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1 Barry Wanner & Kirill Datsenko - ScienceWatch.com
http://archive.sciencewatch.com/inter/aut/2009/09-apr/09aprWannerET/
Wanner: The method itself is actually a very rapid and efficient method for making mutations. As geneticists who study physiology, one of the key ways in ...
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2 Barry L. Wanner, Ph.D. - Harvard Catalyst Profiles
https://connects.catalyst.harvard.edu/Profiles/profile/35139143
Colony-live--a high-throughput method for measuring microbial colony growth kinetics--reveals diverse growth effects of gene knockouts in Escherichia coli. BMC ...
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3 One-step inactivation of chromosomal genes in ... - PNAS
https://www.pnas.org/doi/10.1073/pnas.120163297
We have developed a simple and highly efficient method to disrupt chromosomal genes in ... Kirill A. Datsenko and Barry L. WannerAuthors Info & Affiliations.
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4 Barry Lee Wanner Harvard Medical School - ResearchGate
https://www.researchgate.net/profile/Barry-Wanner
Barry WANNER, Lecturer | Cited by 38031 | of Harvard Medical School, MA (HMS) | Read ... Comparison of the Colony-live system with the conventional method.
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5 One-step inactivation of chromosomal genes in ... - PubMed
https://pubmed.ncbi.nlm.nih.gov/10829079/
We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the ...
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6 Barry Wanner | Purdue University - Academia.edu
https://purdue.academia.edu/BarryWanner
Barry Wanner, Purdue University, Biological Sciences Department, Faculty Member. ... Use of new methods for construction of tightly regulated arabinose and ...
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7 Regulation of Bacterial Virulence Gene Expression - Barry Wanner
https://grantome.com/grant/NIH/F33-AI010093-01
The regulation of virulence factor gene expression plays a central role in the pathogenesis process. Members of the Mycobacterium fortuitum complex provide ...
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8 Barry L. Wanner | Harvard University | 131 Publications | 33446 ...
https://typeset.io/authors/barry-l-wanner-3n6ywsm3tb
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), ...
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9 One-step inactivation of chromosomal genes in Escherichia ...
https://www.semanticscholar.org/paper/One-step-inactivation-of-chromosomal-genes-in-coli-Datsenko-Wanner/773cded6afb7b47c29d3eae21f7de4ae55babb33
A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers ... K. Datsenko, B. Wanner; Published 6 June 2000 ...
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10 Transcription, processing and function of CRISPR cassettes in ...
https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07265.x
Barry L. Wanner, ... The CRISPR/Cas systems function through small RNAs originating from longer CRISPR cassette transcripts.
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11 High-Throughput Screening of a Promoter Library Reveals ...
https://journals.asm.org/doi/10.1128/spectrum.02253-21
coli BW25113, the derivative of K-12 strain, was generated by the laboratory of Barry L Wanner (73) and used as the parental strain for the Keio ...
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12 One-step inactivation of - Escherichia coli K-12 usir - JSTOR
https://www.jstor.org/stable/122690
Kirill A. Datsenko and Barry L. Wanner*. Department of Biological Sciences, ... method has greatly facilitated the generation of specific mut.
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13 Molecular memory of prior infections activates the CRISPR ...
https://www.nature.com/articles/ncomms1937
Some mutations in foreign DNA that affect the match prevent CRISPR/Cas defensive function. ... Kirill A. Datsenko & Barry L. Wanner.
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14 Efficient Method to Generate Homologous Recombinant ...
https://www.future-science.com/doi/pdf/10.2144/02324st04
vided by Dr. Barry L Wanner) was used for gene replacement experiments. The genetic background of BW25113 is. lacIq rrnBT14 ∆lacZw116 hsdR514.
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15 Use of the rep technique for allele replacement to construct ...
https://www.sciencedirect.com/science/article/pii/0378111994907765/pdf?md5=6208a0d2094c256a804b6b55645b0f10&pid=1-s2.0-0378111994907765-main.pdf&_valck=1
William W. Metcalf, Weihong Jiang and Barry L. Wanner. Department of Biological. Sciences, Purdue University, West Lafayette, IN 47907, USA.
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16 pKD4 (Plasmid #45605) - Addgene
https://www.addgene.org/45605/
Plasmid pKD4 from Dr. Barry L. Wanner's lab contains the insert FRT-kan-FRT and is published in Proc ... Cloning method Unknown; 5′ sequencing primer R6K-3' ...
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17 Construction of Escherichia coli K‐12 in‐frame, single‐gene ...
https://www.embopress.org/doi/10.1038/msb4100050
This breakthrough provided a simple and efficient method for gene ... Crick F (2002) Letter to Barry L. Wanner dated October 1, 2002.
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18 Colony-live — a high-throughput method for measuring ...
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-171
By using a new normalization method for colony growth, Colony-live gives more precise ... Correspondence to Barry L Wanner or Hirotada Mori.
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19 Identification of Essential Genes and Synthetic Lethal Gene ...
https://experiments.springernature.com/articles/10.1007/978-1-4939-2398-4_4
Yuta Otsuka 2 ,. Hitomi Dose 2 ,. Barry L. Wanner Author Email 3. show more details. Series: Methods In Molecular Biology > Book: Gene Essentiality.
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20 Welcome to Microbial Informatics and Experimentation
https://docs.lib.purdue.edu/bioscipubs/10/
... and methods that span diverse microbes have no obvious home. ... This is the publisher pdf of Michael J Wise, Barry L Wanner.
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21 Making Competent Cells expressing Lambda Red - Barrick Lab
https://barricklab.org/twiki/bin/view/Lab/ProcedureGenomeModificationDatsenkoWanner
Methods Mol. Biol. 1279: 45–65. Detailed protocol describing the construction of the Keio collection via the Datsenko & Wanner method.
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22 Asymmetric polar localization dynamics of the serine ... - PLOS
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0195887
Barry L. Wanner , ... The spatial location of proteins in living cells can be critical for their function. For example, the E. coli ...
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23 EcoliWiki: a wiki-based community resource for Escherichia coli
https://academic.oup.com/nar/article/40/D1/D1270/2903473
Note, however, that this method relies on orthologs being annotated as ... Barry Wanner and Paul Thomas, as PIs of the EcoliHub and PortEco ...
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24 Resources for Escherichia coli Systems Biology | SpringerLink
https://link.springer.com/chapter/10.1007/978-1-4020-9394-4_5
This process is experimental and the keywords may be updated as the learning ... Methods Mol Biol 416:183–94 ... Kirill A. Datsenko & Barry L. Wanner.
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25 Ref - Zhou et al. 2004. Methods Mol Biol 267:123-134
https://cgsc.biology.yale.edu/Reference.php?ID=88287
Zhou, L., K. Zhang, B.L. Wanner 2004. Chromosomal expression of foreign and native ... Wanner, Barry, B.L.. Year: 2004; Publication: Methods Mol. Biol.
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26 US20040033608A1 - Plasmids, strains, and methods of use
https://patents.google.com/patent/US20040033608A1/en
Assignors: WANNER, BARRY L., HALDIMANN, ANDREAS: 2004-02-19 Publication of US20040033608A1 publication Critical patent/US20040033608A1/en: 2008-10-24 ...
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27 Wanner, Barry L. Biosketch January 2012 A. Current Position 1993 ...
http://www.anatomy.ncku.edu.tw/files/news/96_894c328a.pdf
Wanner, Barry L. ... WANNER, B. L., R. Kodaira, and F. C. Neidhardt. ... transcriptional fusions and their use with new methods for allele replacement.
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28 Barry Wanner - Lecturer - Harvard Medical School - LinkedIn
https://www.linkedin.com/in/barry-wanner-205214212
View Barry Wanner's profile on LinkedIn, the world's largest professional ... How should project managers determine the best methodology to use in a project ...
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29 Jean-Marc Ghigo - Genetic Methods and Tools - Research
https://research.pasteur.fr/en/tool/genetic-methods-and-tools/
Court (Yu et al., 2000), or Barry Wanner (Datsenko and Wanner, 2000) tools or the plasmids (pKOBEG's) developed in the laboratory.In ...
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30 Construction of Escherichia coli K-12 in ... - WCM-Q SEARCH
https://primo.qatar-weill.cornell.edu/discovery/fulldisplay?docid=cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1681482&context=PC&vid=974WCMCIQ_INST:VU1&lang=en&search_scope=MyInst_and_CI&adaptor=Primo%20Central&tab=Everything&query=creator%2Cexact%2C%20Takai%2C%20Yuki%20%2CAND&facet=creator%2Cexact%2C%20Takai%2C%20Yuki%20&mode=advanced
These mutants were constructed by using a PCR gene replacement method similar ... Wanner, Barry L ; Mori, Hirotada ; Baba, Tomoya ; Ara, Takeshi ; Hasegawa, ...
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31 Development of a system for discovery of genetic interactions ...
https://www.jstage.jst.go.jp/article/ggs/88/4/88_233/_html/-char/en
One method shown to be useful for revealing interactions of essential genes requires ... by one-step homologous recombination (Datsenko and Wanner, 2000).
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32 Catherine Wanner: Barry Director Of The Paterno Fellows ...
https://onwardstate.com/2018/01/30/catherine-wanner-barry-director-of-the-paterno-fellows-program-and-professor-extraordinaire/
Yes, that's correct – the Soviet Union. Wanner made her way into communist territory during her undergraduate days alongside other students, ...
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33 Assembly of Radically Recoded E. coli Genome Segments
https://www.biorxiv.org/content/10.1101/070417v2
... John Aach, Christopher J. Gregg, Barry L. Wanner, George M. Church ... Our method expands the genome engineering toolkit for radical ...
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34 A Multiplex Genome Editing Method for Escherichia coli ...
https://www.frontiersin.org/articles/10.3389/fmicb.2018.02307/full
The method uses a CRISPR-Cas12a system to eliminate unmodified cells by ... We thank Dr. Barry Wanner for providing plasmid pKD46.
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35 ENVIRONMENTAL DEPENDENCY OF GENE KNOCKOUTS ...
https://www.worldscientific.com/doi/pdf/10.1142/S021972001000521X
then adopted our method to the analysis of PM data from selected mutants under ... Barry Wanner is supported by GM092047 from the.
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36 Browsing by Author "Wanner, Barry L."
https://library.naist.jp/dspace/browse?type=author&value=Wanner%2C+Barry+L.&locale-attribute=en
Colony-live --a high-throughput method for measuring microbial colony growth ... Muto, Ai; Wanner, Barry L.; Mori, Hirotada (BioMed Central Ltd, 2014-06-26).
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37 New Directions in Genome Engineering: An Interview with ...
https://systemsbiology.columbia.edu/news/new-directions-in-genome-engineering-an-interview-with-harris-wang
This new method offers a cost-effective way for designing and ... forward occurred when Don Court and Barry Wanner independently developed a ...
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38 Bacterial density dictates virulence in cholera
https://www.cell.com/trends/microbiology/pdf/S0966-842X(02)02455-1.pdf
offer a safe method to treat people ... and should pave the way for trials in children. CK ... by Barry Wanner of Purdue University, will.
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39 Vol. 59, No. 1, 1974 BIOCHEMICAL AND BIOPHYSICAL ...
https://deepblue.lib.umich.edu/bitstream/handle/2027.42/22313/0000757.pdf?sequence=1
MATERIALS AND METHODS. A multiply auxotrophic E. coli strain, JCI553(KLF3-1), which is recombination deficient (recA) was received from Barry Wanner,.
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40 On the influence of cobamides on organohalide respiration ...
https://trace.tennessee.edu/cgi/viewcontent.cgi?article=6963&context=utk_graddiss
function lies in the lower base, and essential component of functional corrinoids. ... Wexler AG, Schofield WB, Degnan PH, Folta-Stogniew E, Barry NA,.
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41 eSGA: E. coli Synthetic Genetic Array analysis
https://protocols.scienceexchange.com/protocols/esga-e-coli-synthetic-genetic-array-analysis
A six-day replica plating method is used to construct double mutants ... Alla G Gagarinova, Oxana Pogoutse, Hirotada Mori, Barry L Wanner, ...
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42 MEGA (Multiple Essential Genes Assembling) Deletion and ...
http://pubs.acs.org/doi/10.1021/sb500324p
We believe that the MEGA deletion and replacement method has potential ... Datsenko, K. A. and Wanner, B. L. (2000) One-step inactivation of ...
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43 List of references - OUCI
https://ouci.dntb.gov.ua/en/works/45Xodj37/
Authors: M R Wilmes-Riesenberg, B L Wanner ... Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion . Proc.
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44 Phenotype MicroArrays for High-Throughput ... - Genome Res
https://genome.cshlp.org/content/11/7/1246.full
Important “global” technologies using two-dimensional methods were pioneered by O'Farrell and ... 1997; Datsenko and Wanner 2000; F. Blattner, pers. comm.
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45 E-GEOD-52059 < ArrayExpress < BioStudies < EMBL-EBI
https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-52059/protocols/
For downloading a higher number of files, please use our alternative downloading methods. Close. Linked Information ... Joe Grissom. Barry Wanner ...
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46 大肠杆菌中遗传相互作用的高通量定量分析。 - X-MOL
https://www.x-mol.com/paper/1431628?adv
Here we describe a method based on F factor-driven conjugation, ... Takeuchi, Rikiya, Wanner, Barry L, Mori, Hirotada, Weissman, Jonathan S, Krogan, ...
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47 Lambda Red-mediated Recombineering in the Attaching and ...
https://jdc.jefferson.edu/cgi/viewcontent.cgi?article=1201&context=medfp
Barry Wanner. pKD13. Template plasmid to amplify FRT-kan-FRT amplicon, AmpR KanR. Barry Wanner. pFT-K flp gene under the control of TetR ...
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48 April 2009 - RRResearch
http://rrresearch.fieldofscience.com/2009/04/
The other strain, BW25113, comes from Barry Wanner by way of the ... The first experiment didn't produce much in the way of results.
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49 Appendix I Clean Deletion of the nth Gene from the ...
https://thesis.library.caltech.edu/6277/9/Appendices.pdf
The methods described here are adapted from those published previously ... obtained from Professor Barry Wanner (Purdue University). The plasmids.
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50 Technique Quickly Identifies Bacteria For Food Safety, Health ...
https://www.sciencedaily.com/releases/2006/12/061228153849.htm
Researchers at Purdue University have used a new technique to ... Ongoing work by Talaty with international E. coli expert Barry Wanner, ...
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51 Escherichia coli phoA gene inactivation, insertion and ...
https://academicjournals.org/journal/AJMR/article-full-text-pdf/B3B9FF013240
procedure and the created E. coli mutants can be efficiently used for the study of ... We thank Barry Wanner (Purdue University, USA) for.
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52 a high-throughput method for measuring microbial colony ...
https://cir.nii.ac.jp/crid/1050845763888396672?lang=en
Colony-live --a high-throughput method for measuring microbial colony growth kinetics-- reveals diverse growth effects ... Wanner, Barry L.
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53 E. coli genotypes - - OpenWetWare
https://openwetware.org/wiki/E._coli_genotypes
A listed gene name means that gene carries a loss of function mutation, a Δ preceding a gene ... According to Barry Wanner, this is an unexpected result.
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54 Microbial musings – Summer 2022 - Microbiology Society
https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.001250?crawler=true&mimetype=application/pdf
This effort was aided following the landmark paper of Kirill Datsenko and Barry Wanner, where they described the use of.
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55 MODIFICATION OF THE ESCHERICHIA coli NISSLE 1917 ...
https://digitalcommons.uri.edu/cgi/viewcontent.cgi?article=1149&context=theses
method described by Datsenko and Wanner (18) was used to construct E. ... gene using an allelic replacement strategy developed by Dr. Barry Wanner and Dr.
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56 Technique quickly identifies bacteria for foo - EurekAlert!
https://www.eurekalert.org/pub_releases/2006-12/pu-tqi122806.php
The technique, called desorption electrospray ionization, ... Ongoing work by Talaty with international E. coli expert Barry Wanner, ...
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57 Murray_05.pdf - The University of Oklahoma
http://www.ou.edu/microarray/oumcf/Murray_05.pdf
phenol method (19) from E. coli cultures grown to mid-exponential or stationary ... served by others (Barry Wanner, personal communication).
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58 Grants Related To "LC ESI QOTOF METHOD DEVELOPMENT ...
https://www.paperdigest.org/related_grant/?grant_id=nih_7955880
In metabolomics, gas or liquid phase chromatographic separation ... NSF, DAVID SALT; BARRY WANNER; CLINTON CHAPPLE; DAVID RHODES;, PURDUE UNIVERSITY ...
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59 Sliding Clamp–DNA Interactions Are Required for Viability ...
https://hwi.buffalo.edu/wp-content/uploads/2016/12/sliding_clamp.pdf
lesions in the DNA, via a process termed translesion. DNA synthesis (TLS), display lower ... Barry Wanner (Purdue University) for plasmid.
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60 Regulation of Escherichia coli purF
https://www.jbc.org/article/S0021-9258(19)77685-6/pdf
genes of unknown function: orfl62 purF dedF. An open read- ... merville for suggestions and Rob Gunsalus and Barry Wanner for strains.
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61 Richard Wanner | Department of Sociology
https://soci.ucalgary.ca/manageprofile/profiles/richard-wanner
Eric Fong and Barry Edmonston John Deutchs Institute of Economic Policy, Queen''s University: McGill-Queen''''s University Press, 2011. pp. 133-152. Print.
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62 Control of conifer defoliators with neem-based systemic ...
https://www.cambridge.org/core/journals/canadian-entomologist/article/control-of-conifer-defoliators-with-neembased-systemic-bioinsecticides-using-a-novel-injection-device/C59D47D01BF9A6474DF0B29BF209F1BA
D. Barry Lyons ,. Kevin W. Wanner and. Taylor A. Scarr. Show author details ... A method for introducing water soluble chemicals into mature elms.
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63 New AAAS Fellows Recognized for Their Contributions to ...
https://www.aaas.org/news/new-aaas-fellows-recognized-their-contributions-advancing-science
Barry Philip Rosen, Florida International Univ. ... Bernstein Ratner, Univ. of Maryland, College Park; Eric Wanner, Russell Sage Foundation.
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64 Valsartan - Wikipedia
https://en.wikipedia.org/wiki/Valsartan
› wiki › Valsartan
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65 The no-SCAR (Scarless Cas9 Assisted Recombineering ...
https://core.ac.uk/download/pdf/78066455.pdf
Genome engineering methods in E. coli allow for easy to perform ... We thank Barry Wanner for providing plasmid pKD46 and Stanley.
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66 Index of Patents Issued from the United States Patent and ...
https://books.google.com/books?id=XN3dn2oUJjMC&pg=PA2171&lpg=PA2171&dq=barry+wanner+method&source=bl&ots=iWMIx4WR9P&sig=ACfU3U3FQisYuaI94AtuEOTKGfYWiFrNcw&hl=en&sa=X&ved=2ahUKEwjfwJCygMb7AhWmADQIHcq7Dh0Q6AF6BQjtARAD
Ward , Frederick Edmund , to Miles Laboratories , Inc. Process for Wanner ... Wolfgang ; Karl , Hubertus ; and Falchle , Jorg , Barry George ; and Hill ...
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67 Official Gazette of the United States Patent and Trademark ...
https://books.google.com/books?id=PXEbAQAAMAAJ&pg=RA2-PA84&lpg=RA2-PA84&dq=barry+wanner+method&source=bl&ots=7mu2O0om1M&sig=ACfU3U0zsr5kqm-4Q5FwpI2YO9JKlMU7yw&hl=en&sa=X&ved=2ahUKEwjfwJCygMb7AhWmADQIHcq7Dh0Q6AF6BQj2ARAD
Ward , David R. Method of training an operator in the use of detection Wakeley , Lillian ... Wanner , Barry L .; Lee , Ki - Sung ; and Metcalf , William W.
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68 Index of Patents Issued from the United States Patent Office
https://books.google.com/books?id=FYAS-IqYgPYC&pg=PA2171&lpg=PA2171&dq=barry+wanner+method&source=bl&ots=_dRYrQLp83&sig=ACfU3U3EnyQa18AHHCQ18AnNcCs79i_lkQ&hl=en&sa=X&ved=2ahUKEwjfwJCygMb7AhWmADQIHcq7Dh0Q6AF6BQj5ARAD
Method of manufacturing a pigment . 3,796,585 , 3-12-74 , Cl . 106-288.00b . Wanner , John C. , to Caterpillar Tractor Company . Guard assembly for track ...
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69 Light and Sound: A Text-book for Colleges and Technical Schools
https://books.google.com/books?id=EJk3AAAAMAAJ&pg=PA306&lpg=PA306&dq=barry+wanner+method&source=bl&ots=pgG3_R7JTW&sig=ACfU3U3TQetWfQNivke7rynv6IfID0z-8g&hl=en&sa=X&ved=2ahUKEwjfwJCygMb7AhWmADQIHcq7Dh0Q6AF6BQjqARAD
... for Colleges and Technical Schools William Suddards Franklin, Barry MacNutt ... basis of the practical form of pyrometer known as the Wanner pyrometer .
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70 Reports of Proceedings ... - Page 31 - Google Books Result
https://books.google.com/books?id=cRktAQAAMAAJ&pg=PA31-IA25&lpg=PA31-IA25&dq=barry+wanner+method&source=bl&ots=VllqmHJNIf&sig=ACfU3U3wvijpZeNxmTC9ojZDbzq6OR_HvQ&hl=en&sa=X&ved=2ahUKEwjfwJCygMb7AhWmADQIHcq7Dh0Q6AF6BQjpARAD
On wution of Mr. BARRY the Council voted to proceed to take a formal ballot ... be taken in the ame method as before , altbougu two members of the committee ...
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71 A Biographical Dictionary of Musicians - Page 38 - Google Books Result
https://books.google.com/books?id=bOk5AAAAIAAJ&pg=PA38&lpg=PA38&dq=barry+wanner+method&source=bl&ots=s24LN6TF4Y&sig=ACfU3U2OP0rNs182pF9ICauZS-KKqPd7vA&hl=en&sa=X&ved=2ahUKEwjfwJCygMb7AhWmADQIHcq7Dh0Q6AF6BQjrARAD
Wrote when he became leader in the Detmold court an admirable Method for Clarinet ... Bargheer , Adolf , brother of Karl L .; Spohr's pupil of Wanner and ...
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72 Printing Trade News - Volume 41 - Page 16 - Google Books Result
https://books.google.com/books?id=ng8hAQAAMAAJ&pg=RA6-PA16&lpg=RA6-PA16&dq=barry+wanner+method&source=bl&ots=3pWK-oRhUS&sig=ACfU3U1hfXRPwKdGB-nOlSgGz0DJYeWU9g&hl=en&sa=X&ved=2ahUKEwjfwJCygMb7AhWmADQIHcq7Dh0Q6AF6BQjsARAD
William H. Barry , a prominent young publisher of San Francisco , junior partner ... West and is modern in every respect and up - to - date in its methods .
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