used in industry mainly in the form of immobilized enzyme.
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9 Immobilized Trienzymatic System with Enhanced Stabilization ...
https://www.mdpi.com/1420-3049/22/2/284/htm
The use of ketohexose isomerases is a powerful tool in lactose whey processing, but these enzymes can be very sensitive and expensive.
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10 latest.pdf - Research Square
https://www.researchsquare.com/article/rs-1496279/latest.pdf
covalently immobilising glucose isomerase (GI). ... The immobilisation approach ensures that the enzyme can be used continuously, making the.
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11 Development of an Immobilized Glucose Isomerase for ...
https://www.semanticscholar.org/paper/Development-of-an-Immobilized-Glucose-Isomerase-for-Hemmingsen/6f31c32cc27bac6f20b065a5ed50615712cfadb6
27 Citations · Activity of Glucose Isomerase from Bacillus thuringiensis under Different Treatments · Production, Purification, Immobilisation and ...
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12 Purification, Immobilization, and Some Properties of Glucose ...
https://journals.asm.org/doi/10.1128/aem.38.6.1111-1119.1979
Several differences in properties were found between purified soluble enzyme, immobilized enzyme (DEAE-cellulose-glucose isomerase), and heat-treated whole ...
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13 Ionic and covalent immobilization of glucose isomerase of ...
https://dergipark.org.tr/en/download/article-file/2101452
Afterward, kinetic and biochemical parameters of the immobilized enzymes were evaluated. ... glucose isomerase (GI) because of its ability to.
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14 Immobilized bilayer glucose isomerase in porous ...
https://pubs.rsc.org/en/content/articlelanding/1994/c3/c39940001297
Bilayer glucose isomerase was immobilized in porous trimethyamine polystyrene (TMPS) beads by molecular deposition based on electrostatic attraction, ...
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15 (PDF) Catalytic efficiency of immobilized glucose isomerase in ...
https://www.researchgate.net/publication/223056862_Catalytic_efficiency_of_immobilized_glucose_isomerase_in_isomerization_of_glucose_to_fructose
The catalytic efficiency of immobilized GI in isomerization of glucose to fructose was found as three fold higher than that of free GI. The ...
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16 Glucose Isomerase: Functions, Structures, and Applications
https://mdpi-res.com/d_attachment/applsci/applsci-12-00428/article_deploy/applsci-12-00428.pdf?version=1641202842
Glucose/xylose isomerase (GI/XI) is known to catalyze the reversible isomerization ... The commercially available immobilized.
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17 The use of immobilized enzymes in the food industry: A review
https://www.tandfonline.com/doi/abs/10.1080/10408397909527276
The production of high fructose corn syrups was greatly facilitated by the use of immobilized glucose isomerase. Similarly, in Japan, the ...
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18 Kinetics of glucose isomerization to fructose by ... - Europe PMC
https://europepmc.org/article/med/11090686
The substrate specificity of immobilized D-glucose isomerase (EC 5.3. 1.5) is investigated with an immobilized enzyme-packed reactor.
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19 Production of D-Allose From D-Allulose Using Commercial ...
https://www.frontiersin.org/articles/10.3389/fbioe.2021.681253/full
To address this limitation, we continuously produced D-allose from D-allulose using a packed bed reactor with commercial glucose isomerase ...
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20 Glucose isomerase - SlideShare
https://www.slideshare.net/reshvenkatesh/glucose-isomerase
Catalytic efficiency of immobilized glucose isomerase in isomerization of glucose to fructose. Food Chemistry, 111(3), 658–662. 22; 23.
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21 Kinetic study comparison of immobilized glucose ... - JMPAS
https://jmpas.com/admin/assets/article_issue/1629575994JMPAS_JULY-AUGUST_2021.pdf
conversion of glucose to fructose using Gensweet and Sweetzyme in Packed bed reactor (PBR) ... Enzymatic conversion using Immobilized-Glucose isomerase in.
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22 597. Glucose isomerase (non-immobilized & immob.) from S ...
https://inchem.org/documents/jecfa/jecmono/v20je07.htm
GLUCOSE ISOMERASE (non-immobilized and immobilized) from STREPTOMYCES RUBIGINOSIS EXPLANATION These enzymes preparations have not been previously reviewed ...
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23 Glucose Isomerase Patents and Patent Applications (Class ...
https://patents.justia.com/patents-by-us-classification/435/234?page=2
Method for maintaining immobilized glucose isomerase activity during continuous isomerization of glucose to fructose ... The method is preferably carried out when ...
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24 Enzymes for isomerization - Novozymes
https://biosolutions.novozymes.com/en/grain-starch/isomerization
Production of fructose syrup requires converting glucose into a mixture of glucose and fructose by running the glucose syrup through a bed of immobilized ...
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25 Bioconversion of d-glucose to d-psicose with immobilized d ...
https://academic.oup.com/jimb/article/42/8/1117/5961487
cerevisiae spore wall. In this study, we employed biological and chemical approaches for the immobilization of d-xylose isomerase (XI) from Thermus thermophilus ...
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26 [33] Industrial-scale production and application of immobilized ...
https://www.scinapse.io/papers/113222186
Better immobilized glucose isomerase (IGI) products allowed improvements in the isomerization process, leading to further development of more sophisticated ...
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27 Isolation of Glucose Isomerase Hyperproducing Strain ...
https://www.koreascience.or.kr/article/JAKO199211922230101.pdf
SM 805, showed the greatest ability to produce glucose isomerase. ... resultant immobilized enzyme pellets showed a relatively long stability during the ...
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28 Production of High Fructose Syrup by Glucose Isomerase ...
https://pubag.nal.usda.gov/catalog/5851926
An immobilized glucose isomerase which is active and stable in concentrated glucose solution has been developed. The enzyme has been immobilized to Duolite A7 ...
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29 B8A01799 - Immobilised Glucose Isomerase - 50g - Philip Harris
https://www.philipharris.co.uk/product/biology/immobilised-glucose-isomerase-50g/b8a01799
Working Concentration - dilution of the Enzyme (as supplied) to this level should give an acceptable reaction time. Substrate - substance to be reacted with the ...
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30 Safety evaluation of the food enzyme glucose isomerase from ...
https://www.efsa.europa.eu/en/efsajournal/pub/5547
... Novozymes A/S. The glucose isomerase is intended only to be used in an immobilised form in glucose isomerisation for the production of ...
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31 FDA response on HFCS - Enzyme Technical Association
https://www.enzymetechnicalassociation.org/wp-content/uploads/2018/10/FDA-response-on-HFCS.pdf
glucose (dextrose) to fructose using an insoluble glucose isomerase enzyme ... use of the enzyme, and that after the immobilized enzymes are fixed in place, ...
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32 Enhancement of glucose isomerase activity by pretreatment ...
https://www.cheric.org/PDF/KJChE/KC28/KC28-4-1096.pdf
with D-glucose or D-xylose to prevent loss of activity, followed by immobilization on a silica gel surface. Pretreated immobilized glucose isomerase (PIGI) ...
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33 Properties and application of Sweetzyme T - EurekaMag
https://eurekamag.com/research/001/921/001921605.php
Properties and application of Sweetzyme T - a new immobilized glucose isomerase produced by a strain of Streptomyces murinus ... Pedersen, S.; Rugh, S.
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34 Method for maintaining immobilized glucose isomerase activity...
https://www.mysciencework.com/patent/show/method-maintaining-immobilized-glucose-isomerase-activity-during-continuous-isomerization-glucose-fructose-US5177005A
The method is preferably carried out when continuously isomerizing glucose to fructose with glucose isomerase adsorbed to a weakly basic anion exchange resin.
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35 Glucose Isomerase - Creative Enzymes
https://www.creative-enzymes.com/similar/glucose-isomerase_319.html
These include (i) chemical modification, (ii) X-ray crystallography, and (iii) isotope exchange. The main features of the mechanism proposed for GI are ring ...
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36 Kinetics of a Three-Step Isomerization of Glucose to Fructose ...
http://www.ijcea.org/papers/255-CA024.pdf
Immobilized glucose isomerase from Strepomyces murinus was used in a packed bed reactor to obtain fructose from glucose and the kinetics of ...
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37 Immobilization of glucose isomerase and its application in ...
https://agris.fao.org/agris-search/search.do?recordID=US1997071475
Bilayer glucose isomerase was immobilized in porous ... with the enzyme concentration of 308 IU/mL, enzyme-to-matrix ratio of 924 IU/g wet carrier, ...
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38 Conversion of Glucose in Lactase-Hydrolyzed Whey ...
https://www.journalofdairyscience.org/article/S0022-0302(86)80488-X/pdf
with Immobilized Glucose Isomerase. ABSTRACT. The maximum conversion of gIucose to fructose in lactase-hydrolyzed whey permeate by glucose isomerase was.
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39 Mathematical modeling of fructose production by immobilised ...
https://www.ajol.info/index.php/ajb/article/view/137983/127553
The solid phase is referred to as the immobilized glucose isomerase and the glucose solution as the liquid phase. Most of the researchers used ...
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40 The use of immobilized enzymes in the food industry: A review
https://college.agrilife.org/talcottlab/wp-content/uploads/sites/108/2019/01/Enzyme-Immobilization.pdf
Although a wide variety of enzymes can be immobilized by entrapment, ... coupling the enzyme to porous glass beads.124 Glucose isomerase ...
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41 Enhancing the Thermostability of Glucose Isomerase ... - Nature
https://www.nature.com/articles/nbt0891-738
We have engineered recombinant glucose isomerase (GI) from Actinoplanes missouriensis by site-directed mutagenesis to enhance its thermal ...
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42 F6301035039.pdf - IJERA
http://www.ijera.com/papers/Vol6_issue3/Part%20-%201/F6301035039.pdf
molasses was selected. The isomerisation of glucose to fructose experiments was conducted using soluble and immobilized glucose Isomerase enzyme.
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43 Kinetics of Glucose Isomerization to Fructose by Immobilized ...
https://www.academia.edu/9552154/Kinetics_of_Glucose_Isomerization_to_Fructose_by_Immobilized_Glucose_Isomerase_Sweetzyme_IT
The investigated catalyst system consists of immobilized Streptomyces bambergiensis cells containing the enzyme glucose isomerase, which catalyzes the ...
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44 Current applications of immobilized enzymes for ...
https://www.nottingham.ac.uk/ncmh/documents/bger/volume-1/bger1-5.pdf
In 1976, 750 tonnes (t) of immobilized glucose isomerase were used to produce around 800000t of 42% fructose in the US alone. Thus, by the time that the.
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45 High-fructose corn syrups (HFCS)
https://water.lsbu.ac.uk/water/enztech/hfcs.html
All glucose isomerases are used in immobilised forms. Although differerent immobilisation methods have been used for enzymes from differerent ...
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46 One-step conversion of cellulose to fructose using ...
http://kenstoreylab.com/wp-content/uploads/2016/03/One-step-conversion-of-cellulose-to-fructose-using-coimmobilized-cellulase-%CE%B2-glucosidase-and-glucose-isomerase.pdf
Glucose isomerase was immobilized by itself and coimmobilized with cellulase and fl-glucosidase using a polyurethane foam (Hypol |. FHP 2002).
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47 Design of operational temperature for immobilized glucose ...
https://sciendo.com/pdf/10.2478/acs-2018-0022
Keywords: high-fructose corn syrup, glucose isomerase, process stability, inactivation kinetics. Introduction. Reversible isomerization of D-glucose to D- ...
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48 Novel Recyclable UCST-Type Immobilized Glucose ... - Figshare
https://figshare.com/collections/Novel_Recyclable_UCST-Type_Immobilized_Glucose_Isomerase_Biocatalyst_with_Excellent_Performance_for_Isomerization_of_Glucose_to_Fructose/6258509
The development of a suitable immobilization strategy to improve the performance of immobilized glucose isomerase for the isomerization of ...
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49 final scientific/technical report - OSTI.GOV
https://www.osti.gov/servlets/purl/813693
process, an enzyme catalyst is used to convert glucose to the much ... immobilized glucose isomerase preparations were developed along with.
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50 Xylose isomerase, a method for production of such xylose ...
https://patentimages.storage.googleapis.com/be/b7/18/c7ae4df3fd3d5b/EP0194760B1.pdf
immobilized xylose isomerase and a method for isomerization of glucose to fructose. 5. Enzymes capable of isomerizing glucose to fructose i.e. glucose ...
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51 Immobilization of glucose isomerase onto radiation ...
https://cyberleninka.org/article/n/463895
Abstract Isomerization of glucose to fructose was carried out using Glucose isomerase (GI) that immobilized by entrapment into Poly(acrylic acid) P(AA) and ...
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52 Process of preparing glucose isomerase - Google Patents
https://www.google.com/patents/USRE29692
with the preferred range being about 60° to 75° C. The enzyme activity is enhanced by the addition of small quantities of divalent cations such as magnesium ...
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53 Production of High Fructose Syrup (HFS) with immobilized ...
http://www.glicoenz.org/2019/11/production-of-high-fructose-syrup-hfs.html
Production of High Fructose Syrup (HFS) with immobilized glucose isomerase published in Food Chemistry ... In collaboration with University of ...
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54 Covalent immobilization of glucose isomerase on poly(2 ...
https://open.metu.edu.tr/handle/11511/14447
For maximum substrate conversion, optimum temperature was determined as 70 oC for free GI and this value shifted to 60 oC for immobilized enzyme. Optimum pH for ...
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55 Manufacture of High Fructose Corn Syrup Using Immobilized ...
https://www.taylorfrancis.com/chapters/edit/10.1201/9781351073400-4/manufacture-high-fructose-corn-syrup-using-immobilized-glucose-isomerase-macallister
In this chapter the events which led up to the development of processes for ... Manufacture of High Fructose Corn Syrup Using Immobilized Glucose Isomerase.
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56 Immobilized Enzymes for Waste Stream Valorization
https://ecommons.cornell.edu/handle/1813/59598
These waste streams, rich in sugars, proteins, and lipids, can be ... and glucose isomerase (E.C. 5.3.1.5) were co-immobilized within a ...
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57 Use of Immobilized Glucose Isomerase Enzyme in ... - Jetir.Org
https://www.jetir.org/view?paper=JETIR1905L45
Isomerization of glucose to fructose, a reversible reaction catalyzed by isomerase enzyme, is a very important industrial process to produce high fructose ...
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58 Applications of Immobilized Enzymes - Biotech Articles
https://www.biotecharticles.com/Biotech-Research-Article/Applications-of-Immobilized-Enzymes-880.html
High fructose syrup can be produced from glucose by employing an immobilized enzyme glucose isomerase. The starch containing raw materials ...
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59 Chapter 5: Immobilised enzymes and their uses
https://www.dbt.univr.it/documenti/OccorrenzaIns/matdid/matdid174369.doc
Only an isomerase that would use underivatised glucose as its substrate would be commercially useful but, until the late 1950s, the existence of such an enzyme ...
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60 Scaling up an Immobilized Enzyme System - JSTOR
https://www.jstor.org/stable/1697722
sorbed onto water-insoluble matrices such that they can be separated ... By the late 1970's, immobilized glucose isomerase had become.
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61 Immobilized Glucose Isomerase enzyme - Antozyme Biotech
https://antozymebiotech.com/immobilized-glucose-isomerase-enzyme/
improvement of the yields of Glucose isomerase, · optimization of the fermentation medium with particular reference to replacement of xylose by a cheaper ...
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62 Glucose Isomerase from Streptomyces murinus - Sigma-Aldrich
https://www.sigmaaldrich.com/US/en/product/sigma/g4166
Glucose Isomerase from Streptomyces murinus has been used to isomerize xylose to xylulose during the production of dihydrogen from Xylose.
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63 Glucose Isomerization in Simulated Moving Bed Reactor by ...
https://www.scielo.br/j/babt/a/XMSyMGYzjJbPnrdq9kBdkBG/?lang=en&format=pdf
conversion using the immobilized enzyme glucose isomerase were investigated. ... isomerization reaction of the glucose to fructose.
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64 Development and verification of a model to describe an ...
https://www.infona.pl/resource/bwmeta1.element.elsevier-5f0dbbde-23d1-324e-b8c8-e81fdd721c3e
In this paper, the performance of immobilized packed bed glucose isomerase enzyme was mathematically modeled. A modified Michaelis–Menten type relation was ...
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65 Immobilized glucose isomerase
http://mbio.bas-net.by/en/prod/preparat-immobilizovannoj-glyukozoizomerazy/
Immobilized glucose isomerase · Purpose: Production of natural sugar substitutes for dietetic and prophylactic- therapeutic nutrition – glucose-fructose syrup ...
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66 Immobilised Glucose Isomerase - 50g - Hope Education
https://www.hope-education.co.uk/product/science/biology/immobilised-glucose-isomerase-50g/he1006279
Working Concentration - dilution of the Enzyme (as supplied) to this level should give an acceptable reaction time. Substrate - substance to be reacted with the ...
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67 Development and verification of a model ... - Monash University
https://research.monash.edu/en/publications/development-and-verification-of-a-model-to-describe-an-immobilize
In this paper, the performance of immobilized packed bed glucose isomerase enzyme was mathematically modeled. A modified Michaelis-Menten ...
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68 Microwave irradiation-assisted isomerization of glucose to ...
https://kd.nsfc.gov.cn/paperDownload/1000004339795.pdf
The isomerization of glucose to fructose by immobilized glucose isomerase (IGI, produced from Strep- tomyces murinus and immobilized on silica) was ...
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69 glucose isomerase from immobilized arthrobacter globiformis ...
http://www.thegoodscentscompany.com/data/rw1608641.html
Category: enzyme preparations and microorganisms. Recommendation for glucose isomerase from immobilized arthrobacter globiformis usage levels up to:.
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70 Enzyme Immobilisation-Leaving Cert Biology - YouTube
https://www.youtube.com/watch?v=oiTVJ91-YxQ
Biology Bugbears
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71 Biotechnology for Beginners - Page 48 - Google Books Result
https://books.google.com/books?id=lDYL6793vMkC&pg=PA48&lpg=PA48&dq=how+can+glucose+isomerase+be+immobilised&source=bl&ots=TStfXgpEp7&sig=ACfU3U3OhmEe_Rtote9-h2qAOK6IFvPHTg&hl=en&sa=X&ved=2ahUKEwjxuY6XlsD7AhUplYkEHRF7D_8Q6AF6BQiNAhAD
Fortunately , glucose isomerase is an ideal enzyme to be immobilized , as it ... so when the immobilized enzyme is packed into columns , diffusion problems ...
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72 Advanced Biotechnology - Page 692 - Google Books Result
https://books.google.com/books?id=SKgrDAAAQBAJ&pg=PA692&lpg=PA692&dq=how+can+glucose+isomerase+be+immobilised&source=bl&ots=FQNIwvEgHn&sig=ACfU3U3aREz0obJIhsY9kUjh3BqV1mGPqg&hl=en&sa=X&ved=2ahUKEwjxuY6XlsD7AhUplYkEHRF7D_8Q6AF6BQiUAhAD
Some of the immobilized preparations are: enzyme immobilised by ionic binding to ... Conversion of glucose to fructose 50% fructose. by glucose isomerase.
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