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Google Keyword Rankings for : benzonase nuclease treatment
1
Benzonase® endonuclease - EMD Millipore
https://www.emdmillipore.com/Web-US-Site/en_CA/-/USD/ShowDocument-Pronet?id=201312.078
1 Benzonase® endonuclease is commonly used to degrade nucleic acids (RNA, DNA, etc.) to as small as. 3–5 base pairs (<6 kDa). Virus harvest treated with about.
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https://www.emdmillipore.com/Web-US-Site/en_CA/-/USD/ShowDocument-Pronet?id=201312.078
1 Benzonase® endonuclease is commonly used to degrade nucleic acids (RNA, DNA, etc.) to as small as. 3–5 base pairs (<6 kDa). Virus harvest treated with about.
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2
Benzonase® Nuclease
http://wolfson.huji.ac.il/purification/PDF/DNA_Removal/NOVAGEN_Benzonase.pdf
e) Benzonase treatment is not generally recommended for purification of proteins that must be nuclease free. However, depending on the processing methods, ...
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http://wolfson.huji.ac.il/purification/PDF/DNA_Removal/NOVAGEN_Benzonase.pdf
e) Benzonase treatment is not generally recommended for purification of proteins that must be nuclease free. However, depending on the processing methods, ...
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3
Design of Experiment Principles to Optimize Benzonase ...
https://cellculturedish.com/design-of-experiment-principles-to-optimize-benzonase-endonuclease-in-viral-gene-therapy-applications/
For over 30 years, Benzonase® endonuclease has been used for the removal of nucleic acids in virus vector and vaccine manufacturing as mandated ...
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https://cellculturedish.com/design-of-experiment-principles-to-optimize-benzonase-endonuclease-in-viral-gene-therapy-applications/
For over 30 years, Benzonase® endonuclease has been used for the removal of nucleic acids in virus vector and vaccine manufacturing as mandated ...
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4
Removing nucleic acids from nucleoid-associated proteins ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706162/
A Benzonase nuclease treatment, an ammonium sulfate precipitation and a heparin affinity chromatography were associated to remove nucleic acids bound to ...
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706162/
A Benzonase nuclease treatment, an ammonium sulfate precipitation and a heparin affinity chromatography were associated to remove nucleic acids bound to ...
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5
Benzonase wash for thawed frozen cells
https://www.med.unc.edu/masscytometry/wp-content/uploads/sites/1093/2020/04/Benzonase-Wash.docx
Benzonase Treatment for Frozen Cell Samples ... A nuclease treatment step is useful to avoid the cell clumping that results from dying cells releasing DNA.
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https://www.med.unc.edu/masscytometry/wp-content/uploads/sites/1093/2020/04/Benzonase-Wash.docx
Benzonase Treatment for Frozen Cell Samples ... A nuclease treatment step is useful to avoid the cell clumping that results from dying cells releasing DNA.
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6
Nucleic Acid Impurity Reduction in Viral Vaccine Manufacturing
https://bioprocessintl.com/upstream-processing/assays/nucleic-acid-impurity-reduction-in-viral-vaccine-manufacturing-349787/
Because process times for enzymatic reactions often measure in hours, Benzonase treatment is typically carried out in batch mode.
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https://bioprocessintl.com/upstream-processing/assays/nucleic-acid-impurity-reduction-in-viral-vaccine-manufacturing-349787/
Because process times for enzymatic reactions often measure in hours, Benzonase treatment is typically carried out in batch mode.
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7
Benzonase for Removal of Nucleic Acids in Protein Extraction
https://www.biocompare.com/Product-Reviews/352242-Removal-of-nucleic-acids-and-reduction-of-viscosity-during-protein-extraction/
Dilute the benzonase nuclease enzyme in 50% glycerol if required to reduce the concentration. Results Summary. After treating protein extracts ...
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https://www.biocompare.com/Product-Reviews/352242-Removal-of-nucleic-acids-and-reduction-of-viscosity-during-protein-extraction/
Dilute the benzonase nuclease enzyme in 50% glycerol if required to reduce the concentration. Results Summary. After treating protein extracts ...
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8
Production and purification of high-titer foamy virus vector for ...
https://www.sciencedirect.com/science/article/pii/S2329050116301826
Benzonase endonuclease is commonly used to reduce the amount of residual plasmid and cellular genomic DNA and RNA in the vector product. Treatment of FV ...
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https://www.sciencedirect.com/science/article/pii/S2329050116301826
Benzonase endonuclease is commonly used to reduce the amount of residual plasmid and cellular genomic DNA and RNA in the vector product. Treatment of FV ...
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9
Benzonase® Nuclease
http://www.bioon.com.cn/custom/merck/20090910/Benzonase.pdf
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ... e) Benzonase treatment is not generally recommended for purification of ...
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http://www.bioon.com.cn/custom/merck/20090910/Benzonase.pdf
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ... e) Benzonase treatment is not generally recommended for purification of ...
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10
Pierce™ Universal Nuclease for Cell Lysis
https://www.thermofisher.com/order/catalog/product/88700
Pierce Universal Nuclease for Cell Lysis helps to improve the separation of the lysate pellet from the supernatant, enhances filtration of the treated ...
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https://www.thermofisher.com/order/catalog/product/88700
Pierce Universal Nuclease for Cell Lysis helps to improve the separation of the lysate pellet from the supernatant, enhances filtration of the treated ...
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11
Benzonase® Nuclease/NucA Antibody MAB100634-100
https://www.rndsystems.com/products/benzonase-nuclease-nuca-antibody-1028917_mab100634
NucA (Benzonase) is also commonly used in bioprocessing applications to reduce viscosity of samples caused by genomic DNA. The optimum pH for enzyme activity is ...
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https://www.rndsystems.com/products/benzonase-nuclease-nuca-antibody-1028917_mab100634
NucA (Benzonase) is also commonly used in bioprocessing applications to reduce viscosity of samples caused by genomic DNA. The optimum pH for enzyme activity is ...
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12
GenFind V2 Supplemental Protocol: Isolation of gDNA from ...
https://media.beckman.com/-/media/pdf-assets/protocols/genomics-protocol-supplemental-genfind-v2-isolation-gdna-large-number-cells.pdf?la=en-us&hash=FBFF207FCECA8C54FA7EF571937859476DE4BF4B
Yield form different cell number treat with or without Benzonase nuclease. Without Benzonase nuclease treatment, 2 million cells yield about 20 µg DNA, when ...
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https://media.beckman.com/-/media/pdf-assets/protocols/genomics-protocol-supplemental-genfind-v2-isolation-gdna-large-number-cells.pdf?la=en-us&hash=FBFF207FCECA8C54FA7EF571937859476DE4BF4B
Yield form different cell number treat with or without Benzonase nuclease. Without Benzonase nuclease treatment, 2 million cells yield about 20 µg DNA, when ...
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13
Pre-digest of unprotected DNA by Benzonase improves the ...
https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-021-01067-0
Benzonase removes host DNA and unprotected microbial DNA because of its broad activity towards DNA substrates, which it cleaves into short ...
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https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-021-01067-0
Benzonase removes host DNA and unprotected microbial DNA because of its broad activity towards DNA substrates, which it cleaves into short ...
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14
Benzonase Endonuclease - MCLAB Products
https://www.mclab.com/Benzonase-Endonuclease.html
Benzonase Nuclease. BENU-100. 5 KU. >250u/ul. $56.10. Benzonase Nuclease. BENU -200 ... Pre-treatment of a protein sample improves its resolution on 2D gel ...
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https://www.mclab.com/Benzonase-Endonuclease.html
Benzonase Nuclease. BENU-100. 5 KU. >250u/ul. $56.10. Benzonase Nuclease. BENU -200 ... Pre-treatment of a protein sample improves its resolution on 2D gel ...
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15
Low-Cost, Fast, and Scalable Downstream Purification ...
https://www.bio-rad.com/webroot/web/pdf/psd/literature/Bulletin_7200.pdf
media screening, endonuclease treatment, and process scaling for the downstream ... Benzonase Nuclease was replaced with DENARASE. Endonuclease (Sartorius).
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https://www.bio-rad.com/webroot/web/pdf/psd/literature/Bulletin_7200.pdf
media screening, endonuclease treatment, and process scaling for the downstream ... Benzonase Nuclease was replaced with DENARASE. Endonuclease (Sartorius).
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16
Addition of nucleases directly to cell culture to facilitate ...
https://patents.google.com/patent/WO2018183426A1/en
For the 80 U/mL Benzonase® treated bioreactor conditions, the WHO recommended release spec of 10 ng/dose (N = 4 of 4) was achieved, while only 3 of the 4 post- ...
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https://patents.google.com/patent/WO2018183426A1/en
For the 80 U/mL Benzonase® treated bioreactor conditions, the WHO recommended release spec of 10 ng/dose (N = 4 of 4) was achieved, while only 3 of the 4 post- ...
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17
Benzonase® Nuclease (250U/µl) - ACE Biolabs.com
https://www.acebiolab.com/product_image/images/products/Figure/Enzyme/ER1017/Datasheet-ACE-ER1017%20Benzonase%20Nuclease.pdf?t=20210112103217
It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample improves its resolution on 2D gel ...
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https://www.acebiolab.com/product_image/images/products/Figure/Enzyme/ER1017/Datasheet-ACE-ER1017%20Benzonase%20Nuclease.pdf?t=20210112103217
It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample improves its resolution on 2D gel ...
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18
Benzonase Nuclease, >=250 unit | E1014-25KU | MILLIPORE
https://www.scientificlabs.co.uk/product/E1014-25KU
Benzonase nuclease, or endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity. Benzonase ...
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https://www.scientificlabs.co.uk/product/E1014-25KU
Benzonase nuclease, or endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity. Benzonase ...
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19
Evaluation of Plasmid DNA Removal from Lentiviral Vectors ...
https://www.researchgate.net/publication/7243725_Evaluation_of_Plasmid_DNA_Removal_from_Lentiviral_Vectors_by_Benzonase_Treatment
Optimal conditions of plasmid DNA removal by benzonase treatment were established by varying the temperature, time, and benzonase concentrations ...
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https://www.researchgate.net/publication/7243725_Evaluation_of_Plasmid_DNA_Removal_from_Lentiviral_Vectors_by_Benzonase_Treatment
Optimal conditions of plasmid DNA removal by benzonase treatment were established by varying the temperature, time, and benzonase concentrations ...
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20
https://www.teknova.com/catalog/product/view/id/26...
https://www.teknova.com/catalog/product/view/id/26213/s/benzonase-nuclease-25u-l/category/56/
› product › view › category
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https://www.teknova.com/catalog/product/view/id/26213/s/benzonase-nuclease-25u-l/category/56/
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21
(12) Patent Application Publication (10) Pub. No.
https://patentimages.storage.googleapis.com/0f/17/9a/dc828c5a643654/US20090017523A1.pdf
host cells with a virus, c) treating the cell culture with nuclease, and d) lysing the host ... nuclease used in step b) is BENZONASE(R).
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https://patentimages.storage.googleapis.com/0f/17/9a/dc828c5a643654/US20090017523A1.pdf
host cells with a virus, c) treating the cell culture with nuclease, and d) lysing the host ... nuclease used in step b) is BENZONASE(R).
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22
Current Gene Therapy - Semantic Scholar
https://pdfs.semanticscholar.org/3b89/a8e93f1b4ef159b3b0d6c44d0091f5e3cf0c.pdf
To overcome this challenge, a nuclease treatment was added to the process. Benzonase® is used to reduce the host cell. DNA content and its activity can be ...
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https://pdfs.semanticscholar.org/3b89/a8e93f1b4ef159b3b0d6c44d0091f5e3cf0c.pdf
To overcome this challenge, a nuclease treatment was added to the process. Benzonase® is used to reduce the host cell. DNA content and its activity can be ...
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23
Salt Active Nuclease E C H N IC A L O T E S
https://www.serva.de/www_root/documents/TN%20Salt%20Active%20Nuclease.pdf
High salt enables contaminating. DNA to dissociate from associated proteins and become available for degradation. But nucleases like. Benzonase®* are inhibited ...
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https://www.serva.de/www_root/documents/TN%20Salt%20Active%20Nuclease.pdf
High salt enables contaminating. DNA to dissociate from associated proteins and become available for degradation. But nucleases like. Benzonase®* are inhibited ...
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24
Benzonase (Recombinant, Molecular Biology Grade)
https://www.lubio.ch/assets/PDFs/RE1001_Benzonase_Datasheet.pdf
Benzonase treatment is not generally recommended for purification of proteins that must be nuclease free. However, depending on the processing methods, ...
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https://www.lubio.ch/assets/PDFs/RE1001_Benzonase_Datasheet.pdf
Benzonase treatment is not generally recommended for purification of proteins that must be nuclease free. However, depending on the processing methods, ...
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25
Sigma Chemical Company E1014-5KU
https://mms.mckesson.com/product/1052335/Sigma-Chemical-Company-E1014-5KU
Enzyme Benzonase Nuclease Recombinant ≥90% 5000 Units Sigma Chemical Company E1014-5KU.
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https://mms.mckesson.com/product/1052335/Sigma-Chemical-Company-E1014-5KU
Enzyme Benzonase Nuclease Recombinant ≥90% 5000 Units Sigma Chemical Company E1014-5KU.
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26
Considerations on the Use of Enzymes in the Downstream ...
https://www.openaccessjournals.com/articles/considerations-on-the-use-of-enzymes-in-the-downstream-processing-of-biopharmaceuticals.pdf
Benzonase® and Pulmozyme®) and RNases is ... treatment can be carried out at the end of the ... RNase is popular nuclease in the downstream.
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https://www.openaccessjournals.com/articles/considerations-on-the-use-of-enzymes-in-the-downstream-processing-of-biopharmaceuticals.pdf
Benzonase® and Pulmozyme®) and RNases is ... treatment can be carried out at the end of the ... RNase is popular nuclease in the downstream.
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27
Benzonase® Nuclease/NucA Products - Bio-Techne
https://www.bio-techne.com/t/benzonase-nuclease-nuca
Compare to Benzonase® Nuclease. Benzonase is a registered trademark of Merck KGaA Corp.Get 50% off R&D Systems® Recombinant S. marcescens NucA Protein!
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https://www.bio-techne.com/t/benzonase-nuclease-nuca
Compare to Benzonase® Nuclease. Benzonase is a registered trademark of Merck KGaA Corp.Get 50% off R&D Systems® Recombinant S. marcescens NucA Protein!
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28
Benzonase Nuclease - AtaGenix
http://en.atagenix.com/product_detail-41194.html
› product_detail-41194
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http://en.atagenix.com/product_detail-41194.html
› product_detail-41194
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29
Overcoming challenges of host cell DNA removal in vaccine ...
https://www.slideshare.net/pattnaik/nucleic-acid-removal-wvc-singapore-pattnaik
Nuclease Treatment; 11. ... Benzonase® digestion for live vaccines can reduce the infectivity of DNA by more than 100,000 fold ……. 11; 12.
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https://www.slideshare.net/pattnaik/nucleic-acid-removal-wvc-singapore-pattnaik
Nuclease Treatment; 11. ... Benzonase® digestion for live vaccines can reduce the infectivity of DNA by more than 100,000 fold ……. 11; 12.
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30
PIPPR® Total Mammalian Protein Extraction Kit Cat. No.
https://cobotechnologies.com/fileadmin/user_upload/Editor/C40101_PIPPR_-_100_reactions_-_FINAL_August-2019.pdf
storage, Benzonase® Nuclease should be stored at -20°C. Quality Control ... not intended for the diagnosis, prevention, or treatment of a disease.
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https://cobotechnologies.com/fileadmin/user_upload/Editor/C40101_PIPPR_-_100_reactions_-_FINAL_August-2019.pdf
storage, Benzonase® Nuclease should be stored at -20°C. Quality Control ... not intended for the diagnosis, prevention, or treatment of a disease.
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31
Using Pulmozyme DNase treatment in lentiviral vector ...
https://europepmc.org/article/pmc/4015072
DNase treatment. Enzymes used for plasmid DNA removal were Benzonase nuclease (Novagen, Darmstadt, Germany), and pharmaceutical-grade ...
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https://europepmc.org/article/pmc/4015072
DNase treatment. Enzymes used for plasmid DNA removal were Benzonase nuclease (Novagen, Darmstadt, Germany), and pharmaceutical-grade ...
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32
Modifying Enzymes - Aura Biotech
https://aurabiotech.com/modifying-enzymes/
Product description: Benzonase nuclease can effectively reduce the viscosity of protein samples, remove the contamination of nucleic acid in protein samples ...
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https://aurabiotech.com/modifying-enzymes/
Product description: Benzonase nuclease can effectively reduce the viscosity of protein samples, remove the contamination of nucleic acid in protein samples ...
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33
p13717 · nuca_serma - UniProt
https://www.uniprot.org/uniprot/P13717
Nuclease. Status. UniProtKB reviewed (Swiss-Prot). Organism. Serratia marcescens. Gene. nucA (nuc). Amino acids. 266. Protein existence.
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https://www.uniprot.org/uniprot/P13717
Nuclease. Status. UniProtKB reviewed (Swiss-Prot). Organism. Serratia marcescens. Gene. nucA (nuc). Amino acids. 266. Protein existence.
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34
Purification of baculovirus vectors using heparin affinity ...
https://www.cell.com/molecular-therapy-family/methods/pdf/S2329-0501(17)30033-5.pdf
Official journal of the American Society of Gene & Cell Therapy www.nature.com/mtm ... Supernatants were treated with Benzonase nuclease.
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https://www.cell.com/molecular-therapy-family/methods/pdf/S2329-0501(17)30033-5.pdf
Official journal of the American Society of Gene & Cell Therapy www.nature.com/mtm ... Supernatants were treated with Benzonase nuclease.
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35
SecNuc™ for AAV, lentiviral vectors and adenovirus
https://www.oxb.com/sites/default/files/OXB_SecNuc.pdf
recombinant nuclease, such as. Benzonase®, to treat harvested vector material prior to downstream purification. However, this treatment is.
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https://www.oxb.com/sites/default/files/OXB_SecNuc.pdf
recombinant nuclease, such as. Benzonase®, to treat harvested vector material prior to downstream purification. However, this treatment is.
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36
Improved, two-stage protein expression and purification via ...
https://www.biorxiv.org/content/10.1101/2020.01.09.900753v1.full
In commercial production after cell lysis, nucleases such as the non specific DNA/RNA endonuclease from S. marcescens (Benzonase ®) or ...
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https://www.biorxiv.org/content/10.1101/2020.01.09.900753v1.full
In commercial production after cell lysis, nucleases such as the non specific DNA/RNA endonuclease from S. marcescens (Benzonase ®) or ...
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37
Downstream bioprocessing of AAV vectors - BioInsights
https://insights.bio/immuno-oncology-insights/journal/article/352/downstream-bioprocessing-of-aav-vectors-industrial-challenges-regulatory-requirements
DNA reduction using nucleases ... Nuclease treatment has become a standard element in biomanufacturing since the commercialization of Benzonase. Although the cost ...
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https://insights.bio/immuno-oncology-insights/journal/article/352/downstream-bioprocessing-of-aav-vectors-industrial-challenges-regulatory-requirements
DNA reduction using nucleases ... Nuclease treatment has become a standard element in biomanufacturing since the commercialization of Benzonase. Although the cost ...
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38
Best practices in nucleic acid removal from viral vaccine ...
https://www.ijidonline.com/article/S1201-9712(14)01371-X/fulltext
Conclusion: Complete removal of host cell DNA is feasible by using combinations of nuclease treatment, tangential flow filtration and chromatography.
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https://www.ijidonline.com/article/S1201-9712(14)01371-X/fulltext
Conclusion: Complete removal of host cell DNA is feasible by using combinations of nuclease treatment, tangential flow filtration and chromatography.
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39
Benzonase Nuclease Ultrapure (Millipore) | Bioz
https://bioz.com/result/benzonase%20nuclease%20ultrapure/product/Millipore
Only nucleic acid treated with DNase retained its integrity whereas RNase and Benzonase treatment led to complete digestion.
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https://bioz.com/result/benzonase%20nuclease%20ultrapure/product/Millipore
Only nucleic acid treated with DNase retained its integrity whereas RNase and Benzonase treatment led to complete digestion.
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40
Benzonase® Nuclease | Krackeler Scientific, Inc.
https://www.krackeler.com/catalog/sigma/SIGMA/E1014
buffered aqueous glycerol solution
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https://www.krackeler.com/catalog/sigma/SIGMA/E1014
buffered aqueous glycerol solution
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41
Difference Between Benzonase and DNase
https://www.differencebetween.com/difference-between-benzonase-and-vs-dnase/
Summary – Benzonase vs DNase. Nuclease enzymes are widely used in different experimental procedures with regard to molecular biology and genetic ...
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https://www.differencebetween.com/difference-between-benzonase-and-vs-dnase/
Summary – Benzonase vs DNase. Nuclease enzymes are widely used in different experimental procedures with regard to molecular biology and genetic ...
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42
sc-202391.pdf - Benzonase Nuclease
https://datasheets.scbt.com/sc-202391.pdf
Treat symptomatically. Section 5 - FIRE FIGHTING MEASURES. Vapor Pressure (mmHG). Negligible. Upper Explosive Limit (%).
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https://datasheets.scbt.com/sc-202391.pdf
Treat symptomatically. Section 5 - FIRE FIGHTING MEASURES. Vapor Pressure (mmHG). Negligible. Upper Explosive Limit (%).
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43
Qproteome® Nuclear Protein Handbook - QIAGEN
https://www.qiagen.com/us/resources/download.aspx?id=0647953b-13b9-4811-88a0-54350b8fc2cc&lang=en
Benzonase® Nuclease and DTT Stock Solution should be stored at –20°C. Product Use Limitations. The Qproteome Nuclear Protein Kit ... treatment of a disease.
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https://www.qiagen.com/us/resources/download.aspx?id=0647953b-13b9-4811-88a0-54350b8fc2cc&lang=en
Benzonase® Nuclease and DTT Stock Solution should be stored at –20°C. Product Use Limitations. The Qproteome Nuclear Protein Kit ... treatment of a disease.
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44
Ultrasensitive isolation, identification and quantification of ...
https://academic.oup.com/nar/article/42/13/e108/1277581
Prior to treatment with Benzonase, samples were adjusted to 2 mM MgCl2 and then incubated with 12.5 U Benzonase nuclease (EMD Millipore) for ...
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https://academic.oup.com/nar/article/42/13/e108/1277581
Prior to treatment with Benzonase, samples were adjusted to 2 mM MgCl2 and then incubated with 12.5 U Benzonase nuclease (EMD Millipore) for ...
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45
Exploring a New Enzymatic Tool for AAV Production
https://www.genengnews.com/magazine/308/exploring-a-new-enzymatic-tool-for-aav-production/
To illustrate how alternatives to traditional nuclease can improve residual DNA clearance in high salt conditions, ArcticZymes presents a ...
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https://www.genengnews.com/magazine/308/exploring-a-new-enzymatic-tool-for-aav-production/
To illustrate how alternatives to traditional nuclease can improve residual DNA clearance in high salt conditions, ArcticZymes presents a ...
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46
Benzonase® Nuclease - VWR International
https://ie.vwr.com/store/product/10610196/benzonase-nuclease
Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein ...
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https://ie.vwr.com/store/product/10610196/benzonase-nuclease
Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein ...
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47
5-Carboxylcytosine is resistant towards phosphodiesterase I ...
https://pubs.rsc.org/en/content/articlepdf/2019/ra/c9ra04375f
treated with Benzonase protocol was consistent with the actual concentration. ... those treated with PDE1, Benzonase/PDE1 or P1 nuclease.
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https://pubs.rsc.org/en/content/articlepdf/2019/ra/c9ra04375f
treated with Benzonase protocol was consistent with the actual concentration. ... those treated with PDE1, Benzonase/PDE1 or P1 nuclease.
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48
Nuclease - Wikipedia
https://en.wikipedia.org/wiki/Nuclease
A nuclease is an enzyme capable of cleaving the phosphodiester bonds between nucleotides of nucleic acids. Nucleases variously effect single and double ...
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https://en.wikipedia.org/wiki/Nuclease
A nuclease is an enzyme capable of cleaving the phosphodiester bonds between nucleotides of nucleic acids. Nucleases variously effect single and double ...
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49
Development of a downstream process for the production of ...
https://www.nature.com/articles/s41598-020-72328-5
The nuclease treatment was performed in triplicates for both genotypes. The clarified virus was subjected to 250 U/mL Benzonase nuclease ...
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https://www.nature.com/articles/s41598-020-72328-5
The nuclease treatment was performed in triplicates for both genotypes. The clarified virus was subjected to 250 U/mL Benzonase nuclease ...
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50
Micrococcal Nuclease - NEB
https://www.neb.com/products/m0247-micrococcal-nuclease
› ... › Products
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https://www.neb.com/products/m0247-micrococcal-nuclease
› ... › Products
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51
Performance of pre-RT-qPCR treatments to discriminate
https://researchportal.helsinki.fi/files/122399966/Oristo_et_al_2018_Journal_of_Applied_Microbiology.pdf
applications of PMA/PMAxx, benzonase and RNase ' , Journal of Applied ... For PMA and nuclease treatment studies, we inactivated the viral aliquots in a dry.
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https://researchportal.helsinki.fi/files/122399966/Oristo_et_al_2018_Journal_of_Applied_Microbiology.pdf
applications of PMA/PMAxx, benzonase and RNase ' , Journal of Applied ... For PMA and nuclease treatment studies, we inactivated the viral aliquots in a dry.
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52
Microbial strains and processes for the manufacture of ...
https://www.google.com/patents/EP1068294A1
These strains secrete nuclease into the periplasm or growth medium in an amount ... 13 conducted with samples treated with benzonase (0.02 U), with culture ...
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https://www.google.com/patents/EP1068294A1
These strains secrete nuclease into the periplasm or growth medium in an amount ... 13 conducted with samples treated with benzonase (0.02 U), with culture ...
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53
An ameliorative protocol for the quantification of purine 5′,8 ...
https://www.frontiersin.org/articles/10.3389/fchem.2015.00047/full
... consisting of the nuclease benzonase and nuclease P1 enzymatic combination ... after treatment according to protocol D (Figure 6; ep1), the digestion ...
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https://www.frontiersin.org/articles/10.3389/fchem.2015.00047/full
... consisting of the nuclease benzonase and nuclease P1 enzymatic combination ... after treatment according to protocol D (Figure 6; ep1), the digestion ...
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54
CJD and Scrapie Require Agent-Associated Nucleic Acids for ...
https://medicine.yale.edu/lab/manuelidis/16%20nucleases_370011_5_v3.pdf
Benzonase (Bz), a powerful and protease free nuclease that completely fragments all nucleic acid types, ... ASB14 SOLUBILIZATION AND NUCLEASE TREATMENTS.
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https://medicine.yale.edu/lab/manuelidis/16%20nucleases_370011_5_v3.pdf
Benzonase (Bz), a powerful and protease free nuclease that completely fragments all nucleic acid types, ... ASB14 SOLUBILIZATION AND NUCLEASE TREATMENTS.
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55
SuperNuclease | SinoBiological
https://www.sinobiological.com/recombinant-proteins/supernuclease-ssnp01
SuperNuclease is a recombined Serratia marcescens' extracellular endonuclease. The sequence is same with Benzonase® (Benzonase is a trademark of Merck KGaA, ...
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https://www.sinobiological.com/recombinant-proteins/supernuclease-ssnp01
SuperNuclease is a recombined Serratia marcescens' extracellular endonuclease. The sequence is same with Benzonase® (Benzonase is a trademark of Merck KGaA, ...
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56
Antibody to DNA detects scrapie but not normal prion protein
https://www.pnas.org/doi/10.1073/pnas.0307825100
Alternatively, we treated the BHs with nuclease (benzonase) followed by immunocapture with OCD4. No PrP was immunocaptured by OCD4 in the presence of the added ...
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https://www.pnas.org/doi/10.1073/pnas.0307825100
Alternatively, we treated the BHs with nuclease (benzonase) followed by immunocapture with OCD4. No PrP was immunocaptured by OCD4 in the presence of the added ...
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57
How to prevent "sticky" cells clogging machine - CyTOForum
http://cytoforum.stanford.edu/viewtopic.php?f=4&t=943&start=10
Hi all, As our standard treatment, the HIMC adds Benzonase to our cell media (RPMI + pen/strep/glutamine + heat-inactivated FBS) during ...
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http://cytoforum.stanford.edu/viewtopic.php?f=4&t=943&start=10
Hi all, As our standard treatment, the HIMC adds Benzonase to our cell media (RPMI + pen/strep/glutamine + heat-inactivated FBS) during ...
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58
Robust co-immunoprecipitation with mass spectrometry for ...
https://www.future-science.com/doi/pdf/10.2144/btn-2021-0074
To compare the impact of Benzonase and. DNase treatment on chromatin regulator interactions, protein–protein interactions of the germline- ...
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https://www.future-science.com/doi/pdf/10.2144/btn-2021-0074
To compare the impact of Benzonase and. DNase treatment on chromatin regulator interactions, protein–protein interactions of the germline- ...
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59
RAP-MS: A Method to Identify Proteins that Interact Directly ...
https://guttmanlab.caltech.edu/files/2021/08/McHugh-Guttman2018_Protocol_RAP-MSAMethodToIdentifyProtein.pdf
Benzonase nuclease. 15. Detergent removal columns (e.g., Life Technologies HiPPR). Fig. 4 Example of SILAC ratio plot for proteins from RAP-MS captures.
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https://guttmanlab.caltech.edu/files/2021/08/McHugh-Guttman2018_Protocol_RAP-MSAMethodToIdentifyProtein.pdf
Benzonase nuclease. 15. Detergent removal columns (e.g., Life Technologies HiPPR). Fig. 4 Example of SILAC ratio plot for proteins from RAP-MS captures.
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60
Linking nuclear matrix-localized PIAS1 to chromatin ...
https://www.jbc.org/article/S0021-9258(21)01002-4/pdf
nuclease digestion that all PIAS family proteins maintain nu- ... DNA upon 4-h Benzonase treatment, indicating that bulk.
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https://www.jbc.org/article/S0021-9258(21)01002-4/pdf
nuclease digestion that all PIAS family proteins maintain nu- ... DNA upon 4-h Benzonase treatment, indicating that bulk.
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61
RNA molecules stimulate prion protein conversion
https://geiselmed.dartmouth.edu/supattapone/wp-content/uploads/sites/80/2021/02/Nature_2003.pdf
In vitro PrPres amplification was also abolished by treatment with purified pan- creatic RNase A, RNase T1, micrococcal nuclease, or benzonase. (Fig. 1a).
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https://geiselmed.dartmouth.edu/supattapone/wp-content/uploads/sites/80/2021/02/Nature_2003.pdf
In vitro PrPres amplification was also abolished by treatment with purified pan- creatic RNase A, RNase T1, micrococcal nuclease, or benzonase. (Fig. 1a).
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62
Nuclease at Thomas Scientific
https://www.thomassci.com/scientific-supplies/Nuclease
Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens . It degrades all forms of DNA and RNA (single stranded, ...
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https://www.thomassci.com/scientific-supplies/Nuclease
Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens . It degrades all forms of DNA and RNA (single stranded, ...
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63
Safety Data Sheet - Takara Bio
https://www.takarabio.com/documents/SDS/S3569/S3569-EGHS-EN.pdf
Treat symptomatically. 5. Fire-fighting measures. Suitable Extinguishing Media. Use extinguishing measures that are appropriate to local ...
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https://www.takarabio.com/documents/SDS/S3569/S3569-EGHS-EN.pdf
Treat symptomatically. 5. Fire-fighting measures. Suitable Extinguishing Media. Use extinguishing measures that are appropriate to local ...
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64
New Nuclease from Extremely Psychrophilic Microorganism ...
https://link.springer.com/article/10.1007/s12033-018-0142-z
Nucleases are an important group of hydrolases that degrade nucleic ... obtained from mesophilic bacterium Serratia marcescens (Benzonase®; ...
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https://link.springer.com/article/10.1007/s12033-018-0142-z
Nucleases are an important group of hydrolases that degrade nucleic ... obtained from mesophilic bacterium Serratia marcescens (Benzonase®; ...
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65
Phosphorylation at Serines 216 and 221 Is Important ... - PLOS
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075381
For DNase/RNase treatment, 50 ul of nuclear extract was separately ... For the benzonase nuclease experiment, nuclei were treated with 500 ...
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https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075381
For DNase/RNase treatment, 50 ul of nuclear extract was separately ... For the benzonase nuclease experiment, nuclei were treated with 500 ...
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66
UltraNuclease GMP-grade-Yeasen
https://www.yeasenbiotech.com/productdetail/2405
UltraNuclease GMP-grade from Yeasen is expressed and purified in Escherichia coli (E. coli) by genetically engineered, and prepared under the GMP ...
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https://www.yeasenbiotech.com/productdetail/2405
UltraNuclease GMP-grade from Yeasen is expressed and purified in Escherichia coli (E. coli) by genetically engineered, and prepared under the GMP ...
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67
Protocol for Reducing Cell Clumping in Single Cell Suspensions
https://www.stemcell.com/how-to-reduce-cell-clumping-in-single-cell-suspensions-with-dnase.html
› ... › Centrifugation
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https://www.stemcell.com/how-to-reduce-cell-clumping-in-single-cell-suspensions-with-dnase.html
› ... › Centrifugation
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68
guideline-quality-non-clinical-clinical-aspects-gene-therapy ...
https://www.ema.europa.eu/en/documents/scientific-guideline/guideline-quality-non-clinical-clinical-aspects-gene-therapy-medicinal-products_en.pdf
Gene therapy medicinal products, advanced therapy medicinal ... approaches such as CRISPR-Cas, Zinc finger nucleases (ZFNs) or TALENs.
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https://www.ema.europa.eu/en/documents/scientific-guideline/guideline-quality-non-clinical-clinical-aspects-gene-therapy-medicinal-products_en.pdf
Gene therapy medicinal products, advanced therapy medicinal ... approaches such as CRISPR-Cas, Zinc finger nucleases (ZFNs) or TALENs.
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69
Qproteome® Bacterial Protein Preparation Handbook - Labettor
https://labettor.com/uploads/products/protocols/1115.pdf
Benzonase® Nuclease. 3 x 80 цl (25 U/ml). Lysozyme ... treatment of a disease. ... Lysis Buffer that is supplemented with lysozyme and Benzonase® Nuclease,.
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https://labettor.com/uploads/products/protocols/1115.pdf
Benzonase® Nuclease. 3 x 80 цl (25 U/ml). Lysozyme ... treatment of a disease. ... Lysis Buffer that is supplemented with lysozyme and Benzonase® Nuclease,.
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70
PBMC Isolation, cryopreservation and thawing
https://www.helmholtz-muenchen.de/fileadmin/Immunmonitoring/pdf/PBMC_Isolation_Cryo_Thaw.pdf
Benzonase Nuclease (purity >99%, Novagen Merck Biosciences # 71206-3) ... incorporated a Benzonase treatment step in the thawing procedure ...
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https://www.helmholtz-muenchen.de/fileadmin/Immunmonitoring/pdf/PBMC_Isolation_Cryo_Thaw.pdf
Benzonase Nuclease (purity >99%, Novagen Merck Biosciences # 71206-3) ... incorporated a Benzonase treatment step in the thawing procedure ...
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71
Benzonase® Nuclease - The Wolfson Centre for Applied ...
https://studylib.net/doc/8439041/benzonase%C2%AE-nuclease---the-wolfson-centre-for-applied-stru...
® Benzonase Nuclease Benzonase Nuclease Benzonase Nuclease, ... Samples were treated with various concentrations of Benzonase Nuclease at 0°C, ...
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https://studylib.net/doc/8439041/benzonase%C2%AE-nuclease---the-wolfson-centre-for-applied-stru...
® Benzonase Nuclease Benzonase Nuclease Benzonase Nuclease, ... Samples were treated with various concentrations of Benzonase Nuclease at 0°C, ...
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72
Extracellularly secreted nuclease - patentCut
https://patentcut.com/9217139
The nucleases disclosed in Patent Documents 1 and 2, and Benzonase ... such as thermal treatment; a method of using a difference in molecular weight, ...
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https://patentcut.com/9217139
The nucleases disclosed in Patent Documents 1 and 2, and Benzonase ... such as thermal treatment; a method of using a difference in molecular weight, ...
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73
Optimization Of Benzonase Endonuclease Use In Virus ...
https://www.bioprocessonline.com/doc/optimization-of-benzonase-endonuclease-use-in-virus-purification-0001
The use of Benzonase® endonuclease additionally increases the yield in virus purification, protects the downstream chromatography and filter ...
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https://www.bioprocessonline.com/doc/optimization-of-benzonase-endonuclease-use-in-virus-purification-0001
The use of Benzonase® endonuclease additionally increases the yield in virus purification, protects the downstream chromatography and filter ...
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74
Preparing Whole Cell Protein Extracts – Basic Protocol
https://www.uthsc.edu/research/institutional-cores/pmc/documents/03-preparing-whole-cell-protein-extracts-for-differential-expession-analysis.docx
... wild type vs mutant, treated vs untreated, individual time points, etc. ... Alternatively, use Pierce Universal Nuclease for Cell Lysis (P/N 88700) to.
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https://www.uthsc.edu/research/institutional-cores/pmc/documents/03-preparing-whole-cell-protein-extracts-for-differential-expession-analysis.docx
... wild type vs mutant, treated vs untreated, individual time points, etc. ... Alternatively, use Pierce Universal Nuclease for Cell Lysis (P/N 88700) to.
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75
MilliporeSigma™ Novagen™ Benzonase™ Nuclease GSA_VA
https://www.fishersci.com/shop/products/novagen-benzonase-nuclease/707463
Removal of nucleic acids from recombinant proteins Shop MilliporeSigma™ Novagen™ Benzonase™ Nuclease at Fishersci.com.
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https://www.fishersci.com/shop/products/novagen-benzonase-nuclease/707463
Removal of nucleic acids from recombinant proteins Shop MilliporeSigma™ Novagen™ Benzonase™ Nuclease at Fishersci.com.
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76
How do you inactivate Benzonase? – Ulmerstudios
https://ulmerstudios.com/blog/how-do-you-inactivate-benzonase/
At 6 M urea, enzyme activity first increases, then decreases over time. At 7 M urea, Benzonase Nuclease denatures after 15 minutes, and activity ...
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https://ulmerstudios.com/blog/how-do-you-inactivate-benzonase/
At 6 M urea, enzyme activity first increases, then decreases over time. At 7 M urea, Benzonase Nuclease denatures after 15 minutes, and activity ...
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77
Benzonase Nuclease (Merck) - [PDF Document]
https://fdocuments.us/document/benzonase-nuclease-merck.html
By completely digesting nucleic acids, Benzonase® Nuclease reduces the viscosity of your samples – yet is perfectly safe for proteins. So you ...
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https://fdocuments.us/document/benzonase-nuclease-merck.html
By completely digesting nucleic acids, Benzonase® Nuclease reduces the viscosity of your samples – yet is perfectly safe for proteins. So you ...
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78
Salt Active Nuclease High Quality (Bioprocessing grade)
https://arcticzymes.com/products/enzymes/salt-active-nuclease-high-quality-bioprocessing-grade/
Properties · Temperature: 5 – 40°C, optimal: ~35°C · Salt concentrations (NaCl / KCl): 150 mM – 900 mM, optimal: 400 - 650 mM · Mg2+: >1 mM is required for ...
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https://arcticzymes.com/products/enzymes/salt-active-nuclease-high-quality-bioprocessing-grade/
Properties · Temperature: 5 – 40°C, optimal: ~35°C · Salt concentrations (NaCl / KCl): 150 mM – 900 mM, optimal: 400 - 650 mM · Mg2+: >1 mM is required for ...
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79
Advances in DNA Research and Application: 2013 Edition
https://books.google.com/books?id=v2W1EUFfxyQC&pg=PA223&lpg=PA223&dq=benzonase+nuclease+treatment&source=bl&ots=TBS4e5Gl4X&sig=ACfU3U3gMjo_bhiZKE5PbTuebqDN430_mA&hl=en&sa=X&ved=2ahUKEwizxMKFsMb7AhUCjIkEHUIJAikQ6AF6BQjCAhAD
The cells are lysed, and then the cells are treated with a nuclease, such as benzonase. Cell debris is removed by precipitation, such as by salt (e.g., ...
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https://books.google.com/books?id=v2W1EUFfxyQC&pg=PA223&lpg=PA223&dq=benzonase+nuclease+treatment&source=bl&ots=TBS4e5Gl4X&sig=ACfU3U3gMjo_bhiZKE5PbTuebqDN430_mA&hl=en&sa=X&ved=2ahUKEwizxMKFsMb7AhUCjIkEHUIJAikQ6AF6BQjCAhAD
The cells are lysed, and then the cells are treated with a nuclease, such as benzonase. Cell debris is removed by precipitation, such as by salt (e.g., ...
→ Check Latest Keyword Rankings ←
80
Experimental Approaches For The Investigation Of Innate ...
https://books.google.com/books?id=UHvQDAAAQBAJ&pg=PA59&lpg=PA59&dq=benzonase+nuclease+treatment&source=bl&ots=32YpXAKONe&sig=ACfU3U2UJcSikeTUBGkYIwF4JTtwaK5HvQ&hl=en&sa=X&ved=2ahUKEwizxMKFsMb7AhUCjIkEHUIJAikQ6AF6BQi1AhAD
... 10XPBS Amerixan AB11072-04000 Bioanalytical Benzonase Treatment Tris-HCI ... Baker 2444-01 Benzonase Sigma-Aldrich E1014 Endonuclease used to Nuclease ...
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https://books.google.com/books?id=UHvQDAAAQBAJ&pg=PA59&lpg=PA59&dq=benzonase+nuclease+treatment&source=bl&ots=32YpXAKONe&sig=ACfU3U2UJcSikeTUBGkYIwF4JTtwaK5HvQ&hl=en&sa=X&ved=2ahUKEwizxMKFsMb7AhUCjIkEHUIJAikQ6AF6BQi1AhAD
... 10XPBS Amerixan AB11072-04000 Bioanalytical Benzonase Treatment Tris-HCI ... Baker 2444-01 Benzonase Sigma-Aldrich E1014 Endonuclease used to Nuclease ...
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81
Benzonase Nuclease (Merck) - [PDF Document] - Cupdf
https://cupdf.com/document/benzonase-nuclease-merck.html
In particular, Benzonase Nuclease completely digests nucleic acids (all kinds of DNA and RNA) to 5-monophosphate-terminated oligonucleotides 3 ...
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https://cupdf.com/document/benzonase-nuclease-merck.html
In particular, Benzonase Nuclease completely digests nucleic acids (all kinds of DNA and RNA) to 5-monophosphate-terminated oligonucleotides 3 ...
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82
Benzonase Nuclease Antibodies - Novus Biologicals
https://www.novusbio.com/primary-antibodies/benzonase-nuclease
Benzonase Nuclease Antibodies available through Novus Biologicals. Browse our Benzonase Nuclease Antibodies all backed by our Guarantee+.
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https://www.novusbio.com/primary-antibodies/benzonase-nuclease
Benzonase Nuclease Antibodies available through Novus Biologicals. Browse our Benzonase Nuclease Antibodies all backed by our Guarantee+.
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