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Google Keyword Rankings for : how does peg concentrate virus
1
Concentration of infectious SARS-CoV-2 by polyethylene ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510776/
We have described the use of Polyethylene glycol (PEG) for the precipitation of natural communities of aquatic viruses, and its comparison with the usual ...
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510776/
We have described the use of Polyethylene glycol (PEG) for the precipitation of natural communities of aquatic viruses, and its comparison with the usual ...
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2
Homemade 4-fold lentivirus concentrator
https://www.mdanderson.org/documents/core-facilities/Functional%20Genomics%20Core/Homemade%204fold%20lentivirus%20concentrator.pdf
The concentrations of PEG-8000 and NaCl in the stock solution are 40% (W/V) and. 1.2M, respectively. Store the solution at 4 oC. Virus concentration ...
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https://www.mdanderson.org/documents/core-facilities/Functional%20Genomics%20Core/Homemade%204fold%20lentivirus%20concentrator.pdf
The concentrations of PEG-8000 and NaCl in the stock solution are 40% (W/V) and. 1.2M, respectively. Store the solution at 4 oC. Virus concentration ...
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3
PEG Virus Precipitation Kit (MAK343) - Sigma-Aldrich
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/489/311/mak343bul.pdf
The concentrated virus can be used for infection, viral DNA, or RNA purification, etc. Components. The kit is sufficient for 200 preparations. PEG Solution ...
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https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/489/311/mak343bul.pdf
The concentrated virus can be used for infection, viral DNA, or RNA purification, etc. Components. The kit is sufficient for 200 preparations. PEG Solution ...
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4
Comparison of five polyethylene glycol precipitation ...
https://www.sciencedirect.com/science/article/pii/S0048969721058009
Highlights. •. Polyethylene glycol (PEG)-mediated precipitation is an established methodology for concentration of virus stocks and is applied here to SARS ...
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https://www.sciencedirect.com/science/article/pii/S0048969721058009
Highlights. •. Polyethylene glycol (PEG)-mediated precipitation is an established methodology for concentration of virus stocks and is applied here to SARS ...
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5
PEG-it Virus Precipitation Solution - System Biosciences
https://www.systembio.com/products/lentivirus-production/virus-concentration-and-titering/peg-it-virus-precipitation-solution
Easily concentrate lentivirus with an ultracentrifugation-free protocol—great for scaled-up virus production and also protects virus from freeze-thaw ...
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https://www.systembio.com/products/lentivirus-production/virus-concentration-and-titering/peg-it-virus-precipitation-solution
Easily concentrate lentivirus with an ultracentrifugation-free protocol—great for scaled-up virus production and also protects virus from freeze-thaw ...
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6
PEG Virus Precipitation Kit (ab102538/K904-50) - Abcam
https://www.abcam.com/peg-virus-precipitation-kit-ab102538.html
Abcam's PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate viruses without ultra-centrifugation. The kit can be ...
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https://www.abcam.com/peg-virus-precipitation-kit-ab102538.html
Abcam's PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate viruses without ultra-centrifugation. The kit can be ...
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7
Protocol for Concentrating RNA Viruses by PEG Precipitation
https://www.epigentek.com/catalog/protocol-for-concentrating-rna-viruses-by-peg-precipitation-n-48.html?newsPath=1
Precipitation methods have been developed to rapidly, easily, and cheaply concentrate viruses and remove impurities on the basis of solubility in solutions of ...
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https://www.epigentek.com/catalog/protocol-for-concentrating-rna-viruses-by-peg-precipitation-n-48.html?newsPath=1
Precipitation methods have been developed to rapidly, easily, and cheaply concentrate viruses and remove impurities on the basis of solubility in solutions of ...
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8
Polyethylene glycol (PEG) methods are superior to ... - bioRxiv
https://www.biorxiv.org/content/10.1101/2021.11.19.469352v1.full
Standard methods for the detection of enteric viruses and surrogates that involve acidification could be modified with PEG precipitation to ...
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https://www.biorxiv.org/content/10.1101/2021.11.19.469352v1.full
Standard methods for the detection of enteric viruses and surrogates that involve acidification could be modified with PEG precipitation to ...
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9
Concentration and purification of viruses and bacteriophages ...
https://link.springer.com/content/pdf/10.1007/BF02876605.pdf
McClendon (1954) was probably the first to use polyethylene glycol (PEG) for precipitating a fraction of a suspension. Working with isolated chloroplast suspen-.
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https://link.springer.com/content/pdf/10.1007/BF02876605.pdf
McClendon (1954) was probably the first to use polyethylene glycol (PEG) for precipitating a fraction of a suspension. Working with isolated chloroplast suspen-.
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10
Concentrating virus (PEG) - Cell Biology Protocols
http://www.sciencegateway.org/protocols/cellbio/proteine/cv.htm
1. Viruses such as baculovirus can be concentrated by polyethylene glycol precipitation. 2. Adjust the pH of the virus-containing supernatant to 7.2-7.5.
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http://www.sciencegateway.org/protocols/cellbio/proteine/cv.htm
1. Viruses such as baculovirus can be concentrated by polyethylene glycol precipitation. 2. Adjust the pH of the virus-containing supernatant to 7.2-7.5.
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11
ExtraPEG: A Polyethylene Glycol-Based Method for ... - Nature
https://www.nature.com/articles/srep23978
Initially thought to be a means for cells to eliminate waste, secreted extracellular vesicles, known as exosomes, are now understood to ...
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https://www.nature.com/articles/srep23978
Initially thought to be a means for cells to eliminate waste, secreted extracellular vesicles, known as exosomes, are now understood to ...
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12
Concentrate Lentivirus with PEG8000 precipitation
https://hanlab.cc/list.php?ID=41
1. Prepare 4x PEG8000/NaCl solution: Dissolve 80g PEG-8000, 14.0g NaCl in 80ml MillQ water and 20ml of 10x PBS (pH7.
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https://hanlab.cc/list.php?ID=41
1. Prepare 4x PEG8000/NaCl solution: Dissolve 80g PEG-8000, 14.0g NaCl in 80ml MillQ water and 20ml of 10x PBS (pH7.
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13
Polyethylene glycol precipitation for recovery of pathogenic ...
https://journals.asm.org/doi/pdf/10.1128/aem.54.8.1983-1988.1988
Polyethylene glycol 6000 precipitation was found to be an effective concentration method that enhanced the chances for detecting human virus ...
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https://journals.asm.org/doi/pdf/10.1128/aem.54.8.1983-1988.1988
Polyethylene glycol 6000 precipitation was found to be an effective concentration method that enhanced the chances for detecting human virus ...
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14
PEG-it Virus Precipitation Solution (100 mL aliquot)
https://www1.biocat.com/products/LV810A-1-SBI
Easy to use and cost-effective method for highly efficient viral concentration · Concentrate all lentiviral particles 10- to 100-fold with PEG-it™ · Freeze ...
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https://www1.biocat.com/products/LV810A-1-SBI
Easy to use and cost-effective method for highly efficient viral concentration · Concentrate all lentiviral particles 10- to 100-fold with PEG-it™ · Freeze ...
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15
How do I optimise PEG precipitation? - ResearchGate
https://www.researchgate.net/post/How_do_I_optimise_PEG_precipitation
I am trying to purify a heterologously-produced virus-like particle, and PEG precipitation (or ammonium sulfate precipitation) would really help.
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https://www.researchgate.net/post/How_do_I_optimise_PEG_precipitation
I am trying to purify a heterologously-produced virus-like particle, and PEG precipitation (or ammonium sulfate precipitation) would really help.
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16
A Simple and Effective Method to Concentrate Hepatitis C Virus
https://www.mdpi.com/1999-4915/14/9/1987/pdf
Moreover, a large number of infectious viruses are lost during enrichment. We developed a new method of hepatitis C virus (HCV) concentration to ...
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https://www.mdpi.com/1999-4915/14/9/1987/pdf
Moreover, a large number of infectious viruses are lost during enrichment. We developed a new method of hepatitis C virus (HCV) concentration to ...
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17
Viro-PEG Lentivirus Concentrator OZ Biosciences Protocol
https://fnkprddata.blob.core.windows.net/domestic/data/datasheet/OZB/LVG500.pdf
For high concentration virus, the resuspension volume should be limited between 1/10th and 1/100th of the original sample. • Depending on the initial viral ...
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https://fnkprddata.blob.core.windows.net/domestic/data/datasheet/OZB/LVG500.pdf
For high concentration virus, the resuspension volume should be limited between 1/10th and 1/100th of the original sample. • Depending on the initial viral ...
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18
Concentration of viruses from wastewater influent using ...
https://www.protocols.io/view/concentration-of-viruses-from-wastewater-influent-bi6jkhcn
› view › concentration-of-viru...
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https://www.protocols.io/view/concentration-of-viruses-from-wastewater-influent-bi6jkhcn
› view › concentration-of-viru...
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19
PEG Virus Precipitation Kit - BioVision
https://www.biovision.com/documentation/datasheets/K904.pdf
The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc. II. Kit Contents: III.
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https://www.biovision.com/documentation/datasheets/K904.pdf
The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc. II. Kit Contents: III.
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20
PEG Virus Precipitation Kit | High Viral Titer, No ... - APExBIO
https://www.apexbt.com/peg-virus-precipitation-kit.html
The PEG Virus Precipitation Kit offers a fast and convenient way for concentrating virus without ultra-centrifugation. The kit can be utilized in both small ...
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https://www.apexbt.com/peg-virus-precipitation-kit.html
The PEG Virus Precipitation Kit offers a fast and convenient way for concentrating virus without ultra-centrifugation. The kit can be utilized in both small ...
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21
Lentivirus concentration protocol - Broad Institute
https://portals.broadinstitute.org/gpp/public/dir/download?dirpath=protocols/production&filename=Virus_Concentration_Protocol_feb2018.pdf
unconcentrated viral supernatant have been obtained. Materials & Instrumentation. • Centrifuge with swinging buckets able to reach 4,000 g that can maintain ...
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https://portals.broadinstitute.org/gpp/public/dir/download?dirpath=protocols/production&filename=Virus_Concentration_Protocol_feb2018.pdf
unconcentrated viral supernatant have been obtained. Materials & Instrumentation. • Centrifuge with swinging buckets able to reach 4,000 g that can maintain ...
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22
PEG-it™ Lentivirus Concentration - Stratech
https://www.stratech.co.uk/wp-content/uploads/2016/10/Joint-Branded-PEG-it-product-sheet-1.pdf
PEG-it efficiently precipitates lentivirus and cryoprotects viral particles during long-term storage and even several freeze/thaw cycles. The procedure is ...
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https://www.stratech.co.uk/wp-content/uploads/2016/10/Joint-Branded-PEG-it-product-sheet-1.pdf
PEG-it efficiently precipitates lentivirus and cryoprotects viral particles during long-term storage and even several freeze/thaw cycles. The procedure is ...
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23
Lentiviral concentration and precipitation: PEG-it
https://www.bioscience.co.uk/cpl/lentiviral-concentration-precipitation-peg-it
PEG-it™ virus precipitation solution from System Biosciences is an effective and easy-to-use reagent for highly efficient viral concentration.
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https://www.bioscience.co.uk/cpl/lentiviral-concentration-precipitation-peg-it
PEG-it™ virus precipitation solution from System Biosciences is an effective and easy-to-use reagent for highly efficient viral concentration.
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24
Viro-PEG - Lentivirus Concentration without ultracentrifugation
https://ozbiosciences.com/lentivirus-retrovirus/114-viro-peg-lentivirus-concentrator.html
The Viro-PEG Lentivirus Concentrator is a ready-to-use solution optimized for the capture and concentration of lentiviral particles, providing an easy and ...
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https://ozbiosciences.com/lentivirus-retrovirus/114-viro-peg-lentivirus-concentrator.html
The Viro-PEG Lentivirus Concentrator is a ready-to-use solution optimized for the capture and concentration of lentiviral particles, providing an easy and ...
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25
WinterVomitingLab:Protocols/PEG precipitation - OpenWetWare
https://openwetware.org/wiki/WinterVomitingLab:Protocols/PEG_precipitation
Non-enveloped virus concentration using Polyethylene Glycol (PEG) precipitation. Materials. Polyethylene Glycol with MW of 6,000 to 10,000; NaCl ...
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https://openwetware.org/wiki/WinterVomitingLab:Protocols/PEG_precipitation
Non-enveloped virus concentration using Polyethylene Glycol (PEG) precipitation. Materials. Polyethylene Glycol with MW of 6,000 to 10,000; NaCl ...
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26
Evaluation of Methods for the Concentration and Extraction of ...
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0170199
Viral sewage metagenomics is a novel field of study used for surveillance, epidemiological studies, and evaluation of waste water treatment ...
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https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0170199
Viral sewage metagenomics is a novel field of study used for surveillance, epidemiological studies, and evaluation of waste water treatment ...
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27
Sewage concentration (PEG method) - Compare Europe
https://www.compare-europe.eu/-/media/sites/compare-europe/protocols/lop_sewage-peg_compare_ifremer_v1.pdf?la=da&hash=895148D4125C5F5C6EBFFBF3C6E3C6D8777476D4
Sewage concentration. (PEG ... Sewages viral concentration using PEG method ... method is used to elute and concentrate enteric viruses from.
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https://www.compare-europe.eu/-/media/sites/compare-europe/protocols/lop_sewage-peg_compare_ifremer_v1.pdf?la=da&hash=895148D4125C5F5C6EBFFBF3C6E3C6D8777476D4
Sewage concentration. (PEG ... Sewages viral concentration using PEG method ... method is used to elute and concentrate enteric viruses from.
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28
Efficiency of PEG secondary concentration and PCR for the ...
https://academic.oup.com/femsle/article/367/10/fnaa085/5841523
Fresh fruits are a potential source of many different pathogens, including bacteria, enteric viruses and protozoa that may pose serious health ...
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https://academic.oup.com/femsle/article/367/10/fnaa085/5841523
Fresh fruits are a potential source of many different pathogens, including bacteria, enteric viruses and protozoa that may pose serious health ...
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29
Peg It Virus Precipitation Solution Protocol
https://techhubsouthflorida.org/wp-content/uploads/formidable/30/peg-it-virus-precipitation-solution-protocol.pdf
While gradient purification is useful, which will not directly affect the tropism of lentivirus. Pathogenesis of gut virus infection.
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https://techhubsouthflorida.org/wp-content/uploads/formidable/30/peg-it-virus-precipitation-solution-protocol.pdf
While gradient purification is useful, which will not directly affect the tropism of lentivirus. Pathogenesis of gut virus infection.
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30
757. Gene Transfer to Human Hematopoietic Cells with GaLV ...
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(16)36561-3
Pseudotyped retroviruses packaged in the envelope derived from gibbon ape leukemia virus (GaLV) show the high transduction efficiency of human hematopoietic ...
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https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(16)36561-3
Pseudotyped retroviruses packaged in the envelope derived from gibbon ape leukemia virus (GaLV) show the high transduction efficiency of human hematopoietic ...
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31
Producing and Purifying Viral Systems in the Laboratory
https://www.youtube.com/watch?v=Yc7CLOviOPk
Production and purification of viral systems are an essential part of laboratory research, whether at a small, academic scale, or a larger, ...
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https://www.youtube.com/watch?v=Yc7CLOviOPk
Production and purification of viral systems are an essential part of laboratory research, whether at a small, academic scale, or a larger, ...
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32
Concentrate lentiviral supernatants without ultracentrifugation
https://www.takarabio.com/products/gene-function/viral-transduction/lentivirus/lentivirus-concentration
In the simple protocol, you just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in ...
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https://www.takarabio.com/products/gene-function/viral-transduction/lentivirus/lentivirus-concentration
In the simple protocol, you just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in ...
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33
Concentration and Quantification of SARS-CoV-2 RNA in ...
https://pdfs.semanticscholar.org/116b/234fdbf3a2c6b44532ea70f70e72f30b888b.pdf
PCR (qRT-PCR) for viral detection in wastewater. PEG-based concentration of viruses is a simple procedure which is not limited by the ...
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https://pdfs.semanticscholar.org/116b/234fdbf3a2c6b44532ea70f70e72f30b888b.pdf
PCR (qRT-PCR) for viral detection in wastewater. PEG-based concentration of viruses is a simple procedure which is not limited by the ...
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34
Production, concentration and purification strategies for ...
https://fenix.tecnico.ulisboa.pt/downloadFile/563345090414947/Dissertacao%20-%20Sonia%20Alves.pdf
The purification of virus particles with removal of impurities was achieved by a combination of different unit operations, which included precipitation,.
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https://fenix.tecnico.ulisboa.pt/downloadFile/563345090414947/Dissertacao%20-%20Sonia%20Alves.pdf
The purification of virus particles with removal of impurities was achieved by a combination of different unit operations, which included precipitation,.
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35
PEG-it™ Virus Precipitation Solution, System Biosciences - VWR
https://us.vwr.com/store/product/25751342/peg-ittm-virus-precipitation-solution-system-biosciences
PEG-it™ virus concentration reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers.Immunological assays are a ...
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https://us.vwr.com/store/product/25751342/peg-ittm-virus-precipitation-solution-system-biosciences
PEG-it™ virus concentration reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers.Immunological assays are a ...
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36
Concentration and purification of enterovirus 71 using a weak ...
https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-11-99
EV-71 viral particles of up to 95% purity can be recovered by a single step ion-exchange chromatography using CIM-DEAE monolithic columns and 1 ...
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https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-11-99
EV-71 viral particles of up to 95% purity can be recovered by a single step ion-exchange chromatography using CIM-DEAE monolithic columns and 1 ...
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37
Single-Use Capture Purification of Adeno-Associated Viral ...
https://www.liebertpub.com/doi/pdf/10.1089/hum.2019.284
We present membrane-based steric exclusion chromatography (SXC) as a universal capture step for purification of adeno- associated virus ...
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https://www.liebertpub.com/doi/pdf/10.1089/hum.2019.284
We present membrane-based steric exclusion chromatography (SXC) as a universal capture step for purification of adeno- associated virus ...
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38
Comparison of virus concentration methods for the RT-qPCR ...
https://research.bangor.ac.uk/portal/files/29192587/untitled.pdf
Murine hepatitis virus (MHV) is an enveloped and positive-sense single-stranded RNA Betacoronavirus, which belongs to the same genus as SARS-CoV ...
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https://research.bangor.ac.uk/portal/files/29192587/untitled.pdf
Murine hepatitis virus (MHV) is an enveloped and positive-sense single-stranded RNA Betacoronavirus, which belongs to the same genus as SARS-CoV ...
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39
Lentivirus Concentrator - Gentarget
https://www.gentarget.com/product/lentivirus-concentrator/
Polymeric precipitation reagents allow for the precipitation of nanosized virus particle at low speed centrifugation. It breaks down the charges around viral ...
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https://www.gentarget.com/product/lentivirus-concentrator/
Polymeric precipitation reagents allow for the precipitation of nanosized virus particle at low speed centrifugation. It breaks down the charges around viral ...
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40
PEG didn't precipitate right away....what is your experience or ...
http://www.protocol-online.org/biology-forums/posts/40049.html
I am trying to concentrate phages from an environmental freshwater sample by PEG precipitation. Unfortunately it doesn't work as I would have ...
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http://www.protocol-online.org/biology-forums/posts/40049.html
I am trying to concentrate phages from an environmental freshwater sample by PEG precipitation. Unfortunately it doesn't work as I would have ...
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41
Journal of Extracellular Vesicles - Taylor and Francis
https://www.tandfonline.com/doi/full/10.1080/20013078.2018.1528109
Several techniques are available to harvest EVs from various body fluids or cell culture supernatants. Classically, differential centrifugation, ...
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https://www.tandfonline.com/doi/full/10.1080/20013078.2018.1528109
Several techniques are available to harvest EVs from various body fluids or cell culture supernatants. Classically, differential centrifugation, ...
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42
Comparison of virus concentration methods for the RT-qPCR
https://digitalcommons.usf.edu/cgi/viewcontent.cgi?article=1001&context=usf_fcrc_all
Murine hepatitis virus (MHV) is an enveloped and positive-sense single-stranded RNA Betacoronavirus, which belongs to the same genus as SARS-CoV ...
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https://digitalcommons.usf.edu/cgi/viewcontent.cgi?article=1001&context=usf_fcrc_all
Murine hepatitis virus (MHV) is an enveloped and positive-sense single-stranded RNA Betacoronavirus, which belongs to the same genus as SARS-CoV ...
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43
Polyethylene glycol purification of influenza virus with ... - JSTOR
https://www.jstor.org/stable/3861421
P.E.G. at appreciably lower polymer concentration than found by Kanarek & Tribe. (1967). As high concentrations of the polymer in influenza vaccines would ...
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https://www.jstor.org/stable/3861421
P.E.G. at appreciably lower polymer concentration than found by Kanarek & Tribe. (1967). As high concentrations of the polymer in influenza vaccines would ...
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44
Low concentration of polyethylene glycol facilitates separation ...
https://journals.physiology.org/doi/full/10.1152/ajplung.00318.2020
by H Lee · 2021 · Cited by 1 —
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https://journals.physiology.org/doi/full/10.1152/ajplung.00318.2020
by H Lee · 2021 · Cited by 1 —
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45
A Review of the Methods for Concentrating M13 Phage
http://www.biotechrep.ir/article_105916_6f8e7f329994375366ff479f9377444d.pdf
Keywords: M13 Bacteriophage, Phage Concentration, Phage Purification, Phage Display, Phage Precipitation. Citation: Fouladvand F, Bemani P, ...
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http://www.biotechrep.ir/article_105916_6f8e7f329994375366ff479f9377444d.pdf
Keywords: M13 Bacteriophage, Phage Concentration, Phage Purification, Phage Display, Phage Precipitation. Citation: Fouladvand F, Bemani P, ...
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46
PEG conjugation moderately protects adeno-associated viral ...
http://www.cchem.berkeley.edu/schaffer/2005%20Publications/Lee_AAVPEG_BB_05.pdf
In addition to adenoviral vectors, PEGylation of vesicular stomatitis virus G protein pseudotyped lentiviral vector pro- vided a 20-fold resistance to antiserum ...
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http://www.cchem.berkeley.edu/schaffer/2005%20Publications/Lee_AAVPEG_BB_05.pdf
In addition to adenoviral vectors, PEGylation of vesicular stomatitis virus G protein pseudotyped lentiviral vector pro- vided a 20-fold resistance to antiserum ...
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47
Virus Purification Methods - News Medical
https://www.news-medical.net/life-sciences/Virus-Purification-Methods.aspx
There are several ways of purifying viruses. They are outlined below. Ultracentrifugation. Ultracentrifugation is used to separate macromolecules into ...
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https://www.news-medical.net/life-sciences/Virus-Purification-Methods.aspx
There are several ways of purifying viruses. They are outlined below. Ultracentrifugation. Ultracentrifugation is used to separate macromolecules into ...
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48
Precipitation with polyethylene glycol followed by washing ...
https://pure.mpg.de/rest/items/item_3038779_4/component/file_3038801/content
We show that washing of the PEG pellet and the re-precipitation by ultracentrifugation remove a huge proportion of PEG co-precipi- tated ...
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https://pure.mpg.de/rest/items/item_3038779_4/component/file_3038801/content
We show that washing of the PEG pellet and the re-precipitation by ultracentrifugation remove a huge proportion of PEG co-precipi- tated ...
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49
Lentiviral Vector Preparation Application Note
https://media.beckman.com/-/media/pdf-assets/application-notes/centrifuge-application-note-lentiviral-vector-preparation-ultra.pdf
Viral vectors are one of the most commonly used tools for genetic manipulation in mammalian cells. They are used both for gene expression as well as gene ...
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https://media.beckman.com/-/media/pdf-assets/application-notes/centrifuge-application-note-lentiviral-vector-preparation-ultra.pdf
Viral vectors are one of the most commonly used tools for genetic manipulation in mammalian cells. They are used both for gene expression as well as gene ...
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50
An improved method for concentrating rotavirus from water ...
https://www.scielo.br/j/mioc/a/XLcvdTfWYny6Ddyky74bRYF/?lang=en
Reconcentration of water samples by a speedVac concentrator showed significantly higher rotavirus recovery than polyethylene glycol precipitation through both ...
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https://www.scielo.br/j/mioc/a/XLcvdTfWYny6Ddyky74bRYF/?lang=en
Reconcentration of water samples by a speedVac concentrator showed significantly higher rotavirus recovery than polyethylene glycol precipitation through both ...
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51
Method for simple and rapid concentration of Zika virus ...
https://hal.archives-ouvertes.fr/hal-01789248/document
The use of centrifugal filter devices to increase infectious titers of ZIKV from cell- culture supernatants is highlighted here. A mean gain of ...
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https://hal.archives-ouvertes.fr/hal-01789248/document
The use of centrifugal filter devices to increase infectious titers of ZIKV from cell- culture supernatants is highlighted here. A mean gain of ...
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52
Purification of viruses by centrifugation - SOEST Hawaii
http://www.soest.hawaii.edu/oceanography/faculty/steward/StewardLab/Projects_files/Lawrence&Steward2010_MAVE.pdf
This method eliminates pelleting and aggrega- tion problems with virus applications, and produces highly. Page 3. Lawrence and Steward. Viral purification by ...
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http://www.soest.hawaii.edu/oceanography/faculty/steward/StewardLab/Projects_files/Lawrence&Steward2010_MAVE.pdf
This method eliminates pelleting and aggrega- tion problems with virus applications, and produces highly. Page 3. Lawrence and Steward. Viral purification by ...
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53
Purification and Electron Cryomicroscopy of Coronavirus ...
https://escholarship.org/content/qt7np5x9hf/qt7np5x9hf_noSplash_603f4783aaf1e9307563ba6f34fde19e.pdf
Electron microscopy (EM) overcomes some problems associated with particle variability and has been an important tool for investigating coronavirus ...
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https://escholarship.org/content/qt7np5x9hf/qt7np5x9hf_noSplash_603f4783aaf1e9307563ba6f34fde19e.pdf
Electron microscopy (EM) overcomes some problems associated with particle variability and has been an important tool for investigating coronavirus ...
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54
LentiFuge™ Viral Concentration Reagent | Cellecta, Inc
https://cellecta.com/products/lentifuge-viral-concentration-reagent
LentiFuge™ viral concentration provides a simple, rapid, and effective method to increase the amount of lentiviral particles collected from cell culture ...
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https://cellecta.com/products/lentifuge-viral-concentration-reagent
LentiFuge™ viral concentration provides a simple, rapid, and effective method to increase the amount of lentiviral particles collected from cell culture ...
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55
Intact Virus Precipitation Reagent (optimized for SARS-CoV-2)
https://www.thermofisher.com/order/catalog/product/10720D
The isolated intact virus can be used for functional studies, in contrast to current SARS-CoV-2 purification methods based on extracting RNA for detection ...
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https://www.thermofisher.com/order/catalog/product/10720D
The isolated intact virus can be used for functional studies, in contrast to current SARS-CoV-2 purification methods based on extracting RNA for detection ...
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56
Virus purification, detection and removal
https://digitalcommons.mtu.edu/cgi/viewcontent.cgi?article=1851&context=etds
PEG and salts can purify virus by altering its solubility causing precipitation or salting out effect [16, 17]. An alternate concept has.
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https://digitalcommons.mtu.edu/cgi/viewcontent.cgi?article=1851&context=etds
PEG and salts can purify virus by altering its solubility causing precipitation or salting out effect [16, 17]. An alternate concept has.
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57
Small-Scale Preparation of Bacteriophage by PEG Precipitation
http://www.abdesignlabs.com/technical-resources/bacteriophage-preparation/
› Technical Resources
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http://www.abdesignlabs.com/technical-resources/bacteriophage-preparation/
› Technical Resources
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58
Concentration and Purification of Viruses by Ultrafiltration
https://www.sartorius.com/download/1160324/concentration-and-purification-viruses-application-guide-en-3--data.pdf
structure of the virus.17 Thus ultrafiltration is very well suited to isolate and concentrate virus particles from water.
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https://www.sartorius.com/download/1160324/concentration-and-purification-viruses-application-guide-en-3--data.pdf
structure of the virus.17 Thus ultrafiltration is very well suited to isolate and concentrate virus particles from water.
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59
Precipitation of Bacteriophage λ Particles from Large-scale ...
http://cshprotocols.cshlp.org/content/2006/1/pdb.prot3966.full
INTRODUCTION. Bacteriophage λ particles are recovered from bacterial lysates by precipitation with polyethylene glycol at high ionic strength.
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http://cshprotocols.cshlp.org/content/2006/1/pdb.prot3966.full
INTRODUCTION. Bacteriophage λ particles are recovered from bacterial lysates by precipitation with polyethylene glycol at high ionic strength.
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60
SARS-CoV-2 detection in sewage samples
https://journals.lww.com/ijmr/Fulltext/2021/01000/SARS_CoV_2_detection_in_sewage_samples_.13.aspx
RNA extraction and real-time RT-PCR: Viral RNA was extracted from the clarified sewage sample and the three phases using QIAamp Viral RNA Mini Kit (QIAGEN, ...
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https://journals.lww.com/ijmr/Fulltext/2021/01000/SARS_CoV_2_detection_in_sewage_samples_.13.aspx
RNA extraction and real-time RT-PCR: Viral RNA was extracted from the clarified sewage sample and the three phases using QIAamp Viral RNA Mini Kit (QIAGEN, ...
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61
Mechanism of Precipitation of Proteins by Polyethylene Glycols
https://www.jbc.org/article/S0021-9258(18)43240-1/pdf
Polyethylene glycol is a nontoxic water-soluble synthetic polymer which is widely used in chemical and biomedical industries. In spite of its ...
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https://www.jbc.org/article/S0021-9258(18)43240-1/pdf
Polyethylene glycol is a nontoxic water-soluble synthetic polymer which is widely used in chemical and biomedical industries. In spite of its ...
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62
Purification Methods and the Presence of RNA in Virus ... - MDPI
https://mdpi-res.com/d_attachment/viruses/viruses-12-00917/article_deploy/viruses-12-00917.pdf?version=1597999059
4. Do DNA Viruses Package RNAs in Virus Particles? A virus particle acts as an extracellular vehicle to transfer the viral genome, virus-encoded ...
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https://mdpi-res.com/d_attachment/viruses/viruses-12-00917/article_deploy/viruses-12-00917.pdf?version=1597999059
4. Do DNA Viruses Package RNAs in Virus Particles? A virus particle acts as an extracellular vehicle to transfer the viral genome, virus-encoded ...
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63
Quantification of SARS-CoV-2 and cross-assembly ... - medRxiv
https://www.medrxiv.org/content/10.1101/2020.05.21.20109181v1.full.pdf
by H Green · 2020 · Cited by 77 —
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https://www.medrxiv.org/content/10.1101/2020.05.21.20109181v1.full.pdf
by H Green · 2020 · Cited by 77 —
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64
FAQ: Retrovirus Transduction - Cell Biolabs, Inc.
https://www.cellbiolabs.com/faq/viral-expression-faq/retroviral-transduction
A: ViraDuctin™ Retrovirus Transduction Kit is a reagent cocktail that uses technology developed at Harvard Medical School and can increase transduction ...
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https://www.cellbiolabs.com/faq/viral-expression-faq/retroviral-transduction
A: ViraDuctin™ Retrovirus Transduction Kit is a reagent cocktail that uses technology developed at Harvard Medical School and can increase transduction ...
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65
A new chemical complex can rapidly concentrate lentivirus ...
https://europepmc.org/articles/pmc5809650/bin/10616_2017_133_moesm2_esm.docx
by JY Lee · 2018 · Cited by 5 —
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https://europepmc.org/articles/pmc5809650/bin/10616_2017_133_moesm2_esm.docx
by JY Lee · 2018 · Cited by 5 —
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66
AAV Production in HEK293T Cells - Addgene
https://www.addgene.org/protocols/aav-production-hek293-cells/
Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.
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https://www.addgene.org/protocols/aav-production-hek293-cells/
Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.
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67
Integrated Process for Capture and Purification of Virus-Like ...
https://www.frontiersin.org/articles/10.3389/fbioe.2020.00489/full
Virus-like particles (VLPs) are emerging nanoscale protein assemblies applied as prophylactic vaccines and in development as therapeutic ...
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https://www.frontiersin.org/articles/10.3389/fbioe.2020.00489/full
Virus-like particles (VLPs) are emerging nanoscale protein assemblies applied as prophylactic vaccines and in development as therapeutic ...
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68
Lentivirus Production
https://pharm.ucsf.edu/sites/pharm.ucsf.edu/files/xinchen/media-browser/lentivirus_production_y2020_update.pdf
› xinchen › media-browser
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https://pharm.ucsf.edu/sites/pharm.ucsf.edu/files/xinchen/media-browser/lentivirus_production_y2020_update.pdf
› xinchen › media-browser
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69
Sample Concentration Protocol for Wastewater Surveillance ...
https://www.idexx.com/files/sample-concentration-protocol-for-wastewater-surveillance.pdf
For example, the IDEXX Water DNA/RNA Magnetic Bead Kit and SARS-CoV-2 RT-PCR Test can be used with a different concentration method than the example PEG ...
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https://www.idexx.com/files/sample-concentration-protocol-for-wastewater-surveillance.pdf
For example, the IDEXX Water DNA/RNA Magnetic Bead Kit and SARS-CoV-2 RT-PCR Test can be used with a different concentration method than the example PEG ...
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70
What is going wrong with my PEG 6000 concentration of ...
https://www.echemi.com/community/what-is-going-wrong-with-my-peg-6000-concentration-of-lentivirus_mjart2205022017_513.html
› community › what-is-going-...
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https://www.echemi.com/community/what-is-going-wrong-with-my-peg-6000-concentration-of-lentivirus_mjart2205022017_513.html
› community › what-is-going-...
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71
Development of a rapid pre-concentration protocol and a ...
https://pubs.rsc.org/en/content/articlehtml/2022/ew/d1ew00539a
by AL Parra-Guardado · 2022 · Cited by 5 —
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https://pubs.rsc.org/en/content/articlehtml/2022/ew/d1ew00539a
by AL Parra-Guardado · 2022 · Cited by 5 —
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72
Multivalent binding of herpesvirus to living cells is tightly ...
https://www.science.org/doi/10.1126/sciadv.aat1273
Viral infection, initiated by the landing of a virion on a cellular surface, is largely defined by the preliminary interactions established between viral ...
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https://www.science.org/doi/10.1126/sciadv.aat1273
Viral infection, initiated by the landing of a virion on a cellular surface, is largely defined by the preliminary interactions established between viral ...
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73
Virus capture using anionic polymer-coated magnetic beads ...
https://www.spandidos-publications.com/10.3892/ijmm.2012.962/download
The recent incidence of emerging and re-emerging viruses is a serious health concern worldwide. The development of transportation systems, such as air travel, ...
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https://www.spandidos-publications.com/10.3892/ijmm.2012.962/download
The recent incidence of emerging and re-emerging viruses is a serious health concern worldwide. The development of transportation systems, such as air travel, ...
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74
Concentration of Epstein-Barr Virus from Cell Culture Fluids ...
https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-20-3-391?crawler=true&mimetype=application/pdf
Epstein-Barr virus (EBV) is usually obtained from supernatant fluids of cultures of primate lymphoblastoid cells. The isolation of small amounts of virus ...
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https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-20-3-391?crawler=true&mimetype=application/pdf
Epstein-Barr virus (EBV) is usually obtained from supernatant fluids of cultures of primate lymphoblastoid cells. The isolation of small amounts of virus ...
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75
K2190-50 | PEG Virus Precipitation Kit Clinisciences
https://www.clinisciences.com/precipitation-2202/peg-virus-precipitation-kit-182007324.html
The kit can be utilized in both small lab sample and large scale virus preparation. It is suitable for concentrating retroviruses, lentiviruses and ...
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https://www.clinisciences.com/precipitation-2202/peg-virus-precipitation-kit-182007324.html
The kit can be utilized in both small lab sample and large scale virus preparation. It is suitable for concentrating retroviruses, lentiviruses and ...
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76
Mammalian reovirus, a nonfusogenic nonenveloped virus ...
https://www.pnas.org/doi/10.1073/pnas.0605835103
by MA Agosto · 2006 · Cited by 135 —
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https://www.pnas.org/doi/10.1073/pnas.0605835103
by MA Agosto · 2006 · Cited by 135 —
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77
Exosomes and viruses - Cell Guidance Systems
https://www.cellgs.com/blog/exosomes-and-viruses.html
Exosomes are a subtype of Extracellular vesicles (EVs), lipid membrane-enclosed compartments ranging in size from 50- to 1000-nm in diameter ...
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https://www.cellgs.com/blog/exosomes-and-viruses.html
Exosomes are a subtype of Extracellular vesicles (EVs), lipid membrane-enclosed compartments ranging in size from 50- to 1000-nm in diameter ...
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78
Comparison of ultracentrifugation methods for concentration of ...
http://eeb.lu.lv/EEB/201212/EEB_10_Hutornojs.pdf
Here, we provide a comparative study of three ultracentrifugation methods used for alphavirus concentration: 20% sucrose cushion, double (20 and 50%) sucrose ...
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http://eeb.lu.lv/EEB/201212/EEB_10_Hutornojs.pdf
Here, we provide a comparative study of three ultracentrifugation methods used for alphavirus concentration: 20% sucrose cushion, double (20 and 50%) sucrose ...
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79
Pressure-driven release of viral genome into a host nucleus is ...
https://elifesciences.org/articles/47212
Internal DNA pressure of tens of atmospheres inside a herpesvirus capsid powers ejection of the viral genome into a cell nucleus, ...
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https://elifesciences.org/articles/47212
Internal DNA pressure of tens of atmospheres inside a herpesvirus capsid powers ejection of the viral genome into a cell nucleus, ...
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80
Wastewater Surveillance Testing Methods | Water-related Topics
https://www.cdc.gov/healthywater/surveillance/wastewater-surveillance/testing-methods.html
› healthywater › testing-methods
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https://www.cdc.gov/healthywater/surveillance/wastewater-surveillance/testing-methods.html
› healthywater › testing-methods
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81
PEG Precipitation: A Powerful Tool for Monoclonal Antibody ...
https://www.biopharminternational.com/view/peg-precipitation-powerful-tool-monoclonal-antibody-purification
This alternative purification method to chromatography is readily scalable and fits a fully disposable downstream process.
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https://www.biopharminternational.com/view/peg-precipitation-powerful-tool-monoclonal-antibody-purification
This alternative purification method to chromatography is readily scalable and fits a fully disposable downstream process.
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82
Filamentous Viruses Grafted with Thermoresponsive Block ...
https://pubs.acs.org/doi/abs/10.1021/acs.macromol.8b00674
Understanding how attractive interactions among rigid polymers or rodlike particles influence their liquid crystal (LC) phase behavior is of ...
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https://pubs.acs.org/doi/abs/10.1021/acs.macromol.8b00674
Understanding how attractive interactions among rigid polymers or rodlike particles influence their liquid crystal (LC) phase behavior is of ...
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83
System Biosciences PEG-it Virus Precipitation Solution
https://www.integratedsci.com.au/product/peg-it-virus-precipitation-solution.html
Simple and highly effective means to concentrate lentiviral particles, such as those produced with System Biosciences' pPACK Lentivector Packaging System.
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https://www.integratedsci.com.au/product/peg-it-virus-precipitation-solution.html
Simple and highly effective means to concentrate lentiviral particles, such as those produced with System Biosciences' pPACK Lentivector Packaging System.
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84
Purification of Recombinant Vaccinia Virus for Oncolytic and ...
https://discovery.ucl.ac.uk/10038211/1/Final%20Corrected%20Thesis%20D.I.W%20Vincent.pdf
This will not only increase the costs of production but may also result in the requirement for purer material as increasing the amount of viral particles per ...
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https://discovery.ucl.ac.uk/10038211/1/Final%20Corrected%20Thesis%20D.I.W%20Vincent.pdf
This will not only increase the costs of production but may also result in the requirement for purer material as increasing the amount of viral particles per ...
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85
Journal Pre-proof - Digital CSIC
http://digital.csic.es/bitstream/10261/218352/1/oncentration%20methods%20for%20the%20quantification%20of%20coronavirus.pdf
different behaviour during viral concentration, it is recommended to use ... to concentrate enveloped viruses from wastewater samples, PEG ...
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http://digital.csic.es/bitstream/10261/218352/1/oncentration%20methods%20for%20the%20quantification%20of%20coronavirus.pdf
different behaviour during viral concentration, it is recommended to use ... to concentrate enveloped viruses from wastewater samples, PEG ...
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86
MEMBRANE-BASED CONCENTRATION AND RECOVERY ...
https://d.lib.msu.edu/etd/6784/datastream/OBJ/download/MEMBRANE-BASED_CONCENTRATION_AND_RECOVERY_OF_VIRUSES_FROM_COMPLEX_WATER_MATRICES.pdf
achieving high virus recovery and can be designed to efficiently recover viruses even ... centrifugation, PEG precipitation and centrifugal ultrafiltration.
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https://d.lib.msu.edu/etd/6784/datastream/OBJ/download/MEMBRANE-BASED_CONCENTRATION_AND_RECOVERY_OF_VIRUSES_FROM_COMPLEX_WATER_MATRICES.pdf
achieving high virus recovery and can be designed to efficiently recover viruses even ... centrifugation, PEG precipitation and centrifugal ultrafiltration.
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87
Desdouits.pdf - Ifremer
https://littoral.ifremer.fr/content/download/150657/file/Desdouits.pdf
The coastal environment, under increasing anthropogenic pressure, is subjected to contamination by a large number of human viruses from sewage, most of them ...
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https://littoral.ifremer.fr/content/download/150657/file/Desdouits.pdf
The coastal environment, under increasing anthropogenic pressure, is subjected to contamination by a large number of human viruses from sewage, most of them ...
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88
Viral Vectors for Gene Therapy: Methods and Protocols
https://books.google.com/books?id=r2MsxwNvv3IC&pg=PA378&lpg=PA378&dq=how+does+peg+concentrate+virus&source=bl&ots=WLqaTjpfhj&sig=ACfU3U00IiY1mhBs8FhwCrcf5zNRYLhkfQ&hl=en&sa=X&ved=2ahUKEwjl1Z27vMD7AhUQQkEAHVFqDgcQ6AF6BQi0AhAD
For concentrated virus preparation, virus supernatant can be pooled and frozen at ... Concentration by PEG 8000 Precipitation For large volumes of virus ...
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https://books.google.com/books?id=r2MsxwNvv3IC&pg=PA378&lpg=PA378&dq=how+does+peg+concentrate+virus&source=bl&ots=WLqaTjpfhj&sig=ACfU3U00IiY1mhBs8FhwCrcf5zNRYLhkfQ&hl=en&sa=X&ved=2ahUKEwjl1Z27vMD7AhUQQkEAHVFqDgcQ6AF6BQi0AhAD
For concentrated virus preparation, virus supernatant can be pooled and frozen at ... Concentration by PEG 8000 Precipitation For large volumes of virus ...
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89
Guidelines for environmental surveillance of poliovirus ...
https://polioeradication.org/wp-content/uploads/2016/07/WHO_V-B_03.03_eng.pdf
Publications of the World Health Organization can be obtained from Marketing and Dissemination, World Health Organization, 20 Avenue Appia, 1211 Geneva 27, ...
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https://polioeradication.org/wp-content/uploads/2016/07/WHO_V-B_03.03_eng.pdf
Publications of the World Health Organization can be obtained from Marketing and Dissemination, World Health Organization, 20 Avenue Appia, 1211 Geneva 27, ...
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90
Lenti-X Lentiviral Expression Systems User Manual
http://www.takara.co.kr/file/manual/pdf/632586.pdf
increases nuclear importation of the viral genome during target cell infection. The cPPT/CTS element improves vector integration and transduction efficiency ( ...
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http://www.takara.co.kr/file/manual/pdf/632586.pdf
increases nuclear importation of the viral genome during target cell infection. The cPPT/CTS element improves vector integration and transduction efficiency ( ...
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91
Virus Concentration by PEB Precipitation - Kuperwasser Lab
https://kuperwasser-lab.org/virus-concentration-by-peg-precipitation/
Polyethylene glycol, Sigma, MW10000. NaCl, tissue culture grade (?). EDTA, tissue culture grade precipitate (NaCl). H2O, deionized pyrogen free.
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https://kuperwasser-lab.org/virus-concentration-by-peg-precipitation/
Polyethylene glycol, Sigma, MW10000. NaCl, tissue culture grade (?). EDTA, tissue culture grade precipitate (NaCl). H2O, deionized pyrogen free.
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92
Potential Core Inhibitors in Hepatitis B Virus Presented at the ...
https://www.infectioncontroltoday.com/view/potential-core-inhibitors-hepatitis-b-virus-presented-aasld-liver-meeting-
While treatments are available for hepatitis B virus, no long-term cure has been developed. Progress so far indicates that this core ...
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https://www.infectioncontroltoday.com/view/potential-core-inhibitors-hepatitis-b-virus-presented-aasld-liver-meeting-
While treatments are available for hepatitis B virus, no long-term cure has been developed. Progress so far indicates that this core ...
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93
Purification of IgG by Precipitation with Polyethylene Glycol ...
https://experiments.springernature.com/articles/10.1007/978-1-59745-198-7_183
PEG precipitation works well for IgM, but is less efficient for IgG; salt precipitation methods are usually recommended for IgG. PEG precipitation may be ...
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https://experiments.springernature.com/articles/10.1007/978-1-59745-198-7_183
PEG precipitation works well for IgM, but is less efficient for IgG; salt precipitation methods are usually recommended for IgG. PEG precipitation may be ...
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94
QIAamp® Viral RNA Mini Handbook - QIAGEN
https://www.qiagen.com/us/resources/download.aspx?id=c80685c0-4103-49ea-aa72-8989420e3018&lang=en
Under these conditions, QIAamp Mini spin columns and all buffers and reagents can be stored until the expiration date on the kit box, without showing any.
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https://www.qiagen.com/us/resources/download.aspx?id=c80685c0-4103-49ea-aa72-8989420e3018&lang=en
Under these conditions, QIAamp Mini spin columns and all buffers and reagents can be stored until the expiration date on the kit box, without showing any.
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95
Puzzle Games - Math is Fun
https://www.mathsisfun.com/games/puzzle-games.html
› games › puzzle-games
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https://www.mathsisfun.com/games/puzzle-games.html
› games › puzzle-games
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96
What Is Globalization?
https://www.piie.com/microsites/globalization/what-is-globalization
The global economy moves fast. We help you navigate it. The Peterson Institute for International Economics (PIIE) is an independent nonprofit, ...
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https://www.piie.com/microsites/globalization/what-is-globalization
The global economy moves fast. We help you navigate it. The Peterson Institute for International Economics (PIIE) is an independent nonprofit, ...
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