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1 Combining Optical Reporter Proteins with Different Half-lives ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4688563/
The EGFP degradation half-life was approximately 15 hours without CHX treatment and approximately 83 minutes in CHX-treated cells. In both ...
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2 tdTomato :: Fluorescent Protein Database
https://www.fpbase.org/protein/tdtomato/
tdTomato is a basic (constitutively fluorescent) orange fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a somewhat ...
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3 Selection Guide: Fluorescent Proteins
https://www.takara.co.kr/file/manual/D121216.html
tdTomato detected in a SCID mouse ... fluorescent proteins are particularly useful for determining protein half-life and protein transport pathways, ...
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4 In vivo study of gene expression with an enhanced ... - bioRxiv
https://www.biorxiv.org/content/10.1101/564690v1.full.pdf
lost due to the long half-life of the fluorescent proteins. To better detect dynamics, fluorescent transcriptional reporters can be ...
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5 Stem Cell Pluripotency Genes Klf4 and Oct4 Regulate ...
https://www.ahajournals.org/doi/pdf/10.1161/CIRCULATIONAHA.120.046672?download=true
that the half-life of tdTomato is 3 and a half days,28 we reasoned that these cells represented SMC currently in.
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6 Vivo with a loxP-Flanked tdTomato Reporter M - CORE
https://core.ac.uk/download/pdf/154892772.pdf
tdTomato protein in transfected cells of genetically modified ... half-life of these fluorescent proteins (Figure 2A).27 We found no.
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7 tdTomato fluorescent protein - Takara Bio
https://www.takarabio.com/products/gene-function/fluorescent-proteins/fluorescent-protein-plasmids/red-fluorescent-proteins/tdtomato-fluorescent-protein
tdTomato is an exceptionally bright red fluorescent protein—6X brighter than EGFP. tdTomato's emission wavelength (581 nm) and brightness make it ideal for ...
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8 EpoR-tdTomato-Cre mice enable identification of EpoR ...
https://ashpublications.org/blood/article/138/20/1986/476101/EpoR-tdTomato-Cre-mice-enable-identification-of
EpoR-tdTomato-Cre mouse line provides a powerful tool for iden. ... a long half-life,52 we reasoned that a long half-life of tdTomato could ...
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9 In vivo study of gene expression with an enhanced dual ... - eLife
https://elifesciences.org/articles/46181
Compared to FPs with a half-life of 20 hr, FPs with a half-life of 2 hr have a 90% shorter response time (time to achieve 50% maximal intensity) ...
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10 Rapid, Single-Cell Analysis and Discovery of Vectored mRNA ...
https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(17)30289-5
CreERT2-tdTomato mice were treated as in (A). (D) The half-life (t1/2) of the Tom+p16high cells was determined in the indicated organs from ...
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11 A Transgenic Mouse Line Expressing the Red Fluorescent ...
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0129934
TdTomato expression was observed in all expected brain regions; however, ... whereas only about half of GAD65 exists as active holoenzyme.
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12 Regulation of gene expression in gene therapy - Slajd 1
http://biotka.mol.uj.edu.pl/zbm/handouts/2015/JD/3_WYKLAD_10_19_2015.pdf
stability - protein is very stable (half-life is 50 hours) ... mOrange2 and tdTomato are members of the mFruit family pioneered by Tsien and.
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13 d2ECFP Sequence and Map - SnapGene
https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=d2ECFP
ECFP destabilized by residues 422-461 of mouse ornithine decarboxylase, giving an in vivo half-life of ~2 hours.
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14 Reporter gene comparison demonstrates interference of ...
https://www.nature.com/articles/s41598-020-80451-6
tdTomato is an extremely bright red fluorescent protein, being one of ... to facilitate lysosomal breakdown and therefore shorten half-life.
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15 a modelling approach to tandem fluorescent timer design and ...
https://www.huber.embl.de/pub/pdf/Barry2015.pdf
differences in protein half-life (http://chronos.embl.de/TimerQuant). ... despite tdTomato having a shorter reported maturation time than. TagRFP.
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16 Cells exhibiting strong p16INK4a promoter activation ... - PNAS
https://www.pnas.org/doi/10.1073/pnas.1818313116
The half-life of the knocked-in transcript was shorter than that of the ... fragments of p16INK4a and tdTomato were cloned, and a standard ...
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17 Researchers create p16-Cre ERT2 -tdTomato mouse model to ...
https://www.news-medical.net/news/20201007/Researchers-create-p16-Cre-ERT2-tdTomato-mouse-model-to-characterize-in-vivo-senescent-cells.aspx
They found tdTomato-positive p16 high cells detectable in all organs, which were enriched with age. They also found that these cells failed to ...
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18 Rainbow Vectors for Broad-Range Bacterial Fluorescence ...
https://pdfs.semanticscholar.org/8c71/21921dbe2d9de777b2f1fbf620fffdc79c89.pdf
GFPmut3, eYFP, tdTomato, dsRedExpress, mCherry, mKeima and mPlum had a high sensitiv- ity to photobleaching with short half-life and a fast ...
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19 B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J
https://www.jax.org/strain/007909
Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination. This strain is congenic on the C57BL/6J genetic ...
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20 Systematic In Vivo Characterization of Fluorescent Protein ...
https://pubs.acs.org/doi/pdf/10.1021/acssynbio.1c00387
dark reversion (with a half-life of ∼30 min30), which allows the ... (mCherry and tdTomato) and TagRFP (mKate2) are known.
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21 Simultaneous Acquisition of Multicolor Information From ...
https://www.frontiersin.org/articles/10.3389/fnins.2018.00885/full
With GFP/tdTomato double-labeled samples in resin, we obtained the ... and localization information indicated that more than half of the ...
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22 SUPPLEMENTARY APPENDIX - Haematologica
https://haematologica.org/article/download/7828/50819
REC mice (EpoGFP/GFP:Tg3.3K-EpoE3:Rosa26LSL-tdTomato:TgEpoCre genotype)1 ... determine the plasma half-lives of ESAs because EPO-EPOR complexes are degraded.
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23 The world's first successful identification a - EurekAlert!
https://www.eurekalert.org/news-releases/684913
They found tdTomato-positive p16 high cells detectable in all organs, which were enriched with age. They also found that these cells failed to ...
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24 The world's first successful identification and ... - ScienceDaily
https://www.sciencedaily.com/releases/2020/10/201007093627.htm
They found tdTomato-positive p16 high cells detectable in all organs, which were enriched with age. They also found that these cells failed to ...
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25 Luciferase/tdTomato Dual-Reporter B16/F10 Cell Line
https://www.alstembio.com/web/documents/Datasheet_Luciferase_reporter_cell_line-B16F10-LRL05.pdf
Luciferase/tdTomato dual-reporter B16/F10 (B16/F10-Luc-RFP) cell line is ... The Steady-Glo® Reagent provides stable luminescence with a half-life.
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26 Strategies to investigate protein turnover with fluorescent ...
https://www.aimspress.com/article/doi/10.3934/biophy.2020008?viewType=HTML
tdTomato, 554, 581, 0.69, 138000, 95, dimer, 60, 4.7, [43],[80], [15],[52] ... Measurement of protein half-lives with an FP fused to the protein of interest ...
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27 Adenovirus tdTomato (200 µl) - LifeSct LLC
http://www.lifesct.com/Adenovirus-tdTomato
Because the two subunits of tdTomato are linked covalently, it behaves as a monomer, and has been used successfully for N- and C- terminal fusions. It shows ...
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28 The world's first successful identification and characterization ...
https://www.ims.u-tokyo.ac.jp/imsut/en/about/press/page_00021.html
They found tdTomato-positive p16 high cells detectable in all organs, which were enriched with age. They also found that these cells failed to ...
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29 Vivo with a loxP-Flanked tdTomato Reporter M - Harvard DASH
https://dash.harvard.edu/bitstream/handle/1/34651779/5734870.pdf?sequence=1
tdTomato protein in transfected cells of genetically modified ... half-life of these fluorescent proteins (Figure 2A).27 We found no.
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30 Tdtomato (The Jackson Laboratory) | Bioz | Ratings For Life ...
https://www.bioz.com/result/tdtomato/product/The%20Jackson%20Laboratory
Tdtomato, supplied by The Jackson Laboratory, used in various techniques. ... points showed that the half-life of GALC protein, in vitro , is about 3 hours.
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31 Simple Summary - MDPI
https://mdpi-res.com/d_attachment/cancers/cancers-14-02508/article_deploy/cancers-14-02508.xml
( G ) The half-life of TdTomato, GFP, and dynGFP were compared by measuring relative MFI on flow cytometry. B16F10 cells were pre-treated with TGF-β (48 h ...
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32 knocks over can* "Pick that up NOW" (Entropy Zero 2) - YouTube
https://www.youtube.com/watch?v=pucNQQ6nfVc
Tomato's Trash
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33 Dynamic Visualization of TGF-β/SMAD3 Transcriptional ...
https://www.mdpi.com/2072-6694/14/10/2508/htm
(G) The half-life of TdTomato, GFP, and dynGFP were compared by measuring relative MFI on flow cytometry. B16F10 cells were pre-treated with TGF-β (48 h for ...
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34 Comparative assessment of fluorescent proteins for in vivo ...
https://www.molbiolcell.org/doi/10.1091/mbc.e16-01-0063
GFP and mNG displayed similar photobleaching half-lives, with mNG being ... To determine the half-life of a given fluorescent protein, ...
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35 red fluorescent protein for fluorescence imaging in vivo
https://shulab.ucsf.edu/Pub_Yu%20ProSci2016.pdf
and tdTomato-labeled plasma membrane, time-lapse imaging to visualize the dynamics ... ments demonstrated that the half-life of iBlueberry.
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36 A Subpopulation of Microglia Generated in the Adult Mouse ...
https://www.jneurosci.org/content/jneuro/41/38/7942.full.pdf
We observed a population of new TdTomato-expressing microglia at 6 months ... can divide to slowly generate new microglia throughout life.
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37 Heterogeneity in Cell Age Contributes to Functional Diversity ...
https://journals.aai.org/jimmunol/article/206/3/465/107988/Cutting-Edge-Heterogeneity-in-Cell-Age-Contributes
Modeling NK cell homeostatic turnover in iNkp46tdTomato mice reveals ... Fitting a one-phase decay model estimated the half-life of peripheral blood NK ...
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38 The aryl hydrocarbon receptor contributes to tissue adaptation ...
https://rupress.org/jem/article/219/4/e20210970/213047/The-aryl-hydrocarbon-receptor-contributes-to
Their lifetime in tissues is variable, with a half-life of <1 d in blood ... AHR-TdTomato expression in eosinophils was lowest in the bone ...
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39 Accurate, strong, and stable reporting of choroid plexus ...
https://escholarship.org/content/qt02d7t09s/qt02d7t09s_noSplash_c89f9150882e2ff01fd53981c0a781c8.pdf
Two of the tdTomato lines were bred to homozygosity, and homozygous mice ... Fewer than half ... was not tightly linked to TTR expression over the life-.
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40 Kauffman et al.pdf - The Jaklenec Group
https://jaklenecgroup.mit.edu/sites/default/files/images/Kauffman%20et%20al.pdf
tdTomato protein in transfected cells of genetically modified ... half-life of these fluorescent proteins (Figure 2A).27 We found no.
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41 Esrrb extinction triggers dismantling of naïve pluripotency and ...
https://www.embopress.org/doi/10.15252/embj.201695476
Examination of targeted Esrrb-tdTomato (E-tdT) cells showed that the Esrrb-tdTomato fusion protein had a similar half-life to that of endogenous Esrrb (Appendix ...
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42 Heterogeneity in Readouts of Canonical Wnt Pathway Activity ...
https://repository.upenn.edu/cgi/viewcontent.cgi?article=1644&context=statistics_papers
Axin2-CreERT2-tdTomato-marked cells using single cell gene expression profiling and ... of Tamoxifen in the bloodstream given its 12–18 hour half-life.
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43 Fluorescent proteins: a cell biologist's user guide
https://www.einsteinmed.edu/labs/erik-snapp/publications/snapp2009ticbfpuserguide.pdf
lives) to enable prolonged imaging of cells. Opinion ... Tandem dimer FPs such as tdKatushka2 and tdTomato ... half-lives of 2 h have been achieved [23].
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44 Cells exhibiting strong p16INK4a promoter activation in vivo ...
https://digitalcommons.wustl.edu/cgi/viewcontent.cgi?article=8483&context=open_access_pubs
the mRNA half-life of the endogenous p16INK4a and Arf ... abundance, fragments of p16INK4a and tdTomato were cloned, and a standard.
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45 Validated Antibodies - iDISCO method
https://idisco.info/validated-antibodies/
Phospho-S6 (Ser244/247)- Life Technologies 44923G ... RFP (tdtomato/mCherry)- Rockland 600-401-379 ... Note: this antibody has a short half-life at 4°c.
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46 MMRRC:043539-UNC
https://www.mmrrc.org/catalog/sds.php?mmrrc_id=43539
Animals · Cell Proliferation · Cellular Senescence/genetics · Cyclin-Dependent Kinase Inhibitor p16/genetics · Enzyme Activation · Fibroblasts/metabolism · Half-Life ...
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47 Identification of Stem Cells in the Epithelium of the Stomach ...
https://www.gastrojournal.org/article/s0016-5085(16)35113-7/fulltext
The half-life of parietal cells and chief cells is several months (see diagram ... (E, F) The tdTomato expression in corpus epithelium from ...
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48 Monitoring C3aR Expression Using a Floxed tdTomato-C3aR ...
https://www.jimmunol.org/content/199/2/688.full.pdf
penicillin, and streptomycin were all from Life Technologies. Animals ... The first fragment was a 3.1-kb fragment encoding the 39 half of.
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49 Long Live the Microglia! Studies Trace Their Lifespans in Mice ...
https://www.alzforum.org/news/research-news/long-live-microglia-studies-trace-their-lifespans-mice-and-humans
Life and death were less balanced when CD11b-tdTomato/Iba1-GFP double transgenic mice were crossed to an APP/PS1 background.
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50 Confocal Microscopy - The Fluorescent Protein Color Palette
https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/confocal/applications/fpcolorpalette/
CyPet is about half as bright as EGFP and two-thirds as bright as Cerulean, ... and tdTomato (a tandem dimer), all of which have fluorescence emission ...
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51 jGCaMP8 Calcium indicators - Janelia Research Campus
https://www.janelia.org/jgcamp8-calcium-indicators
... such as P{10XUAS-IVS-myr::tdTomato} to aid in identifying the neurons (see ... Max dF/F, Half-rise time (ms), Time to peak (ms), Half-decay time (ms) ...
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52 Redundancy and multifunctionality among spinal locomotor ...
https://journals.physiology.org/doi/pdf/10.1152/jn.00338.2020
tdTomato (bottom half) from four representative animals. Red areas represent higher ... The half-life of synaptic proteins can be on the.
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53 Generation of Arabidopsis lines with a red fluorescent marker ...
https://www.tandfonline.com/doi/full/10.1080/15592324.2020.1790196
Additionally, transient assays showed that tdTomato-CTT can also be used as an ER marker in onion, rice, and Nicotiana benthamiana. We believe that TA proteins ...
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54 New Generations of MS2 Variants and MCP Fusions to Detect ...
https://hal.archives-ouvertes.fr/hal-03044742/document
proteins (FP, i.e. eGFP, mCherry, tdTomato, HALO, photoactivatable ... This allowed measuring the half-life of rapidly decaying mRNAs.
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55 Early-onset impairment of the ubiquitin-proteasome system in ...
https://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-020-0894-0
The UbG76V-GFP and TdTomato open reading frames were expressed under ... shorter half-life (~ 55 min) than non-phosphorylated α-synuclein ...
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56 Generation of Bacterial Artificial Chromosome (BAC) transgenes
https://www.immunology.uzh.ch/aboutus/miscellaneous/genetargeting/bactransgenes.html
tdTomato-pest 2A Cre frt Kan(R) frt, short half life red color + Cre. CreDo and CreAc frt Kan(R) frt, Cre expression in dual marker populations.
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57 Highlighted clients publications with our models - genOway
https://www.genoway.com/science-techno/scientific-publications/paper/highlights.htm
Small molecules can indeed provide increased oral bioavailability, bio-efficiency and short half-life activity, which is particularly relevant for IRAEs.
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58 Nuclear membrane ruptures underlie the vascular pathology ...
https://insight.jci.org/articles/view/151515
(A) Expression of Nuc-tdTomato in SMCs is uniform in the ascending aorta. ... lamin B1 has a very long half-life in mouse tissues (41, 42).
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59 Science Journals — AAAS - Squarespace
https://static1.squarespace.com/static/592f089eebbd1a8c7a84f0a0/t/5ee77320c6fcc20e01b5954d/1592226598222/Science+Adv.+2020.pdf
cells were sorted into tdTomato-negative (D2 2C−) and tdTomato- ... mRNA half-life of Zscan4d, a downstream target of Dux, is not sig-.
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60 Characterization of the human GnRH neuron developmental ...
https://researchportal.helsinki.fi/files/133875552/dmm040105.full.pdf
transcriptome using a GNRH1-TdTomato reporter line in ... Approximately half of the patients can be diagnosed at.
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61 Catchup: a mouse model for imaging-based tracking and ...
http://www.immunodynamics.de/wp-content/uploads/2015/03/nmeth.3322.pdf
fluorescent protein tdtomato, we generated a mouse model ... the short half-life of neutrophils after the onset of Ly6G expres-.
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62 Fluorescent Protein Engineering References - Zeiss Campus
https://zeiss-campus.magnet.fsu.edu/referencelibrary/fluorescentproteins/fpengineering.html
Nathan Shaner and associates describe mCherry, mOrange, tdTomato, mStrawberry and several additional ... and a 40-fold increase in photobleaching half-life.
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63 Vascular Endothelial Senescence - ReadCube
https://www.readcube.com/articles/10.3389/fphys.2021.693067
... endothelial cellular senescence through lifestyle and pharmacological interventions ... (3) the half-life of senescent cells are about2.6–4.2 months for ...
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64 Why Do Some Vaccines Create Long-Lived Responses and ...
https://www.technologynetworks.com/vaccines/news/why-do-some-vaccines-create-long-lived-responses-and-others-dont-367833
... model expressed a fluorescent protein (called TdTomato protein). ... then decline at a rate of around 0.1% a day with a half life of ...
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65 Automated Live Cell Imaging for Quantification of Catalytic ...
https://www.nist.gov/programs-projects/automated-live-cell-imaging-quantification-catalytic-rates-individual-cells
Degradation for this reporter was analyzed as a first order rate process with a degradation half-life of 2.8 +/- 0.7 h, where the variance reflects the ...
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66 Stop3x trend - I Want The Winner
https://inno5.fr/stop3x-trend.html
... "it will change your life", "it will ruin your childhood". ... are designed for crossbows that utilize 20" arrows with a non-proprietary half moon nock.
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67 FlyBase Experimental Tool Report: tdTomato
https://flybase.org/reports/FBto0000121
tdTomato is a red fluorescent protein with an excitation peak of 554nm and an emission peak of 581nm in vitro. It is an artificial derivative of the ...
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68 A guide to choosing fluorescent proteins - Abcam
https://www.abcam.com/content/choosing-a-fluorescent-protein
T-sapphire has a bleaching half-life (t½; time for an initial emission rate of x photons/s to reduce to half) of 25 seconds, but EGFP is much more stable, ...
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69 Principles of Bone Biology - Page 1889 - Google Books Result
https://books.google.com/books?id=_8CqDwAAQBAJ&pg=PA1889&lpg=PA1889&dq=half+life+tdtomato&source=bl&ots=qbmHAZURM9&sig=ACfU3U0FVb7CPBvm_D0yhP1aQR_mVzxmiQ&hl=en&sa=X&ved=2ahUKEwjc38TKm837AhWJYKQEHX8EB2YQ6AF6BQiJAhAD
Following Cre excision, ubiquitously expressed tdTomato is deleted and cells cease to ... The half-life of tamoxifen in vivo is reported to be around 16 h, ...
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70 The Biology and Therapeutic Application of Mesenchymal ...
https://books.google.com/books?id=qN5ODQAAQBAJ&pg=PA137&lpg=PA137&dq=half+life+tdtomato&source=bl&ots=WUAKT30gKU&sig=ACfU3U2ZOTFxYxbSrCROa-kBVjnb6IPepg&hl=en&sa=X&ved=2ahUKEwjc38TKm837AhWJYKQEHX8EB2YQ6AF6BQiKAhAD
... they are not without issues owing to the short half-life of the reagent [147], ... tdTomato is an exceptionally bright red fluorescent protein - 6 times ...
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71 Molecular Imaging II - Page 191 - Google Books Result
https://books.google.com/books?id=f3IavWtPL4YC&pg=PA191&lpg=PA191&dq=half+life+tdtomato&source=bl&ots=OsboXSPTio&sig=ACfU3U34Lxov2O-Fzvi37e1X0XuzMe25NQ&hl=en&sa=X&ved=2ahUKEwjc38TKm837AhWJYKQEHX8EB2YQ6AF6BQiLAhAD
Purified proteins (from left : mHoneydew, mBanana, mOrange, tdTomato, mTangerine, ... 2000) and to the considerable short biological half-life of in and ...
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72 Brown Adipose Tissue - Page 47 - Google Books Result
https://books.google.com/books?id=NwGQDwAAQBAJ&pg=PA47&lpg=PA47&dq=half+life+tdtomato&source=bl&ots=b9zHlrDNX8&sig=ACfU3U2ruDnKu3dX5Ic0-0y_1X3CW-qEEA&hl=en&sa=X&ved=2ahUKEwjc38TKm837AhWJYKQEHX8EB2YQ6AF6BQiMAhAD
As preceding studies in non-mitotic mouse cells had shown, the half-life of eGFP ... they inserted the luciferase2-T2A-tdTomato transgene cassette at the ...
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73 Advances in Intravital Microscopy: From Basic to Clinical ...
https://books.google.com/books?id=IPhWBQAAQBAJ&pg=PA85&lpg=PA85&dq=half+life+tdtomato&source=bl&ots=iiYglluM30&sig=ACfU3U0OfcGnyxudH_K4ga3vvA5SZOLfXQ&hl=en&sa=X&ved=2ahUKEwjc38TKm837AhWJYKQEHX8EB2YQ6AF6BQj_ARAD
... range of fluorescent proteins (including eCFP, eYFP, eGFP, RFP, tdTomato). ... It should be noted, however, that the half-life of FPs may vary in vivo, ...
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74 The Role of Glia in Alzheimer's Disease
https://books.google.com/books?id=HsuNDwAAQBAJ&pg=PA110&lpg=PA110&dq=half+life+tdtomato&source=bl&ots=D4zSSkypKN&sig=ACfU3U2rCduvzdrWEG0ERvjzXzJjdFCscA&hl=en&sa=X&ved=2ahUKEwjc38TKm837AhWJYKQEHX8EB2YQ6AF6BQiIAhAD
... a rather deactivated process and that about half of the microglia persist until ... Since new tdTomato-expressing cells were reported to appear in the ...
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