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1 A Gateway-Compatible Yeast One-Hybrid System - PMC - NCBI
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC528925/
We developed a Gateway-compatible yeast one-hybrid (Y1H) system that enables the rapid, large-scale identification of protein-DNA interactions using both ...
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2 Gateway-Compatible Yeast One-Hybrid Screens
http://cshprotocols.cshlp.org/content/2006/5/pdb.prot4590.full
Here we focus on genetic yeast “hybrid” assays that can be used in both small- and large-scale settings. Both yeast two-hybrid (Y2H) and yeast one-hybrid (Y1H) ...
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3 A High-throughput Gateway-Compatible Yeast One-Hybrid ...
https://experiments.springernature.com/articles/10.1007/978-1-61779-292-2_20
The yeast one-hybrid system is a genetic assay to identify direct binding of proteins to DNA elements of interest and is, therefore, instrumental in uncovering ...
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4 A High-throughput Gateway-Compatible Yeast One-Hybrid ...
https://link.springer.com/protocol/10.1007/978-1-61779-292-2_20
From: A High-throughput Gateway-Compatible Yeast One-Hybrid Screen to Detect Protein–DNA Interactions. Fig. 2. DNA bait cloning strategy.
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5 https://doi.org/10.1101/gr.2445504
https://doi.org/10.1101/gr.2445504
› ...
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6 A gateway-compatible yeast one-hybrid system. - Wikidata
https://www.wikidata.org/wiki/Q30841733
A gateway-compatible yeast one-hybrid system (English). 1 reference ... High-throughput yeast two-hybrid assays for large-scale protein interaction mapping.
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7 HETERODIMERIC TRANSCRIPTION FACTORS IN A YEAST ...
https://digital.wpi.edu/downloads/dj52w6176
The yeast one-hybrid (Y1H) system is an important and powerful assay for the ... expressing vectors in the yeast, a Gateway-compatible 2µ AD-fusion ...
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8 A yeast one‐hybrid and microfluidics‐based pipeline to map ...
https://www.embopress.org/doi/10.1038/msb.2013.38
We have generated a comprehensive, Gateway-compatible mouse (Mus musculus) ... The 745 TFs in Y1H-compatible AD-vectors are transformed into the yeast DNA ...
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9 ProQuest™ Two-Hybrid System with Gateway™ Technology
https://www.thermofisher.com/order/catalog/product/PQ1000101
The ProQuest Two-Hybrid System with Gateway Technology is an in vivo yeast-based system for identifying interactions between two proteins.
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10 Yeast one-hybrid assays for gene-centered human ... - Nature
https://www.nature.com/articles/nmeth.1764
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription ...
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11 Design of a single plasmid-based modified yeast one-hybrid ...
https://www.future-science.com/doi/10.2144/000112901
The yeast one-hybrid system (Y1H), a variant of the yeast two-hybrid system (Y2H) (2), is a powerful and commonly used in vivo genetic assay for ...
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12 Efficient Yeast One-/Two-Hybrid Screening Using a Library ...
https://academic.oup.com/pcp/article/51/12/2145/1944038
Yeast one-hybrid screening is widely used for the identification of transcription factors (TFs) that interact with specific DNA sequences.
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13 A High-Throughput Screening System for Arabidopsis ...
https://www.cell.com/molecular-plant/pdf/S1674-2052(14)60586-4.pdf
a Gateway-compatible Gal4-AD–TF library of 1589 Arabidopsis TFs, which can be easily screened by mating-based yeast-one-hybrid (Y1H) and ...
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14 Enhanced yeast one-hybrid assays for high-throughput gene ...
https://link.gale.com/apps/doc/A274228573/AONE?u=googlescholar&sid=googleScholar&xid=7dd16dfc
Interactions between transcription factors and regulatory genomic regions provide the first level of gene control. Gateway-compatible yeast one- ...
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15 Yeast one-hybrid assays: a historical and technical ...
https://www.semanticscholar.org/paper/Yeast-one-hybrid-assays%3A-a-historical-and-technical-Reece-Hoyes-Walhout/63d37ea430bb0747168d673f02948807b25bcf6b
226 Citations · Gateway-compatible yeast one-hybrid screens. · Gene-centered yeast one-hybrid assays. · Enhanced yeast one-hybrid (eY1H) assays for ...
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16 Application of the yeast one-hybrid technique to plant ...
https://www.tandfonline.com/doi/full/10.1080/13102818.2017.1378595
The yeast one-hybrid technique could help analyze DNA–protein interactions through the expression of reporter genes. It has become a frequently used ...
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17 C. elegans transcription factors - Horizon Discovery
https://horizondiscovery.com/-/media/Files/Horizon/resources/Technical-manuals/c-elegans-transcript-factors-manual.pdf
one-hybrid (Y1H) screens (Vermeissen et al., 2007). ... A Gateway-compatible yeast one-hybrid system. ... GATEWAY recombinational cloning: application to.
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18 Construction of Yeast One-hybrid Library and Screening of ...
https://www.researchsquare.com/article/rs-718598/v1.pdf
A high-throughput gateway-compatible yeast one-hybrid screen to detect protein-DNA interactions. Methods Mol Biol. 2012,. 786:335–355. 40.
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19 A Modified Yeast-one Hybrid System for Heteromeric Protein ...
https://typeset.io/papers/a-modified-yeast-one-hybrid-system-for-heteromeric-protein-2n7siak3ah
TL;DR: A Gateway-compatible yeast one-hybrid system that enables the rapid, large-scale identification of protein-DNA interactions using both small and ...
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20 A novel synthetic-genetic-array–based yeast one-hybrid ...
https://www.osti.gov/pages/servlets/purl/1625639
merate such TF–DNA interactions, for example, yeast one-hybrid. (Y1H) (Li and Herskowitz ... Gateway-compatible yeast one-hybrid screens.
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21 Generating Yeast One-Hybrid DNA-Bait Strains
http://www.molecularcloning.com/index.php?prt=242
Generating DNA-bait strains for Gateway-compatible Y1H screens involves three steps (Fig. 2A in the chapter introduction). The first is to generate an Entry ...
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22 Construction of gateway-compatible yeast two-hybrid vectors ...
https://www.ajol.info/index.php/ajb/article/view/78348/68712
that the newly constructed gateway-compatible two-hybrid vectors will be useful in analysis of protein ... Yeast two-hybrid is one of the.
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23 Isolation of plant transcription factors using a modified yeast ...
https://plantmethods.biomedcentral.com/articles/10.1186/1746-4811-2-3
Here we propose an adaptation of the yeast one-hybrid system for identification and cloning of transcription factors using a MATCHMAKER cDNA ...
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24 Two-hybrid screening - wikidoc
https://www.wikidoc.org/index.php/Two-hybrid_screening
The most common screening approach is the yeast two-hybrid assay. ... yeast two hybrid system · Gateway-Compatible Yeast One-Hybrid Screens.
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25 Discovering human transcription factor interactions ... - bioRxiv
https://www.biorxiv.org/content/10.1101/459305v2.full
Enhanced yeast one-hybrid (eY1H) assays provide an alternative ... of the firefly luciferase into a Gateway compatible vector generated from ...
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26 Analysis of the DNA-Binding Activities of the Arabidopsis ...
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0141044&type=printable
(yeast one-hybrid experiments) with a pool of selected ... open reading frames (ORFs) using the Gateway® technology with and without the ...
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27 catch of the day: a yeast one-hybrid assay identifies a novel dna
https://etd.ohiolink.edu/apexprod/rws_etd/send_file/send?accession=bgsu1339861904&disposition=inline
yeast one-hybrid assay was performed with the intent of identifying known and novel ... A gateway-compatible yeast one-hybrid system.
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28 Yeast One- and Two-Hybrid High-Throughput Screenings ...
https://oa.upm.es/53014/1/INVE_MEM_2017_284428.pdf
arrayed ORF libraries with DNA (Y1H) or protein (Y2H) baits not requiring robotics ... scaled up (i.e., robotization) and is compatible with several library.
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29 Two-hybrid screening - Wikipedia
https://en.wikipedia.org/wiki/Two-hybrid_screening
Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions ...
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30 A Modified Reverse One-Hybrid Screen Identifies ... - Frontiers
https://www.frontiersin.org/articles/10.3389/fpls.2016.00881/full
Here, we describe a yeast-based screening method that is able to ... cloning Kit (Invitrogen) and a Gateway compatible pGAD vector was ...
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31 A Novel NAC Transcription Factor, IDEF2, That Recognizes ...
https://www.jbc.org/article/S0021-9258(20)59795-0/pdf
*This work was supported by a grant-in-aid for scientific research on priority ... Construction of Reporter Vectors for the Yeast One-hybrid—.
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32 NIH Public Access - Gene-Quantification.info
https://gene-quantification.de/walhout-gene-centered-mapping-2011.pdf
high-throughput cloning of DNA baits, we have combined the Y1H system with Gateway cloning, a recombination-based method that is compatible with the ...
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33 from the Yeast Two-Hybrid System to the Mammalian Split ...
https://journals.asm.org/doi/10.1128/MMBR.05021-11
For example, some two-hybrid systems use only one hybrid protein (e.g., G protein fusion ... A gateway-compatible yeast one-hybrid system.
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34 Automated protein-dnA interaction screening of Drosophila ...
https://core.ac.uk/download/pdf/147978153.pdf
a high-throughput yeast one-hybrid (Y1H) system that enables ... ated 722 (96%) Gateway-compatible Entry clones (Invitrogen).
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35 Mapping methyl jasmonate-mediated transcriptional ... - PNAS
https://www.pnas.org/doi/pdf/10.1073/pnas.0711203105
yeast one-hybrid and transient transactivation assays identified ... Dupuy D, Vidal M, Walhout AJ (2004) A Gateway-compatible yeast.
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36 Yeast one-hybrid system—Matchmaker Gold - Takara Bio
https://www.takarabio.com/products/protein-research/two-hybrid-and-one-hybrid-systems/yeast-one-hybrid-system
Our Matchmaker Gold Yeast One-Hybrid Library Screening System provides a simple and efficient method for identifying and characterizing novel protein-DNA ...
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37 Protein Interaction (4) Yeast One-Hybrid Assay
https://www.creativebiomart.net/resource/principle-protocol-protein-interaction-4-yeast-one-hybrid-assay-374.htm
Yeast one-hybrid (Y1H) assay is an in vitro method to analyze the intracellular interaction between DNA and proteins. The general principles of Y1H rely on the ...
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38 An overview of the Yeast one-hybrid assay - Bitesize Bio
https://bitesizebio.com/25900/an-overview-of-the-yeast-one-hybrid-assay/
Y1H relies on the general principles of the yeast two-hybrid assay. In short, mammalian proteins are exogenously expressed in yeast and ...
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39 Yeast One-Hybrid Screening - Profacgen
https://www.profacgen.com/services/protein-analysis/nucleic-acid-protein-interactions/dna-protein-interactions/yeast-one-hybrid-screening
Yeast One-Hybrid is an effective method for cloning transcription factor genes (cDNAs) that specifically bind to target elements, based on the principle that ...
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40 takara taq polymerase protocol - Mastrosol
https://mastrosol.com.br/njqes2r/archive.php?page=takara-taq-polymerase-protocol
Deterministic and stochastic allele specific gene expression in single mouse blastomeres. ... and generated the plasmids for yeast three-hybrid analysis.
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41 Yeast One-hybrid (Y1H) Service - Creative Biolabs
https://www.creative-biolabs.com/yeast-one-hybrid-y1h-service.html
› yeast-one-hybrid-y...
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42 Murder suspect walked into bedroom shot man D C police say ...
https://askaila.online/68884-murder-suspect-walked-into-bedroom-shot-man-d-c-police-say-apprehended-caution-tape/
... to Monday-morning quarterback how one local government handled a local issue, ... How The Haunted Mansion Remains a Timeless Gateway to Horror Haunted ...
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43 Synthetic Biology and Metabolic Engineering in Plants and ...
https://books.google.com/books?id=9jwADAAAQBAJ&pg=PA290&lpg=PA290&dq=gateway+compatible+yeast+one+hybrid&source=bl&ots=adMJxWs1gZ&sig=ACfU3U0dJHTx1SOthCeuf63lKciJ1B-aUw&hl=en&sa=X&ved=2ahUKEwjX676a0tD7AhUUGlkFHZ7iD2kQ6AF6BQi9ARAD
A gateway-compatible yeast one-hybrid system. Genome Research, 14(10B), 2093–2101. Deplancke, B., Mukhopadhyay, A., Ao, W., Elewa, A. M., Grove, C. A., ...
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44 Yeast Gene Analysis - Page 128 - Google Books Result
https://books.google.com/books?id=nWfJc9swUFcC&pg=PA128&lpg=PA128&dq=gateway+compatible+yeast+one+hybrid&source=bl&ots=X4D5i4ZKEB&sig=ACfU3U0nHdDRUBXwrEvUmfDxChpYK8570w&hl=en&sa=X&ved=2ahUKEwjX676a0tD7AhUUGlkFHZ7iD2kQ6AF6BQjCARAD
A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a ... A gateway- compatible yeast one-hybrid system.
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45 Genomics and Molecular Genetics of Plant-Nematode Interactions
https://books.google.com/books?id=TpSIoRaZ4ngC&pg=PA177&lpg=PA177&dq=gateway+compatible+yeast+one+hybrid&source=bl&ots=LXgFtDnNhn&sig=ACfU3U1jGETCwP-VL5Vnn4FAlPT9Q2Vs5A&hl=en&sa=X&ved=2ahUKEwjX676a0tD7AhUUGlkFHZ7iD2kQ6AF6BQi2ARAD
The advance of a Gateway-compatible yeast one-hybrid system (Y1H) has enabled studies of differential gene expression controlled by specific C. elegans ...
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46 ' God is a Phillies fan' Philadelphia Catholic school cheers on ...
https://birseyyaz.com/65661-god-is-a-phillies-fan-philadelphia-catholic-school-cheers-on-the-home-team/
God of War: Ragnarök may be one of the most anticipated action-adventure sequels for PlayStation consoles, but fans don't know much about it, apart from the ...
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47 KSI blames Jake Paul for Liverpool s recent losses
https://birseykarala.com/13714-ksi-blames-jake-paul-for-liverpool-s-recent-losses/
1000% I accept, but one condition… You have to reply and admit that as of TODAY when it comes to boxing I am betr" Take a look at the exchange on ...
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48 Police Cargo Transport Truck APK Download for Android
https://arizakaydi.com/38852-police-cargo-transport-truck-apk-download-for-android/
... and Police Car transport truck simulator from one place to the other. ... Marion Cotillard tr s compatible avec son c l bre ex leurs signes astro ...
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49 Game Pass Hasn t Given Up on Final Fantasy 14 The Nerd ...
https://akpci.com/79708-game-pass-hasn-t-given-up-on-final-fantasy-14-the-nerd-stash
... s Playing 11 Player Stats Pitch Report for English Women s One Day Trophy 2022 Match 22 ... Compare Ford Explorer Hybrid vs Lincoln Aviator CarBuzz ...
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50 Identification of TCP13 as an Upstream Regulator of ATHB12 ...
https://www.mdpi.com/2073-4425/10/9/644/htm
In this study, we identify TCP13, a member of the TCP transcription factor family, as an upstream inhibitor of ATHB12. Yeast one-hybrid screening using a 1.2-kb ...
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