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Google Keyword Rankings for : dnase stock solution recipe
1
DNase I Stock Solution (10 U/µL) - CSH Protocols
http://cshprotocols.cshlp.org/content/2019/4/pdb.rec105882.full?text_only=true
DNase I Stock Solution (10 U/µL) ; Dithiothreitol (1 m), 1 mm ; Glycerol (100%), 50% ; Combine the reagents listed above to prepare storage buffer. On ice, ...
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http://cshprotocols.cshlp.org/content/2019/4/pdb.rec105882.full?text_only=true
DNase I Stock Solution (10 U/µL) ; Dithiothreitol (1 m), 1 mm ; Glycerol (100%), 50% ; Combine the reagents listed above to prepare storage buffer. On ice, ...
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2
DNase 1 Stock Solution - GoldBio
https://goldbio.com/documents/3814/DNase+1+Stock+Solution.pdf
3. Add 500 µl glycerol and mix. 4. Store DNase I Stock Solution at -20°C. Avoid repeated freeze/thaw.
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https://goldbio.com/documents/3814/DNase+1+Stock+Solution.pdf
3. Add 500 µl glycerol and mix. 4. Store DNase I Stock Solution at -20°C. Avoid repeated freeze/thaw.
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3
DNase I Solution | Canvax
https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/09/DNase-I-solution.pdf
DNase I, RNase free recombinant, is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release.
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https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/09/DNase-I-solution.pdf
DNase I, RNase free recombinant, is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release.
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4
Can anyone suggest to me how to prepare DNase buffer?
https://www.researchgate.net/post/Can_anyone_suggest_to_me_how_to_prepare_DNase_buffer
The recipe of DNase buffer is: 100 mM Tris-HCl (pH 7.5), 25 mM MgCl2, 1 mM CaCl2. You can make it in DEPC treated water to get rid of RNase.
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https://www.researchgate.net/post/Can_anyone_suggest_to_me_how_to_prepare_DNase_buffer
The recipe of DNase buffer is: 100 mM Tris-HCl (pH 7.5), 25 mM MgCl2, 1 mM CaCl2. You can make it in DEPC treated water to get rid of RNase.
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5
DNase I Treatment Version 1 Bonnie Poulos, Tucson Marine ...
https://cpb-us-w2.wpmucdn.com/u.osu.edu/dist/e/20087/files/2015/08/DNase_I_Treatment_v1-20c6obw.pdf
DNase I was tested for ability to degrade DNA in solutions containing iron ... DNase I made up to 40,000 U/ml (-20oC freezer door) = stock solution.
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https://cpb-us-w2.wpmucdn.com/u.osu.edu/dist/e/20087/files/2015/08/DNase_I_Treatment_v1-20c6obw.pdf
DNase I was tested for ability to degrade DNA in solutions containing iron ... DNase I made up to 40,000 U/ml (-20oC freezer door) = stock solution.
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6
DNase I Solution (2500 U/mL) - Thermo Fisher Scientific
https://www.thermofisher.com/order/catalog/product/90083
Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein extraction efficiency.Features of Thermo Scientific DNase I: Degrades ...
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https://www.thermofisher.com/order/catalog/product/90083
Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein extraction efficiency.Features of Thermo Scientific DNase I: Degrades ...
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7
Preparation of DNase-free Bovine Pancreatic RNase A
https://www.laboratorynotes.com/preparation-of-dnase-free-bovine-pancreatic-rnase-a/
› preparation-of-dnas...
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https://www.laboratorynotes.com/preparation-of-dnase-free-bovine-pancreatic-rnase-a/
› preparation-of-dnas...
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8
Directions for Use DNase I - VWR
https://de.vwr.com/assetsvc/asset/de_DE/id/18501571/contents
VWR Life Science AMRESCO's DNase I is an RNase-free endonuclease originating from ... Concentration of stock solutions will vary depending on application.
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https://de.vwr.com/assetsvc/asset/de_DE/id/18501571/contents
VWR Life Science AMRESCO's DNase I is an RNase-free endonuclease originating from ... Concentration of stock solutions will vary depending on application.
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9
An efficient method to eliminate the protease activity ... - PubMed
https://pubmed.ncbi.nlm.nih.gov/25944416/
Bio Basic DNase stock solution (in Tris-HCl buffer [pH 8.0] containing 5mM CaCl2) was first incubated at 50 °C to generate autolysis of ...
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https://pubmed.ncbi.nlm.nih.gov/25944416/
Bio Basic DNase stock solution (in Tris-HCl buffer [pH 8.0] containing 5mM CaCl2) was first incubated at 50 °C to generate autolysis of ...
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10
RNase-Free DNase Set Product Sheet - QIAGEN
https://www.qiagen.com/kr/resources/download.aspx?id=b0ca9e5a-ff87-476e-811b-ff80e4f07b3f&lang=en
For DNase treatment with QIAGEN® or PreAnalytiX™ RNA purification kits ... Prepare DNase I stock solution before using the RNase-free DNase Set for the ...
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https://www.qiagen.com/kr/resources/download.aspx?id=b0ca9e5a-ff87-476e-811b-ff80e4f07b3f&lang=en
For DNase treatment with QIAGEN® or PreAnalytiX™ RNA purification kits ... Prepare DNase I stock solution before using the RNase-free DNase Set for the ...
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11
Nucleic acid preparation - INTERCHIM
https://www.interchim.fr/ft/B/BD017A.pdf
DNase I. Dissolving the entire 10 000 units vial in 5 ml, or the entire ... highly soluble (> 50 mg/ml) powder or as a 20 mg/ml liquid stock solution, each.
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https://www.interchim.fr/ft/B/BD017A.pdf
DNase I. Dissolving the entire 10 000 units vial in 5 ml, or the entire ... highly soluble (> 50 mg/ml) powder or as a 20 mg/ml liquid stock solution, each.
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12
RNase-Free DNase I
http://net.bangong.cn:6109/upload/file/20200722/20200722180345_19561.pdf
column DNA digestion. Those buffer may affect the combination of RNA and membrane, which lead to low. RNA yield. 1. Preparation of DNase I stock solution: ...
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http://net.bangong.cn:6109/upload/file/20200722/20200722180345_19561.pdf
column DNA digestion. Those buffer may affect the combination of RNA and membrane, which lead to low. RNA yield. 1. Preparation of DNase I stock solution: ...
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13
Dnase I Solution at Thomas Scientific
https://www.thomassci.com/scientific-supplies/Dnase-I-Solution
…this application, DNase free RNase A is used at a final concentration of 10 mg/mL. Boiling stock solutions of this RNase A product to inactivate residual ...
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https://www.thomassci.com/scientific-supplies/Dnase-I-Solution
…this application, DNase free RNase A is used at a final concentration of 10 mg/mL. Boiling stock solutions of this RNase A product to inactivate residual ...
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14
DNase I (RNase-free) - NEB
https://www.neb.com/products/m0303-dnase-i-rnase-free
DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and ...
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https://www.neb.com/products/m0303-dnase-i-rnase-free
DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and ...
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15
Deoxyribonuclease I - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/deoxyribonuclease-i
Deoxyribonuclease I (DNase I) is an endonuclease which is secreted to cleave ... which will affect the solvent volumes required to prepare stock solutions.
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https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/deoxyribonuclease-i
Deoxyribonuclease I (DNase I) is an endonuclease which is secreted to cleave ... which will affect the solvent volumes required to prepare stock solutions.
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16
RNase-Free DNase I Set(DN050).ai
https://download.rbcbioscience.com/RBC%20Real%20Genomics/Purification%20Enzymes/DNase%20I%20%20%28RNase-free%29/RNase-Free%20DNase%20I%20Set%28DN050%29.pdf
Preparation before using. Prepare DNase I stock solution before using the RNase-Free DNase Set for the first time. Dissolve the lyophilized DNase I (1500 ...
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https://download.rbcbioscience.com/RBC%20Real%20Genomics/Purification%20Enzymes/DNase%20I%20%20%28RNase-free%29/RNase-Free%20DNase%20I%20Set%28DN050%29.pdf
Preparation before using. Prepare DNase I stock solution before using the RNase-Free DNase Set for the first time. Dissolve the lyophilized DNase I (1500 ...
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17
ROCHE DNAse I Rec. Grade I | 4536282001 | SIGMA ALDRICH
https://www.scientificlabs.co.uk/product/4536282001
Store this solution at -15 to-25 °C; it will not freeze at this temperature and will be stable for several weeks.Buffer 2: 20 mM Tris-HCl, 50 mM NaCl, 1 mM ...
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https://www.scientificlabs.co.uk/product/4536282001
Store this solution at -15 to-25 °C; it will not freeze at this temperature and will be stable for several weeks.Buffer 2: 20 mM Tris-HCl, 50 mM NaCl, 1 mM ...
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18
Laboratory Stock Solutions and Equipment - 1998
https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/0471143030.cba02as00
SDS sample buffer ; 0.5 M Tris⋅Cl, pH 6.8a · See recipe below. 2.5 ml. 5.0 ml ; SDS. 0.4 g. 0.8 g ; Glycerol. 2.0 ml. 4.0 ml ; Bromphenol blue. 20 ...
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https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/0471143030.cba02as00
SDS sample buffer ; 0.5 M Tris⋅Cl, pH 6.8a · See recipe below. 2.5 ml. 5.0 ml ; SDS. 0.4 g. 0.8 g ; Glycerol. 2.0 ml. 4.0 ml ; Bromphenol blue. 20 ...
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19
Blueprint: DNase I-‐‑seq for Human hematopoietic cells
https://www.blueprint-epigenome.eu/UserFiles/File/Protocols/Blueprint_DNase1_Protocol.pdf
Blueprint: DNase I-‐‑seq for Human hematopoietic cells ... Nuclei Preparation: ... Stock. Final concentration. Volume stock solution.
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https://www.blueprint-epigenome.eu/UserFiles/File/Protocols/Blueprint_DNase1_Protocol.pdf
Blueprint: DNase I-‐‑seq for Human hematopoietic cells ... Nuclei Preparation: ... Stock. Final concentration. Volume stock solution.
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20
RNase-Free DNase I - TIANGEN
https://en.tiangen.com/upload/file/20201027/20201027135103_81150.pdf
Preparation of DNase I stock solution: Dissolve the lyophilized. DNase I (1500 units) in 550 μl of the RNase-free ddH2O. Mix gently by inverting ...
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https://en.tiangen.com/upload/file/20201027/20201027135103_81150.pdf
Preparation of DNase I stock solution: Dissolve the lyophilized. DNase I (1500 units) in 550 μl of the RNase-free ddH2O. Mix gently by inverting ...
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21
Omni-ATAC – An improved and broadly applicable
https://med.stanford.edu/content/dam/sm/cpdr/documents/changlab_Omni-ATAC_Protocol.pdf
remove DNase prior to proceeding to ATAC-seq transposition reaction. ... Detergents - All detergents are resuspended as 100x stock solutions.
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https://med.stanford.edu/content/dam/sm/cpdr/documents/changlab_Omni-ATAC_Protocol.pdf
remove DNase prior to proceeding to ATAC-seq transposition reaction. ... Detergents - All detergents are resuspended as 100x stock solutions.
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22
Disaggregation of Clumped Cells | Flow Cytometry
https://medicine.uiowa.edu/flowcytometry/disaggregation-clumped-cells
Add 0.02mg/ml DNase I (type IIS) to all cell preparation steps, including wash steps, to eliminate free DNA from broken cells that leads to aggregation.
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https://medicine.uiowa.edu/flowcytometry/disaggregation-clumped-cells
Add 0.02mg/ml DNase I (type IIS) to all cell preparation steps, including wash steps, to eliminate free DNA from broken cells that leads to aggregation.
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23
OmniPur® DNase I, Bovine Pancreas - Calbiochem | 3750-OP
https://www.emdmillipore.com/US/en/product/OmniPur-DNase-I-Bovine-Pancreas-Calbiochem,EMD_BIO-3750-OP
OmniPur® DNase I, Bovine Pancreas - Calbiochem - Find MSDS or SDS, a COA, ... enzyme to a concentration of at least 2 mg/ml in a 50% Glycerol solution (Cat.
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https://www.emdmillipore.com/US/en/product/OmniPur-DNase-I-Bovine-Pancreas-Calbiochem,EMD_BIO-3750-OP
OmniPur® DNase I, Bovine Pancreas - Calbiochem - Find MSDS or SDS, a COA, ... enzyme to a concentration of at least 2 mg/ml in a 50% Glycerol solution (Cat.
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24
Tribo™ DNase I Solution (1 mg/mL) - Tribioscience
https://tribioscience.com/product/life-sciences/nucleotide/tribo-dnase-i-solution-1-mg-ml/
The DNase I Solution is provided at a concentration of 1 mg/mL (2000U/mL). 10x Reaction Buffer and Stop Solution are included in the kit.
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https://tribioscience.com/product/life-sciences/nucleotide/tribo-dnase-i-solution-1-mg-ml/
The DNase I Solution is provided at a concentration of 1 mg/mL (2000U/mL). 10x Reaction Buffer and Stop Solution are included in the kit.
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25
Western blot sample preparation - Abcam
https://www.abcam.com/protocols/sample-preparation-for-western-blot
Preparation of samples for loading into gels · 4% SDS · 10% 2-mercaptoethanol · 20% glycerol · 0.004% bromophenol blue · 0.125 M Tris HCl · Check the pH and bring ...
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https://www.abcam.com/protocols/sample-preparation-for-western-blot
Preparation of samples for loading into gels · 4% SDS · 10% 2-mercaptoethanol · 20% glycerol · 0.004% bromophenol blue · 0.125 M Tris HCl · Check the pH and bring ...
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26
DNase I functional microgels for neutrophil extracellular trap ...
https://pubs.rsc.org/en/content/articlehtml/2022/bm/d1bm01591e
4.7 mL of the DNase I solution was directly added to 8 mg of p(HPMA-co-CBMAA) microgel. 1 mg mL−1 stock solutions of EDC (6.4 mM) and sulfo-NHS ...
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https://pubs.rsc.org/en/content/articlehtml/2022/bm/d1bm01591e
4.7 mL of the DNase I solution was directly added to 8 mg of p(HPMA-co-CBMAA) microgel. 1 mg mL−1 stock solutions of EDC (6.4 mM) and sulfo-NHS ...
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27
Horizontal Electrophoresis - Bio-Rad
https://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6205.pdf
Recipes for TAE and TBE Electrophoresis Buffers. Agarose gels are generally run two types of ... TAE is often prepared in concentrated stock solutions of.
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https://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6205.pdf
Recipes for TAE and TBE Electrophoresis Buffers. Agarose gels are generally run two types of ... TAE is often prepared in concentrated stock solutions of.
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28
RNase A (DNase free) - Jena Bioscience
https://www.jenabioscience.com/images/PDF/EN-179.0002.pdf
Additional Storage Conditions: stock solutions in TE buffer should be aliquoted and stored at -20 °C ... Plasmid and genomic DNA preparation.
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https://www.jenabioscience.com/images/PDF/EN-179.0002.pdf
Additional Storage Conditions: stock solutions in TE buffer should be aliquoted and stored at -20 °C ... Plasmid and genomic DNA preparation.
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29
Protocol II: 1 M Tris-HCl Buffer Stock Solution (1 liter) - NEB
https://www.nebiolabs.com.au/protocols/0001/01/01/protocol-ii-1-m-tris-hcl-buffer-stock-solution-1-liter
Protocol · Solution A: Dissolve 121.14 g Tris (American Bioanalytical #AB14042) in 800 ml dH2O. · Adjust pH to 7.0 with the appropriate volume of concentrated HCl ...
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https://www.nebiolabs.com.au/protocols/0001/01/01/protocol-ii-1-m-tris-hcl-buffer-stock-solution-1-liter
Protocol · Solution A: Dissolve 121.14 g Tris (American Bioanalytical #AB14042) in 800 ml dH2O. · Adjust pH to 7.0 with the appropriate volume of concentrated HCl ...
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30
Thermo Scientific™ DNase I Solution (1 Unit/µL), RNase-free
https://laboshop.ae/product/thermo-scientific-dnase-i-solution-1-unitl-rnase-free559
Kits & Chemicals > Enzymes and Inhibitors & Protein Preparation and ... Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein ...
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https://laboshop.ae/product/thermo-scientific-dnase-i-solution-1-unitl-rnase-free559
Kits & Chemicals > Enzymes and Inhibitors & Protein Preparation and ... Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein ...
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31
PROTOCOL FOR GISH GENOMIC IN SITU HYBRIDIZATION
https://www.k-state.edu/wgrc/images/pdfs/GISH.pdf
5 M NaCl. Dissolve 29.22 g of NaCl in ddH2O to a final volume of 100 ml. STOCK SOLUTION RECIPES. Page 3. 3. G E N O M I C I N S I T U.
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https://www.k-state.edu/wgrc/images/pdfs/GISH.pdf
5 M NaCl. Dissolve 29.22 g of NaCl in ddH2O to a final volume of 100 ml. STOCK SOLUTION RECIPES. Page 3. 3. G E N O M I C I N S I T U.
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32
Buffers and solutions - Integrated DNA Technologies
https://www.idtdna.com/pages/products/reagents-and-kits/buffers-and-solutions
Nuclease Free Water. For initial resuspension and storage of single-stranded RNA oligos or making dilute solutions from stock ; Nuclease-Free Duplex Buffer. For ...
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https://www.idtdna.com/pages/products/reagents-and-kits/buffers-and-solutions
Nuclease Free Water. For initial resuspension and storage of single-stranded RNA oligos or making dilute solutions from stock ; Nuclease-Free Duplex Buffer. For ...
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33
Guidelines for the Cell Sorting - The Rockefeller University
https://www.rockefeller.edu/fcrc/sortprep/
Use all applicable Sample Preparation Tips; If you have extensive cell death in the sample, add DNAse (see Buffer Suggestions). It will not only decrease ...
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https://www.rockefeller.edu/fcrc/sortprep/
Use all applicable Sample Preparation Tips; If you have extensive cell death in the sample, add DNAse (see Buffer Suggestions). It will not only decrease ...
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34
The essential role of Ca2+ in the activity of bovine pancreatic ...
https://www.jbc.org/article/S0021-9258(19)41672-4/pdf
The values measured for calcium concentration were not affected by DNase or by DNA at the concentrations present in the assay solutions. RESULTS. Requirememl of ...
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https://www.jbc.org/article/S0021-9258(19)41672-4/pdf
The values measured for calcium concentration were not affected by DNase or by DNA at the concentrations present in the assay solutions. RESULTS. Requirememl of ...
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35
AAV Titration by qPCR Using SYBR Green Technology
https://www.addgene.org/protocols/aav-titration-qpcr-using-sybr-green-technology/
Procedure · 5μL sample + 39μL H2O + 5μL 10x DNase buffer + 1μL DNase · Gently mix sample (do not vortex) · Incubate 30 minutes at 37°C · Transfer to ...
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https://www.addgene.org/protocols/aav-titration-qpcr-using-sybr-green-technology/
Procedure · 5μL sample + 39μL H2O + 5μL 10x DNase buffer + 1μL DNase · Gently mix sample (do not vortex) · Incubate 30 minutes at 37°C · Transfer to ...
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36
Actin Is the Naturally Occurring Inhibitor of Deoxyribonuclease I
https://www.pnas.org/doi/pdf/10.1073/pnas.71.12.4742
and for the preparation of DNase-agarose (see below). DNase. I inhibitor from calf thymus was purified as ... A stock solution of purified actin was.
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https://www.pnas.org/doi/pdf/10.1073/pnas.71.12.4742
and for the preparation of DNase-agarose (see below). DNase. I inhibitor from calf thymus was purified as ... A stock solution of purified actin was.
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37
Solved I am provided with the following stock solutions: 1M
https://www.chegg.com/homework-help/questions-and-answers/provided-following-stock-solutions-1m-tris-ph-8-5-m-nacl-1m-mgcl2-lysozyme-dnase-prepare-2-q23806320
Prepare a 25 mL solution of complete lysis buffer using the following recipe: - 50 mM Tris (pH 8) - 150 mM NaCl - 2 mM MgCl2 - 0.5 mg/mL lysozyme - 0.04 uL/mL ...
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https://www.chegg.com/homework-help/questions-and-answers/provided-following-stock-solutions-1m-tris-ph-8-5-m-nacl-1m-mgcl2-lysozyme-dnase-prepare-2-q23806320
Prepare a 25 mL solution of complete lysis buffer using the following recipe: - 50 mM Tris (pH 8) - 150 mM NaCl - 2 mM MgCl2 - 0.5 mg/mL lysozyme - 0.04 uL/mL ...
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38
Preparation of Nuclear and Cytoplasmic Extracts from ...
https://www.med.upenn.edu/robertsonlab/assets/user-content/documents/Preparation%20of%20Nuclear%20and%20Cytoplasmic%20Extracts%20from%20Mammalian%20Cells.pdf
the preparation of the cytoplasmic (S-100) fraction. ... DNA-Protein ... PMSF is not stable in aqueous solution, and the stock should be added to solutions ...
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https://www.med.upenn.edu/robertsonlab/assets/user-content/documents/Preparation%20of%20Nuclear%20and%20Cytoplasmic%20Extracts%20from%20Mammalian%20Cells.pdf
the preparation of the cytoplasmic (S-100) fraction. ... DNA-Protein ... PMSF is not stable in aqueous solution, and the stock should be added to solutions ...
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39
How to make a primer 100 µM stock solution - YouTube
https://www.youtube.com/watch?v=8S7ZmSwQRhc
MrSimpleScience
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https://www.youtube.com/watch?v=8S7ZmSwQRhc
MrSimpleScience
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40
Tuveson Laboratory Murine and Human Organoid Protocols
https://tuvesonlab.labsites.cshl.edu/wp-content/uploads/sites/49/2018/06/20170523_OrganoidProtocols.pdf
Organoid Preparation and Culturing Protocols, page 2 ... The DNAse stock sometimes comes out of solution, so the tube should be mixed just before.
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https://tuvesonlab.labsites.cshl.edu/wp-content/uploads/sites/49/2018/06/20170523_OrganoidProtocols.pdf
Organoid Preparation and Culturing Protocols, page 2 ... The DNAse stock sometimes comes out of solution, so the tube should be mixed just before.
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41
To make Glycerol Stocks of Plasmids
http://www.people.vcu.edu/~pli/Protocols/Plasmid%20Preparation.pdf
To be done in the hood and use RNase/DNase free tips** ... Glycerol 60% stock solution ... Next antibiotic preparation of stock solution is as follows:.
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http://www.people.vcu.edu/~pli/Protocols/Plasmid%20Preparation.pdf
To be done in the hood and use RNase/DNase free tips** ... Glycerol 60% stock solution ... Next antibiotic preparation of stock solution is as follows:.
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42
Intracellular Cytokine Staining Procedure < Yale Flow Cytometry
https://medicine.yale.edu/immuno/flowcore/protocols/cytokines/
Stocks Ionomycin - 2.5 mg/ml in DMSO - stored -80°C 10µl stock — 40µl Clone ... Isolation/Preparation of Spleen Cells ... Resuspend in 1ml DNAse Solution.
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https://medicine.yale.edu/immuno/flowcore/protocols/cytokines/
Stocks Ionomycin - 2.5 mg/ml in DMSO - stored -80°C 10µl stock — 40µl Clone ... Isolation/Preparation of Spleen Cells ... Resuspend in 1ml DNAse Solution.
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43
Can I add enzymes (such as DNase) or other chemical ...
https://kb.10xgenomics.com/hc/en-us/articles/115001834226-Can-I-add-enzymes-such-as-DNase-or-other-chemical-additives-to-my-cell-suspension-
Answer: The use of collagenase, dispase, accutase, DNase I, or TurboNuclease during sample preparation in the Single Cell 3' or 5' assay have not been ...
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https://kb.10xgenomics.com/hc/en-us/articles/115001834226-Can-I-add-enzymes-such-as-DNase-or-other-chemical-additives-to-my-cell-suspension-
Answer: The use of collagenase, dispase, accutase, DNase I, or TurboNuclease during sample preparation in the Single Cell 3' or 5' assay have not been ...
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44
RNeasy® Mini Handbook
http://www.bea.ki.se/documents/EN-RNeasy%20handbook.pdf
Appendix E: DNase Digestion of RNA before RNA Cleanup ... If performing optional on-column DNase digestion, prepare DNase I stock solution.
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http://www.bea.ki.se/documents/EN-RNeasy%20handbook.pdf
Appendix E: DNase Digestion of RNA before RNA Cleanup ... If performing optional on-column DNase digestion, prepare DNase I stock solution.
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45
ISOLATE II RNA Mini Kit Product Manual - Bioline
https://www.bioline.com/mwdownloads/download/link/id/885/isolate_ii_rna_mini_kit_product_manual.pdf
is removed by an on-column DNase I digestion during the preparation (RNase-free DNase I is supplied). ... Preparing DNase I (RNase-free) stock solution.
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https://www.bioline.com/mwdownloads/download/link/id/885/isolate_ii_rna_mini_kit_product_manual.pdf
is removed by an on-column DNase I digestion during the preparation (RNase-free DNase I is supplied). ... Preparing DNase I (RNase-free) stock solution.
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46
Nuclei Isolation for Single Cell ATAC Sequencing
https://assets.ctfassets.net/an68im79xiti/5g035d2ngCW1aB9DFqPphO/71445a59fb282ea273a866c26cb5d319/CG000169_DemonstratedProtocol_NucleiIsolation_ATAC_Sequencing_RevD.pdf
presented in this protocol for nuclei sample preparation are critical for optimal Chromium ... Miltenyi Biotec MACS BSA Stock Solution. 130-091-376. Bel-Art.
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https://assets.ctfassets.net/an68im79xiti/5g035d2ngCW1aB9DFqPphO/71445a59fb282ea273a866c26cb5d319/CG000169_DemonstratedProtocol_NucleiIsolation_ATAC_Sequencing_RevD.pdf
presented in this protocol for nuclei sample preparation are critical for optimal Chromium ... Miltenyi Biotec MACS BSA Stock Solution. 130-091-376. Bel-Art.
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47
Primary Cortical Neuron Culture--Dissociated via Trypsin
https://www.hopkinsmedicine.org/institute_basic_biomedical_sciences/research_centers/metabolism_obesity_research/protocols/pdfs/a1a_primary_cortical_neuron_culture.pdf
o DNase o cFBS o Preparation media (for washes, trituration, etc…) ... o Dilute the 20 mL stock to make a final concentration of 0.5 x 106 cells/mL (50% ...
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https://www.hopkinsmedicine.org/institute_basic_biomedical_sciences/research_centers/metabolism_obesity_research/protocols/pdfs/a1a_primary_cortical_neuron_culture.pdf
o DNase o cFBS o Preparation media (for washes, trituration, etc…) ... o Dilute the 20 mL stock to make a final concentration of 0.5 x 106 cells/mL (50% ...
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48
Cresol Red Solution Preparation and Recipe - AAT Bioquest
https://www.aatbio.com/resources/buffer-preparations-and-recipes/cresol-red-solution
Prepare 800 mL of distilled water in a suitable container. Add 4000 g of sodium salt of cresol red (Aldrich) to the solution. Add distilled water until the ...
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https://www.aatbio.com/resources/buffer-preparations-and-recipes/cresol-red-solution
Prepare 800 mL of distilled water in a suitable container. Add 4000 g of sodium salt of cresol red (Aldrich) to the solution. Add distilled water until the ...
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49
DNase Agar - Neogen
https://www.neogen.com/categories/microbiology/dnase-agar/
Item No. NCM0161A ; Liters per 500 g, 13.00 ; Plates per 500 g, 700.00 ; Preparation. Suspend 42 g of the medium in one liter of purified water. Heat with frequent ...
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https://www.neogen.com/categories/microbiology/dnase-agar/
Item No. NCM0161A ; Liters per 500 g, 13.00 ; Plates per 500 g, 700.00 ; Preparation. Suspend 42 g of the medium in one liter of purified water. Heat with frequent ...
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50
CELL AND TISSUE LYSATE PREPARATION - Proteintech
https://www.ptglab.com/media/1636/1503366_protocols-for-web_lysate-preparation.pdf
The addition of DNase for DNA digestion is not recommended as this introduces protein contamination from the enzyme. b. Centrifuge at 10,000 x g (approximately ...
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https://www.ptglab.com/media/1636/1503366_protocols-for-web_lysate-preparation.pdf
The addition of DNase for DNA digestion is not recommended as this introduces protein contamination from the enzyme. b. Centrifuge at 10,000 x g (approximately ...
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51
Apoptosis, DNA Damage and Cell Proliferation Kit
https://www.bdbiosciences.com/en-us/products/reagents/functional-cell-based-reagents/apoptosis-dna-damage-and-cell-proliferation-kit.562253
(1) Recipe for Freezing Media: 10% dimethyl sulfoxide (DMSO) + 90% ... Note: DNase stock solution (ie, at 1 mg DNase/ml) may be refrozen once before it ...
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https://www.bdbiosciences.com/en-us/products/reagents/functional-cell-based-reagents/apoptosis-dna-damage-and-cell-proliferation-kit.562253
(1) Recipe for Freezing Media: 10% dimethyl sulfoxide (DMSO) + 90% ... Note: DNase stock solution (ie, at 1 mg DNase/ml) may be refrozen once before it ...
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52
TE buffer - Wikipedia
https://en.wikipedia.org/wiki/TE_buffer
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: ...
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https://en.wikipedia.org/wiki/TE_buffer
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: ...
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53
Plant Genomic DNA Extraction using CTAB
https://agriculture.uq.edu.au/files/5650/plant-genomic-dna-extraction-by-ctab-2-fiona.pdf
Proceed immediately to the DNA preparation protocol. DNA Preparation. 1. Add 5 ml of Buffer AP1 (preheated to 65°C) and 10 µl of RNase A stock solution.
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https://agriculture.uq.edu.au/files/5650/plant-genomic-dna-extraction-by-ctab-2-fiona.pdf
Proceed immediately to the DNA preparation protocol. DNA Preparation. 1. Add 5 ml of Buffer AP1 (preheated to 65°C) and 10 µl of RNase A stock solution.
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54
Make your own nuclease/nucleic acid decontaminating solution
https://pipettejockey.com/2016/05/06/make-your-own-nucleasenucleic-acid-decontaminating-solution/
MSDSs are the next best source for a recipe, however something to keep in mind is that the laws vary by locality and time as to what the ...
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https://pipettejockey.com/2016/05/06/make-your-own-nucleasenucleic-acid-decontaminating-solution/
MSDSs are the next best source for a recipe, however something to keep in mind is that the laws vary by locality and time as to what the ...
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55
Tissue Disaggregation
https://ccp.bwh.harvard.edu/wp-content/uploads/2021/10/Tissue-dissociation-protocol.pdf
CryoStor CD10 (BioLife Solutions, 210102) ... DNase I (Roche, 10-104-159-001, ... Add 10uL DNAse I (50mg/mL stock) to each C tube for final 100ug/mL.
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https://ccp.bwh.harvard.edu/wp-content/uploads/2021/10/Tissue-dissociation-protocol.pdf
CryoStor CD10 (BioLife Solutions, 210102) ... DNase I (Roche, 10-104-159-001, ... Add 10uL DNAse I (50mg/mL stock) to each C tube for final 100ug/mL.
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56
DNase I Solution (1 mg/mL) for Cell Dissociation
https://www.stemcell.com/products/dnase-i-solution-1-mg-ml.html
Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful to reduce or prevent the clumping of concentrated and/or cryopreserved cell suspensions following ...
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https://www.stemcell.com/products/dnase-i-solution-1-mg-ml.html
Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful to reduce or prevent the clumping of concentrated and/or cryopreserved cell suspensions following ...
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57
DATA SHEET
http://gentaurpdf.com/pdf/DNase%20I.pdf
Recommended DNase I Solution Preparation and Storage ... stock enzyme is recommended to avoid repeated freeze/thaw cycles. The. DNase I stock enzyme can be ...
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http://gentaurpdf.com/pdf/DNase%20I.pdf
Recommended DNase I Solution Preparation and Storage ... stock enzyme is recommended to avoid repeated freeze/thaw cycles. The. DNase I stock enzyme can be ...
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58
DNAse I qualification and sample treatment - Molecular Devices
https://www.moleculardevices.com/en/assets/app-note/reagents/dna-dnase-i-qualification-and-sample-treatment
Three levels of DNase I are recommend for the initial testing: 25, 50 and 100 activity units (U). Step 1 Prepare a stock solution of DNase I at 2500 U/mL in the ...
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https://www.moleculardevices.com/en/assets/app-note/reagents/dna-dnase-i-qualification-and-sample-treatment
Three levels of DNase I are recommend for the initial testing: 25, 50 and 100 activity units (U). Step 1 Prepare a stock solution of DNase I at 2500 U/mL in the ...
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59
Preparation of DNase Aliquots 1.0 Purpose SOP-BCR-4.23 ...
https://www.med.umich.edu/wicha-lab/SOP/SOP%204.23-Preparation%20of%20DNase.pdf
SOP-BCR-4.23 details the preparation of 1mg/ml DNase Aliquots. 2.0. Scope. BCR-SOP-4.23 covers all personnel, resources and equipment in the ...
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https://www.med.umich.edu/wicha-lab/SOP/SOP%204.23-Preparation%20of%20DNase.pdf
SOP-BCR-4.23 details the preparation of 1mg/ml DNase Aliquots. 2.0. Scope. BCR-SOP-4.23 covers all personnel, resources and equipment in the ...
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60
Thermo Scientific™ DNase I Solution (1 unit/µL), RNase-free
https://www.fishersci.pt/shop/products/thermo-scientific-pierce-dnase-i/15198325
Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve ... In Stock Log in to see stock. Add to basket. This item is not returnable.
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https://www.fishersci.pt/shop/products/thermo-scientific-pierce-dnase-i/15198325
Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve ... In Stock Log in to see stock. Add to basket. This item is not returnable.
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61
RQ1 RNase-Free DNase - Promega Corporation
https://www.promega.com/products/cloning-and-dna-markers/molecular-biology-enzymes-and-reagents/rq1-rnase_free-dnase/
RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl ...
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https://www.promega.com/products/cloning-and-dna-markers/molecular-biology-enzymes-and-reagents/rq1-rnase_free-dnase/
RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl ...
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62
DNase I treatment of virus supernatant.
https://www.ucl.ac.uk/towers-lab/sites/towers_lab/files/removing_plasmid_dna_from_virus_preps_by_dnase.pdf
We use our reconstituted stock DNase I, Lyophilized bovine pancreas 6.110 ... purified RQ1 DNase (Promega)is used, 20 Units/ml of viral supernatant is.
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https://www.ucl.ac.uk/towers-lab/sites/towers_lab/files/removing_plasmid_dna_from_virus_preps_by_dnase.pdf
We use our reconstituted stock DNase I, Lyophilized bovine pancreas 6.110 ... purified RQ1 DNase (Promega)is used, 20 Units/ml of viral supernatant is.
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63
Alcohol: Methods and Protocols - Page 218 - Google Books Result
https://books.google.com/books?id=NHXYLVDfPBsC&pg=PA218&lpg=PA218&dq=dnase+stock+solution+recipe&source=bl&ots=wuqRCSwMlr&sig=ACfU3U2xudNDgX3klA1Y81GPvjMjKFkIeg&hl=en&sa=X&ved=2ahUKEwjTq-zZwc77AhUpmWoFHZunC7sQ6AF6BQiDAhAD
BSS (See recipe in Subheading 2.1.2.). 2. Liberase blendzyme 3 (Roche ... Stock DNase solution: dissolve DNase in RPMI at 4000 U/mL (see Note 1). c.
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https://books.google.com/books?id=NHXYLVDfPBsC&pg=PA218&lpg=PA218&dq=dnase+stock+solution+recipe&source=bl&ots=wuqRCSwMlr&sig=ACfU3U2xudNDgX3klA1Y81GPvjMjKFkIeg&hl=en&sa=X&ved=2ahUKEwjTq-zZwc77AhUpmWoFHZunC7sQ6AF6BQiDAhAD
BSS (See recipe in Subheading 2.1.2.). 2. Liberase blendzyme 3 (Roche ... Stock DNase solution: dissolve DNase in RPMI at 4000 U/mL (see Note 1). c.
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64
Molecular Biology Techniques: A Classroom Laboratory Manual
https://books.google.com/books?id=C4z_7Vd8EEsC&pg=PT223&lpg=PT223&dq=dnase+stock+solution+recipe&source=bl&ots=KEInFkZ1Ii&sig=ACfU3U2b1d-TqkoyGYmVnaGubVbyq7StaA&hl=en&sa=X&ved=2ahUKEwjTq-zZwc77AhUpmWoFHZunC7sQ6AF6BQiCAhAD
... 133–139 quantitative analysis after reverse transcription polymerase chain reaction, 141–144, 147–149 Chloronaphthol, stock solution preparation, 170 ...
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https://books.google.com/books?id=C4z_7Vd8EEsC&pg=PT223&lpg=PT223&dq=dnase+stock+solution+recipe&source=bl&ots=KEInFkZ1Ii&sig=ACfU3U2b1d-TqkoyGYmVnaGubVbyq7StaA&hl=en&sa=X&ved=2ahUKEwjTq-zZwc77AhUpmWoFHZunC7sQ6AF6BQiCAhAD
... 133–139 quantitative analysis after reverse transcription polymerase chain reaction, 141–144, 147–149 Chloronaphthol, stock solution preparation, 170 ...
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65
Culture of Cells for Tissue Engineering
https://books.google.com/books?id=KkcisIcCS_oC&pg=PA498&lpg=PA498&dq=dnase+stock+solution+recipe&source=bl&ots=2q2Z06mY8u&sig=ACfU3U13Fx54iNXWd3ltcgyTK1F2ZT32aw&hl=en&sa=X&ved=2ahUKEwjTq-zZwc77AhUpmWoFHZunC7sQ6AF6BQj1ARAD
DNase stock solution preparing for engineered heart tissues, 266 preparing for neuroendocrine/neuronal cell culture, 396 DNA standard solution, preparation ...
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https://books.google.com/books?id=KkcisIcCS_oC&pg=PA498&lpg=PA498&dq=dnase+stock+solution+recipe&source=bl&ots=2q2Z06mY8u&sig=ACfU3U13Fx54iNXWd3ltcgyTK1F2ZT32aw&hl=en&sa=X&ved=2ahUKEwjTq-zZwc77AhUpmWoFHZunC7sQ6AF6BQj1ARAD
DNase stock solution preparing for engineered heart tissues, 266 preparing for neuroendocrine/neuronal cell culture, 396 DNA standard solution, preparation ...
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66
Cell Biology: A Laboratory Handbook
https://books.google.com/books?id=02mLqzXwStUC&pg=PA441&lpg=PA441&dq=dnase+stock+solution+recipe&source=bl&ots=77d_gwmIIP&sig=ACfU3U3RyWGNs2RzJqyc0YfHwOEHg22zIQ&hl=en&sa=X&ved=2ahUKEwjTq-zZwc77AhUpmWoFHZunC7sQ6AF6BQj2ARAD
Preparation. of. High-Titer. Viral. Stocks. (Purified) ... DNase I solution: Dissolve 100mg bovine pancreatic deoxyribonuclease I (DNase I) in 10ml of 10mM ...
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https://books.google.com/books?id=02mLqzXwStUC&pg=PA441&lpg=PA441&dq=dnase+stock+solution+recipe&source=bl&ots=77d_gwmIIP&sig=ACfU3U3RyWGNs2RzJqyc0YfHwOEHg22zIQ&hl=en&sa=X&ved=2ahUKEwjTq-zZwc77AhUpmWoFHZunC7sQ6AF6BQj2ARAD
Preparation. of. High-Titer. Viral. Stocks. (Purified) ... DNase I solution: Dissolve 100mg bovine pancreatic deoxyribonuclease I (DNase I) in 10ml of 10mM ...
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