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1 How do SPRI beads work? - Enseqlopedia
http://enseqlopedia.com/2012/04/how-do-spri-beads-work/
Each bead is made of polystyrene surrounded by a layer of magnetite, which is coated with carboxyl molecules. It is these that reversibly bind DNA in the ...
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2 PEG Precipitation of DNA Libraries – How Ampure or ...
https://blog.genohub.com/2014/05/07/peg-size-selection-and-precipitation-of-dna-libraries-how-ampure-or-spriselect-works/
Polystyrene – magnetite beads (Ampure) are coated with a layer of negatively charged carboxyl groups. DNA's highly charged phosphate backbone ...
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3 Agencourt AMPure XP
https://research.fredhutch.org/content/dam/stripe/hahn/methods/mol_biol/Agencourt%20AMPure%20XP.pdf
This step binds DNA fragments 100 bp and larger to the magnetic beads. Pipette mixing is preferable to vortexing as it tends to be more reproducible. The color ...
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4 SPRI Beads-based Size Selection in the Range of 2-10kb - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944320/
Both, the salts and the PEG in SPRI buffer play critical role in forming DNA precipitate on magnetic beads. Salts provide ion “bridging” of negatively charged ...
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5 AMPure Purification Protocol - Protocols.io
https://www.protocols.io/view/ampure-purification-protocol-bn39mgr6
SPRI beads are paramagnetic and coated with carboxyl molecules, which reversibly bind DNA in the presence of polyethylene glycol (PEG) and salt (20% PEG, ...
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6 SPRI Bead Technology - Beckman Coulter
https://www.beckman.com/resources/technologies/spri-beads
SPRI technology uses paramagnetic beads to selectively bind nucleic acids by type and size, and are used for high-performance isolation, purification, ...
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7 Next-Generation Sequencing Tips n' Tricks - Part 2
https://diagnostech.co.za/next-generation-sequencing-tips-n-tricks-part-2/
Basic principle of SPRI beads · Add and mix 1.8 μL AMPure XP per 1.0 μL of sample (e.g. 90 μL beads to 50 μl sample). · Bind DNA fragments to ...
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8 Questions about how dose Ampure xp beads work
https://www.researchgate.net/post/Questions-about-how-dose-Ampure-xp-beads-work
So they serve as a precipitation point but the DNA needs to be precipitated by sth. Usually one uses high salt and PEG precipitation (similar to ...
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9 SPRI Magnetic Beads for DNA Purification - AG Scientific
https://agscientific.com/blog/2021/03/dna-purification-spri-magnetic-beads/
How does magnetic bead DNA extraction work? SPRI beads usually comprise a polystyrene core surrounded by magnetite (Fe3O4), which causes the beads to clump when ...
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10 DNA purification on AMPure XP beads
https://www.babraham.ac.uk/sites/default/files/2020-07/AMPure%20clean-up.doc
This method is standard for purification of DNA libraries and intermediates during preparation of Illumina libraries. This protocol is basically as recommended ...
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11 Magbeads 101: A guide to choosing and using magnetic beads
https://www.cytivalifesciences.com/en/us/news-center/magnetic-beads-a-simple-guide-10001
How does magnetic bead DNA extraction work? · Reversibly binds nucleic acids based on salt concentration. · Monodisperse particles with narrow ...
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12 AMPure XP beads DNA purification - GitHub
https://github.com/repseqio/protocols/blob/master/AMPure%20beads%20purification%20protocol.md
Agencourt AMPure XP utilizes an optimized buffer to selectively bind DNA fragments 200 bp and larger to paramagnetic beads. Excess primers, nucleotides, salts, ...
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13 SPRI bead mix - - OpenWetWare
https://openwetware.org/wiki/SPRI_bead_mix
Add 1 mL of previously prepared "DNA buffer" or "RNA buffer", depending on the kind of bead binding mix you are preparing, to the bead pellet and close the tube ...
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14 Iyer Laboratory: Gel-free Library Preparation Protocol
https://repositories.lib.utexas.edu/bitstream/handle/2152/47310/2017_06_12_gel_free_library_prep_protocol.pdf?sequence=3&isAllowed=y
Ampure beads are magnetic beads that bind DNA (using PEG) in specific size ranges. When the PEG solution (supernatant) is removed, DNA can be eluted off of ...
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15 Ampure versus Ampure XP - SEQanswers
https://www.seqanswers.com/forum/applications-forums/sample-prep-library-generation/3018-ampure-versus-ampure-xp
In AMPure beads, the magnetite is sealed by a "non-styrene polymer"; it is unclear from the figure whether XP beads are sealed with something ...
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16 Maximize the Efficiency of DNA Purification with Magnetic Beads
https://www.magbiogenomics.com/latest/post/maximize-the-efficiency-of-dna-purification-with-magnetic-beads
The most striking ability the beads possess is to achieve Solid-Phase Reversible Immobilization (SPRI). Simply put, they can reversibly bind ...
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17 Sample Preparation - Broad Institute
https://www.broadinstitute.org/files/shared/illuminavids/SamplePrepSlides.pdf
Attach adapters to both ends of fragmented nucleic acid. Library Preparation ... SPRI technology is a magnetic bead based DNA clean up method.
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18 DNA Cleanup for NGS Library: Basics, Methods and Examples
https://www.nvigen.com/dna-clean-up/
DNA clean-up, also known as magnetic beads-enabled cleanup, is the targeted removal of small DNA fragments such as primers, adapters and dimers from a ...
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19 Magnetic Beads (DNA & RNA Purification) - BioDynami
https://biodynami.com/product/magnetic-beads-dna-rna-purification/
Solid Phase Reversible Immobilization magnetic beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. They are used for ...
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20 Library size selection using Sample Purification Beads
https://support.illumina.com/bulletins/2020/07/library-size-selection-using-sample-purification-beads.html
The large fragments are bound to the beads and left behind, while the library of interest remains in the supernatant and is transferred to a new well. New beads ...
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21 Shared Protocol - 10 kb to 20 kb Template Preparation and ...
https://www.pacb.com/wp-content/uploads/2015/09/Shared-Protocol-10-kb-to-20-Kb-Template-Preparation-with-Low-Input-DNA.pdf
shearing methods and subsequent AMPure PB bead purification steps ... mix the DNA with the bead reagent will result in inefficient DNA binding and.
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22 AMPure bead cleanup
https://www.umassmed.edu/globalassets/deep-sequencing-core/protocols/ampure-bead-cleanup.pdf
DeepSeq Core AMPure Purification Protocol. Page 1 of 1 ... Ampure XP beads. Lo-Bind tubes ... To recover lost DNA from supernatant, do a 1X bead wash.
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23 Unlocking Short Read Sequencing for Metagenomics - PLOS
https://journals.plos.org/plosone/article/figures?id=10.1371/journal.pone.0011840
AMPure XP SPRI beads bind DNA fragments in a size dependent manner according to the concentration of salts and polyethylene glycol (PEG) in the reaction ...
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24 QIAGEN Supplementary Protocol
https://www.qiagen.com/se/resources/download.aspx?id=de911f9f-db0c-462a-a73f-5261eb1366e5&lang=en
Purification of REPLI-g® amplified DNA using. Agencourt® AMPure® XP magnetic beads. This protocol is designed for the purification of 5–40 μg DNA amplified ...
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25 How do SPRI beads bind DNA : r/Biochemistry - Reddit
https://www.reddit.com/r/Biochemistry/comments/qi88j5/how_do_spri_beads_bind_dna/
Digging around some more it appears that the PEG can induce a coil-to-globule state change in the DNA with the NaCl helping to reduce the ...
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26 SPRI Cutting Edge Magnetic Bead Technology - Labplan
https://labplan.ie/content/uploads/2018/03/Agencourt_SPRI_Datasheet.pdf
SPRI beads are constructed using a core shell process that ... binding capacity for nucleic acids. ... Genomic DNA – PCR and. Genotyping.
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27 SPRIselect:DNA Ratios Affect the Size Range of Library ...
https://assets.ctfassets.net/an68im79xiti/4kamLcb2l2ieaoa0cKcosi/f69fc2cfe0326377294aa616b9681b2d/CG000061_10x_Technical_Note_SPRIadjustment_-_v2.pdf
Solid Phase Reversible Immobilization (SPRI) beads selectively bind DNA fragments ... beads to enrich for DNA fragments with a specific size range that is ...
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28 IrwinLabGBSprotocolv2.2Sep-2017.docx - UBC Zoology
https://www.zoology.ubc.ca/~irwin/irwinlab/wp-content/uploads/2018/06/IrwinLabGBSprotocolv2.2Sep-2017.docx
The binding capacity of SPRI beads is huge. 1ul of AmpureXP will bind over 3ug DNA. This is the typical SPRI protocol from Beckmans website.
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29 How are the ratios of SPRIselect beads calculated in 10x ...
https://kb.10xgenomics.com/hc/en-us/articles/360050060992-How-are-the-ratios-of-SPRIselect-beads-calculated-in-10x-assays-
1), during 1st SPRI cleanup at 0.6X, SPRI beads selectively bind DNA fragments larger than 600 bp, and the beads are discarded. During the 2nd SPRI cleanup, ...
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30 Evaluation of Omega Mag-Bind® TotalPure NGS Beads for ...
https://gc3fstorage.uoregon.edu/IMAGES/Evaluation_of_Omega_Mag-Bind_TotalPure_NGS_Beads_MWeitzman_April2018.pdf
Carboxyl-coated paramagnetic beads such as AMPure, SPRISelect, and Omega Mag-Bind are frequently used in molecular biology to purify DNA from within ...
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31 Molecular Biology - Bangs Laboratories
https://www.bangslabs.com/sites/default/files/imce/docs/TechNote%20302%20Web.pdf
Note: The SPRI PCR method binds DNA based on size, from 200bp to 200 ... group and the beads are surface-functionalized with either amine groups or.
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32 Xtra Performance Post-PCR1 Cleanup - Agencourt AMPure ...
https://www.n-genetics.com/products/1012/1024/12716.pdf
The Agencourt AMPure XP kit is a highly efficient PCR purification system that delivers superior quality DNA with no salt carryover. The Agencourt AMPure ...
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33 Solid-phase reversible immobilization - Wikipedia
https://en.wikipedia.org/wiki/Solid-phase_reversible_immobilization
Solid-phase reversible immobilization, or SPRI, is a method of purifying nucleic acids from solution. It uses silica- or carboxyl-coated paramagnetic beads, ...
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34 How should I purify my samples? How should I remove DNA ...
https://dnatech.genomecenter.ucdavis.edu/faqs/how-should-i-purify-my-samples-how-should-i-remove-dna-or-rna-contamination/
How should I remove DNA or RNA contamination? Bead based sample cleanups (e.g., Ampure XP, RNAClean XP) and spin column-based protocols (e.g., Qiagen, Zymo, ...
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35 Tips to work with SPRI (AMPure) beads in NGS library preps
https://sites.psu.edu/yuka/2017/12/21/tips-to-work-with-spri-ampure-beads-in-ngs-library-preps/
0.6x binds things >400bp so this is often used to get rid of bigger fragment in 'dual size selection' setup. The supernatant after 0.6x contains ...
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36 NGS Magnetic Beads - Sample and Library Preparation Kits
https://www.abmgood.com/DNA-Purification-SPRI-Magnetic-Beads.html
abm's DNA Purification Magnetic Beads are formulated to bind specifically to DNA and allow the removal of unwanted excess primers, adapter dimers, ...
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37 Harnessing the MinION: An example of how to establish long ...
https://onlinelibrary.wiley.com/doi/full/10.1111/1755-0998.12938
For the last postadapter ligation step, SPRI beads were washed with ... These beads bind to the DNA, but most contaminants do not and can be ...
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38 AGENCOURT
https://genome.med.harvard.edu/documents/sequencing/Agencourt_AMPure_Protocol.pdf
AGENCOURT AMPURE is a registered trademark of Agencourt Bioscience and is for ... to selectively bind PCR amplicons 100bp and larger to paramagnetic beads.
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39 Covaris - Magnetic Bead-based Clean-up Using AFA-energetics
https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020088.pdf
Introducing Covaris AFA into the binding and elution steps of SPRI ... applied during bead binding and bead elution, the DNA yields are.
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40 CleanNGS DNA & RNA Clean-Up Magnetic Beads - Bulldog-Bio
https://www.bulldog-bio.com/product/cleanngs-spri-beads/
CleanNA bead technology works by allowing binding buffers to control the size of nucleic acids that will bind to the beads. Unlike inferior anion exchange beads ...
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41 Axygen® AxyPrep Magnetic Bead Purification Kits Brochure
https://www.corning.com/catalog/cls/documents/brochures/CLS-A-BS-012_REV2_DL.pdf
The purification of high quality DNA/RNA is the first essential step for any genomics- ... AxyPrep MAG PCR Normalizer beads have limited binding surface,.
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42 Nanopore Sequencing Book: DNA extraction and purification ...
https://lab.loman.net/2018/05/25/dna-extraction-book-chapter/
A commonly used technique is the use of solid-phase reversible immobilization beads (SPRI). DNA binds to the beads in the presence of the bead ...
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43 Beads-free ONT ligation kit library preparation for ultra-long ...
https://gih.uq.edu.au/research/long-read-sequencing/beads-free-ont-ligation-kit-library-preparation-ultra-long-read-sequencing
In this method, AMPure beads are replaced by the PEG/NaCl solution and short read eliminator (SRE) XL kit to keep DNA intact during the sequencing library ...
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44 Beckman Coulter AMPure XP PCR Cleanup and Size Selection
https://protocols.opentrons.com/protocol/53fec2
Protocol Steps · Binding buffer is mixed and pre-added to the magnetic module plate. · Corresponding columns from each sample plate are consolidated into the ...
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45 Table 1 Binding efficiencies of single-stranded DNA (ssDNA ...
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1495-0/tables/1
› tables
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46 VAHTS® DNA Clean Beads - Vazyme
https://www.vazymebiotech.com/uploads/N411-V21.1.pdf
The usage of VAHTS DNA Clean Beads is the same as the AMPure ... Incubate at room temperature for 10 min to bind DNA to magnetic beads.
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47 sparQ PureMag Beads | Quantabio
https://www.quantabio.com/wp-content/uploads/2021/12/MK-SF-0030_REV02_sparQ_PureMag_Beads_0121_lr.pdf
beads. Add beads. Pellet. Wash 2x. Transfer to a new tube. Beads preferentially bind to large DNA fragments which will be removed later;.
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48 US20090275486A1 - Nucleic acid separation and purification ...
https://patents.google.com/patent/US20090275486A1/en
Polybrene will bind negatively charges molecules (DNA and carboxylated beads) which can then be placed on magnetic stand and washed with H2O.
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49 A Microfluidics Workflow for Sample Preparation for Next ...
https://journals.sagepub.com/doi/pdf/10.1177/2472630318796133
A total of 11 µL of reagent A (magnetic beads and binding buffer) is added and incubated for. Δt2 = 5 min. The beads are moved to W2. DNA elutes from the ...
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50 Dynabeads Nucleic Acid Purification Support—Troubleshooting
https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/nucleic-acid-purification-analysis-support-center/dynabeads-nucleic-acid-purification-support/dynabeads-nucleic-acid-purification-support-troubleshooting.html
The binding capacity of streptavidin-coupled Dynabeads magnetic beads is fragment length-dependent. Reduced binding capacity for large DNA or RNA fragments may ...
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51 RK20202 StepWise DNA Lib Prep Kit Protocol - INTERCHIM
https://www.interchim.fr/ft/B/B2YI90.pdf
The StepWise DNA Library Preparation Kit is designed to generate high-quality ... AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. A63880).
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52 Ampure Xp Beads Purification Protocol
https://prevost-architectural.com/wp-content/uploads/formidable/6/ampure-xp-beads-purification-protocol.pdf
beads that bind DNA using PEG in specific size ranges. This protocol is indistinguishable from ampure xp magnetic particles that you for protocols on the.
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53 NucleoMag NGS Clean-up and Size Select - Takara Bio
https://www.takarabio.com/products/nucleic-acid-purification/dna-cleanup-kits/nucleomag-ngs-clean-up-and-size-select
Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This ...
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54 Illumina Dye Sequencing - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/illumina-dye-sequencing
Once prepared, the DNA fragments are washed across the flowcell. The complementary DNA binds to primers on the surface of the flowcell and DNA that does not ...
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55 Simple amplicon sequencing library preparation for plant root ...
https://www.biorxiv.org/content/10.1101/2021.04.14.439905v1.full.pdf
PCR purification (AMP_AMP), and AMPure XP beads method for DNA extraction and. (which was not certified by peer review) is the author/funder ...
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56 Affymetrix User Bulletin, AMPure XP Beads for SNP 6.0 Assay
https://www.affymetrix.com/support/technical/product_updates/ampure_xp_beads_snp6_bulletin.pdf
This document is intended for customers who are using the original SNP Assay 6.0 Kit. ... minute incubation, the DNA binds to the magnetic beads.
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57 Application notes using GenScript's magnetic beads
https://www.genscript.com/application-notes-magnetic-beads.html
Due to accumulation of impurities and leaching of ligands with each cycle of purification, a reduced binding capacity is observed with each round of reuse. The ...
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58 Magnetic Beads Size Selection | Mag-Bind® TotalPure NGS
https://www.omegabiotek.com/product/ngs-workflow-pcr-clean-up-mag-bind-total-pure-ngs/
Can Mag-Bind TotalPure NGS be used for “High Molecular Weight (HMW)” DNA?
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59 MagSi Magnetic Beads for Genomic Applications - AMSBIO
https://www.amsbio.com/magsi-magnetic-beads-genomics/
Magnetic beads can be used as solid support phase in DNA extraction and purification protocols by a simple bind/wash/elute principle.
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60 Product Info GK19 V1.0 – 01/2018 1 GRiSP Research ...
https://bocascientific.com/images/pdf/GK19.0005-xpert-purification-spri-magnetic-beads-datasheet.pdf
been optimized in order to selectively bind DNA fragments of 100bp and larger. ... Moreover, SPRI beads can be seamlessly integrated.
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61 Sample Requirements: Long and Linked Read Sequencing ...
https://www.ramaciotti.unsw.edu.au/sites/default/files/2019-04/RAMAC_Long_Linked_Read_guidelines_2019.pdf
inactivation where possible as overheating can introduce DNA damage. An alternative method is. AMPure® XP bead purification. It is essential that XP beads ...
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62 Genome-wide Nucleotide-Resolution Mapping of DNA ...
https://www.cell.com/cms/10.1016/j.molcel.2020.03.027/attachment/ee62a6e5-f484-42d7-aef7-36c7c7c3879d/mmc1
using AMPure beads) are applied at this stage to generate the ... Clean up the DNA by two rounds of binding to an equivalent of 100 μL of AMPure beads, ...
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63 Using the C.WASH to conduct automated, non-contact bead ...
https://www.cellink.com/wp-content/uploads/2021/03/CWASH-Bead-Clean-Up-TechNote-D-20210314.pdf
DNA purification of PCR products is a critical step in the NGS library ... to sample solution is used to preferentially bind only one thing to the beads.
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64 Beer DNA extraction - Street Science Community
https://streetscience.community/protocols/beer-dna-extraction/
To extract the DNA, we will make the DNA binding to some beads and then use a magnet to capture the beads with the DNA. Then, we wash and repeat pretty much the ...
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65 ATAC Sequencing Protocol Kaestner Lab Date revised
https://hpap.pmacs.upenn.edu/assets/workflow/Islet%20Molecular%20Phenotyping%20Studies/ATAC%20Sequencing.pdf
Agencourt AMPure XP magnetic beads (Beckman Coulter, catalog # A63880). 16. 80% Ethanol (made fresh). 17. Agilent High Sensitivity DNA ...
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66 DNA Purification SPRI Magnetic Beads - Coderegenesis
https://www.coderegenesis.com/product/dna-purification-spri-magnetic-beads-25-ml/
The beads work by binding DNA reversibly to carboxyl-coated paramagnetic particles, allowing for high recovery of DNA using a quick and simple procedure. They ...
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67 DNA Clean-Up and Size Selection - BioCat GmbH
https://www.biocat.com/genomics/next-generation-sequencing-related-dna-purification-and-size-selection/dna-clean-up-and-size-selection
The EpiNext™ DNA Purification HT System contains an optimized MQ binding bead solution which allows DNA or PCR amplicons to bind tightly to the beads. Excess ...
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68 A Magnetic Bead-Based DNA Extraction Protocol Suitable for ...
https://www.genaqua.org/pdf.php?&id=30
While convenient, the expense of using such kits can be ... compromised DNA binding to the magnetic beads or ... or Agencourt AMPure-XP beads.
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69 Instructions For Use FormaPure DNA: - Berner Brand Bank
https://www.bernerbrandbank.fi/l/z9bPDDjFVfQp
RNA is removed from the sample and a binding solution is added to immobilize the nucleic acids to the surface of the SPRI beads. Contaminants are rinsed away ...
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70 ADP1Tn-seqLibraryPrep - Barrick Lab
https://barricklab.org/twiki/bin/view/Lab/ADP1Tn-seqLibraryPrep
In a low retention eppindorf tube, combine DNA and beads in the appropriate ratio. For this step, a 1.2 bead:DNA ratio is required. To do this, ...
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71 NEBNext DNA Sample Prep Master Mix Set for 454 Fill-in and ...
https://international.neb.com/protocols/0001/01/01/nebnext-fill-in-and-ssdna-isolation-module-protocol-e6070-e6071
Removal of small fragments using Agencourt AMPure Beads or gel size selection. ... Wash beads twice with 100 μl of 2X Bead Binding Buffer, pelleting the ...
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72 Automation of Agencourt AMPure Purification Kit for ... - Agilent
https://www.agilent.com/cs/library/applications/5990-4942en_lo%20CMS.pdf
Described below is a protocol for the Agilent Bravo. Automated Liquid Handling Platform capable of automatically handling the binding, washing, ...
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73 SeqPure PCR Purification System | BioChain
https://www.biochain.com/wp/wp-content/uploads/resources/seqpure-pcr-purification-system-(fk180306).pdf
Lane 5: Ampure beads with pre-warmed TE. 1. SeqPure beads are added to the. PCR product for. DNA binding. 2. During the process, contaminants and salts are.
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74 High molecular weight gDNA extraction.
https://db.cngb.org/dc_assets/build/dc_10kp/data/High%20molecular%20weight%20gDNA%20extraction%20method.pdf
Extraction of high-molecular-weight genomic DNA for the long-read ... Note: AMPURE XP (Beckman Coulter, Brea, CA, USA) can be used but they must be washed 4 ...
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75 Data sheet - Canvax
https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2018/02/HigherPurity%E2%84%A2-SPRI-Magnetic-Beads.pdf
SPRI beads work by binding DNA ... formulated with an optimized buffer to selectively bind. DNA ... DNA Purification SPRI Magnetic Beads can be easily.
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76 Canvax HigherPurity™ DNA Purification SPRI Magnetic ...
https://www.labunlimited.com/s/ALL/CX-AN360/Canvax-HigherPurity%E2%84%A2-DNA-Purification-SPRI-Magnetic-Beads-AN360
The Magnetic beads are pre-formulated with an optimized buffer to selectively bind DNA fragments of 100 bp and larger. Excess salts, enzymes, primers and ...
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77 Multiplexed Strand-specific RNA-Seq Library Preparation for ...
https://pdfs.semanticscholar.org/cc4c/8c969d0faf55276c51e9eb9424d5d77b7749.pdf
Do not over-dry SPRI beads, as this leads to difficult re-hydration and elution. If ... suspend washed Dynabeads in 50 µL of binding buffer.
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78 PCR purification with ASSIST PLUS pipetting robot | INTEGRA
https://www.integra-biosciences.com/united-states/en/applications/pcr-purification-beckman-coulter-ampure-xp-magnetic-beads-and-assist-plus
Agencourt AMPure XP beads (Beckman Coulter) are used for DNA purification in a variety of applications, including PCR, NGS, cloning and microarrays.
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79 General Schedule
https://www.um.edu.mt/__data/assets/pdf_file/0005/358097/TrainMalta_2018_Practicals_2018_07_13.pdf
Take a 300 μl PCR tube containing 50 μl Protein-A Magnetic Beads ... The different Ampure XP ratios can be used to bind for different molecular weights, ...
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80 MagPure A4 XP BP - Magen Biotechnology
https://www.magen-tec.com/products/info.aspx?itemid=394
AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can ...
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81 Xpert Purification SPRI Magnetic Beads - Syntezza Bioscience
https://www.syntezza.com/product/xpert-purification-spri-magnetic-beads/
The magnetic beads are supplied in a buffer that has been optimized in order to selectively bind DNA fragments of 100bp and larger. Primers, primer dimers, ...
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82 Bioanalysis - Cultek
https://www.cultek.com/media/wysiwyg/catalogos/MACHEREY-NAGEL/clean_up_size_selection.pdf
The magnetic beads in the kit are present in binding buffer ... DNA PCR Free kit and. Illumina (red), AMPure® XP (blue) and NucleoMag® NGS Clean-up and Size.
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83 CUT&RUN.salt protocol1 - Genes Dev
http://genesdev.cshlp.org/content/suppl/2018/01/31/gad.307736.117.DC1/Supplemental_Material.pdf
10) Optional Ampure bead selection (bead pellet fraction only) ... vicinity of a DNA-binding protein, but this can vary depending on the steric access to ...
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84 MinION PROTOCOL - Andersen Lab
https://andersenlab.org/Protocols/MinION_R7.3.pdf
After the two AMPure bead cleanup steps that puts ... Allow DNA to bind to beads by rotating for 5 minutes on a nutator.
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85 We adopted the protocol described in the Extended - encode
https://www.encodeproject.org/documents/0dd3624f-d1f9-4505-b97c-da01b90a70f4/@@download/attachment/Hi-C%20Protocol.pdf
To increase yield, AMPure XP beads can be concentrated by removing some of the ... with rotation to bind biotinylated DNA ​ ​to the streptavidin beads. ​.
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86 Split & mix assembly of DNA libraries for ultrahigh throughput ...
https://academic.oup.com/nar/article/48/11/e63/5834577
Beads are split into different tubes, with the number of vessels corresponding to the desired number of different amino acid variants at a position of interest ...
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87 Keats Lab - AMPure and not-so-simple - keatslab.org
https://www.keatslab.org/blog/ampureandnot-so-simple
Interesting, I thought. You can clearly see that as the bead:sample ratio decreases, the beads selectively bind larger fragment sizes. A nice ...
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88 Magnetic beads for dna purification - Sigma-Aldrich
https://www.sigmaaldrich.com/CH/en/search/magnetic-beads-for-dna-purification?focus=documents&page=1&perpage=30&sort=relevance&term=magnetic%20beads%20for%20dna%20purification&type=site_content
if DNA purification is required for your analysis. 1. Gently shake the Agencourt AMPure XP DNA purification beads bottle to resuspend ...
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89 ILLUMINA WORKFLOW PART-2 (DNA TAGMENTATION ...
https://upcvmda-pl480.weebly.com/educational-articles/dna-sequencing-illumina-workflow-part-2-dna-tagmentation-amplification-and-cleanup
When beads are added to the Index PCR product in the presence of the crowding agent (usually in the bead buffer), the PEG causes the negatively- ...
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90 Pcr Beads at Thomas Scientific
https://www.thomassci.com/scientific-supplies/Pcr-Beads
…designed to recover highly concentrated DNA from PCR products. It is suitable for automated purification of PCR samples. By using the highly efficient binding ...
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91 HigherPurity™ PCR Clean-Up Magnetic Beads - 5 mL
https://www.youdobio.com/product/higherpurity-dna-purification-spri-magnetic-beads/
The Magnetic beads are pre‐formulated with an optimized buffer to selectively bind DNA fragments of 100 bp and larger. Excess salts, enzymes, primers and ...
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92 NGS Clean and Select Beads - Meridian Bioscience
https://www.meridianbioscience.com/lifescience/products/molecular-reagents/next-generation-sequencing/ngs-clean-and-select-beads/
Paramagnetic beads selectively bind DNA fragments based on the volume ratio of bead suspension and sample. They are capable of delivering highly purified DNA ...
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93 sparQ Pure Mag Beads - Fast, reliable DNA purification & size ...
https://www.genetargetsolutions.com.au/product/sparq-pure-mag-beads/
Based on the reversible nucleic acid-binding properties of magnetic beads, this product can be used to quickly remove primers, primer-dimers ...
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94 Magnetic Beads for DNA and RNA Purification - Nucleus Biotech
https://nucleusbiotech.com/produkt/magnetic-beads-for-dna-and-rna-purification-dna-100-bp-rna-200-nt-2/
The DNA and RNA beads are optimized to selectively bind DNA fragments of 100 bp and larger, and RNA fragments of 200 bases and larger. Purified DNA and RNA ...
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