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1 Single-Molecule Studies of DNA Replisome Function - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020669/
Primosomes, a subcomplex of replisomes primarily composed of replicative helicases and primases, play an essential role in the progress of the ...
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2 Primosome - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/neuroscience/primosome
For example, the primosome is a replisome subassembly formed by the helicase, which unwinds DNA in the 5′- to 3′-direction, and the primase that synthesizes RNA ...
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3 The φX174‐type primosome promotes replisome assembly at ...
https://www.embopress.org/doi/10.1093/emboj/16.22.6886
It is distinguished from the oriC-type primosome by the involvement of host-encoded PriA, PriB, PriC and DnaT proteins in primosome assembly instead of the ...
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4 A Replisome's journey through the bacterial chromosome
https://www.frontiersin.org/articles/10.3389/fmicb.2015.00562/full
A new molecule of DNA polymerase is recruited to the replisome (step 2) and engages in the synthesis of a new Okazaki fragment. In this ...
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5 Replisome mechanics: lagging strand events that influence ...
https://academic.oup.com/nar/article/42/10/6497/2435022
The leading strand polymerase extends DNA in the same direction as helicase unwinding, while the lagging strand is copied in the opposite ...
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6 Review - The Eukaryotic Replisome Goes Under the Microscope
https://www.cell.com/current-biology/pdf/S0960-9822(16)30087-2.pdf
eukaryotic replisomes are also regulated in a wide variety of ... same positions that DNA binds to H3/H4 (Figure 5A; compare ... eukaryotic primosome.
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7 Understanding DNA replication by the bacteriophage T4 ...
https://www.jbc.org/article/S0021-9258(20)32964-1/abstract
We conclude with a brief comparison with other replisomes with emphasis on how coordinated ... The primosome (helicase gp41/primase gp61).
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8 Chapter 9: DNA Replication – Chemistry
https://wou.edu/chemistry/courses/online-chemistry-textbooks/ch450-and-ch451-biochemistry-defining-life-at-the-molecular-level/chapter-9-dna-replication-and-repair-2/
(a) The standard textbook model of a DNA Replisome showing the coupled and ... (b) Cartoon representations of complexes showing the differences in the ...
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9 Primosome and Replisome #neet #replication - YouTube
https://www.youtube.com/watch?v=CVGDvgApl28
BIOS BOTANY
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10 type primosome promotes replisome assembly at the ... - Wiley
https://nph.onlinelibrary.wiley.com/doi/pdf/10.1093/emboj/16.22.6886
The φX174-type primosome promotes replisome assembly at the site of recombination ... To distinguish between leading and lagging strand syn- ...
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11 replisome definition
https://groups.molbiosci.northwestern.edu/holmgren/Glossary/Definitions/Def-R/replisome.html
The DNA-replicating structure at the replication fork consisting of two DNA polymerase III enzymes and a primosome (primase and DNA helicase).
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12 Transcription leads to pervasive replisome instability in bacteria
https://elifesciences.org/articles/19848
Replication-transcription conflicts cause pervasive replisome instability. ... (For simplicity, we will ignore the distinction between focus ...
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13 Handoff from recombinase to replisome - PNAS
https://www.pnas.org/doi/10.1073/pnas.111007898
Primosome assembly proteins PriA, PriB, DnaT, and the DnaB–DnaC complex then ... Although no major differences in MuA crosslinking between STC1 and STC2 can ...
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14 Replication-Fork Dynamics - CSH Perspectives
https://cshperspectives.cshlp.org/content/6/1/a010157.full
1999). Handing off the primer to the polymerase seems to occur in a number of ways according to the different replisome architectures. In E.
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15 Replisome Dynamics during Chromosome Duplication
https://journals.asm.org/doi/abs/10.1128/ecosalplus.4.4.2
The leading strand polymerase continually synthesizes DNA in the direction of the replication fork, whereas the lagging-strand polymerase synthesizes short, ...
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16 25.1: DNA Replication - Biology LibreTexts
https://bio.libretexts.org/Bookshelves/Biochemistry/Fundamentals_of_Biochemistry_(LibreTexts)/03%3A_Unit_III-_Information_Pathway/25%3A_DNA_Metabolism/25.01%3A_DNA_Replication
In humans,, the primosome contains primase and DNA polymerase α (Polα), and makes RNA-DNA primers to which deoxynucleotides are added by ...
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17 Single-molecule live cell imaging of Rep reveals the dynamic ...
https://www.biorxiv.org/content/biorxiv/early/2018/09/29/430371.full.pdf
between an accessory replicative helicase and the replisome ... the fork, which failed to show a significant difference between wild type ...
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18 Replisome Assembly at oriC, the Replication Origin of E. coli ...
https://odonnell.rockefeller.edu/assets/pdfs/93.pdf
are likely explained by primase functioning with a mov- which two replisomes are assembled in opposite direc- ing helicase target. ... X174-type primosome.
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19 primosome complex Gene Ontology Term (GO:1990077)
http://www.informatics.jax.org/vocab/gene_ontology/GO:1990077
The primase synthesizes RNA primers that act as templates for complementary stand replication by the polymerase machinery. The primosome contains a number of ...
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20 The replisome guides nucleosome ... - Cell & Bioscience
https://cellandbioscience.biomedcentral.com/articles/10.1186/s13578-020-00398-z
Overview of eukaryotic replisome assembly. DNA replication starts at specific DNA elements termed replication origins. In the budding yeast ...
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21 (PDF) Understanding DNA replication by the bacteriophage ...
https://www.researchgate.net/publication/320186778_Understanding_DNA_replication_by_the_bacteriophage_T4_replisome
We conclude with a brief comparison with other replisomes with ... Assembly mechanism of the T4 lagging-strand primosome on forked DNA.
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22 Elaborated Action of the Human Primosome - MDPI
https://www.mdpi.com/2073-4425/8/2/62
The human primosome is a 340-kilodalton complex of primase (DNA-dependent RNA polymerase) and DNA polymerase α, which initiates genome replication by ...
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23 BIL 250 - Lecture 10a - This is bio.miami.edu
https://www.bio.miami.edu/dana/250/250S19_10a.html
The Replisome · Helicase breaks hydrogen bonds between the complementary DNA strands and unwinds the double helix. · Primase lays down an RNA primer on the DNA ...
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24 QuickGO::Term GO:0030894
https://www.ebi.ac.uk/QuickGO/term/GO:0030894
› QuickGO › term
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25 DNA replication at the single-molecule level
https://pubs.rsc.org/en/content/getauthorversionpdf/C3CS60391A
guide the reader through the different aspects of important single- ... (C) T4 primosome–replisome reconstitution in a magnetic trap.
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26 Replisome-Mediated DNA Replication - Annual Reviews
https://www.annualreviews.org/doi/abs/10.1146/annurev.biochem.70.1.181
Replisome-Mediated DNA Replication. Annual Review of Biochemistry ... (a) Comparison of programmable sequence-specific genome editing nucleases.
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27 Solved 7. (a) Give one difference between replisome and
https://www.chegg.com/homework-help/questions-and-answers/7-give-one-difference-replisome-primosome-1-b-translation-unidirectional-dna-replication-b-q84437989
Transcribed image text: 7. (a) Give one difference between replisome and primosome. (1) (b) Why is translation unidirectional while DNA replication is a ...
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28 Escherichia coli K-12 substr. MG1655 primosome - BioCyc
https://biocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=CPLX0-3922
Summary: The primosome is a six-protein complex that is involved in restart of stalled replication forks, as well as in replication initiation in certain phages ...
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29 Examination of the roles of a conserved motif in the PriA ...
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0255409
The PriA DNA helicase is a central component of the primosome and functions to recognize and remodel abandoned replication forks for replisome ...
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30 Handoff from Recombinase to Replisome - JSTOR
https://www.jstor.org/stable/3056161
In the transition to DNA synthesis, the molecular chaperone CIpX acts first to weaken the transpososome's ... Primosome assembly proteins PriA, co.
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31 Mechanisms of DNA Replication Restart - Keck Lab
https://kecklab.bmolchem.wisc.edu/mechanisms-of-dna-replication-restart/
“DNA replication restart” is mediated several proteins (PriA, PriB, PriC, and DnaT in E. coli) that function in a coordinated manner to reload the replisome ...
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32 Protein-Protein Interactions in the Bacterial Replisome
https://www.asbmb.org.au/oldsite/magazine/2004-April_Issue35-1/Showcase%202%20-%20Schaeffer%20et%20al.%20SMALLER.pdf
Protein-Protein Interactions in the Bacterial Replisome ... primosome (now renamed the replication restart primosome) in several mechanisms ...
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33 Basic Mechanisms of Replication
http://www.bx.psu.edu/~ross/workmg/Replication1Ch5.htm
We will point out parallels and homologies between bacterial and eukaryotic ... the replication machinery in a large replisome (polymerase and primosome) is ...
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34 Dynamics of the bacterial replisome: Biochemical and single ...
https://ro.uow.edu.au/cgi/viewcontent.cgi?article=1509&context=theses1
between the different enzyme activities of the replisome (Hamdan and Richardson, 2009; Shereda et al.,. 2008). Figure P.1: The Escherichia coli replisome.
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35 Transcription leads to pervasive replisome instability in bacteria
https://go.gale.com/ps/i.do?id=GALE%7CA481045116&sid=googleScholar&v=2.1&it=r&linkaccess=abs&issn=2050084X&p=HRCA&sw=w
Although the replisome, the cellular machinery responsible for DNA ... (For simplicity, we will ignore the distinction between focus loss and fork ...
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36 Classifying the Functionality of Primosome Protein A in ...
https://ecommons.udayton.edu/cgi/viewcontent.cgi?article=1002&context=uhp_theses
unlike the homologous recombination pathway, origin-independent replisome reloading remains largely undefined in the bacterium. Upon direct comparison with ...
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37 Reconstitution of the B. subtilis Replisome with 13 Proteins ...
https://www.colorado.edu/lab/mchenry/2010-23702-mc-37-273
a different set of proteins that intervene between the PriA initiation ... anistic-based probing of important differences, including the.
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38 Mechanism of Concerted RNA-DNA Primer Synthesis by the ...
https://www.osti.gov/pages/biblio/1769433
The human primosome, a 340-kilodalton complex of primase and DNA ... the CMG helicase to DNA polymerase α in the eukaryotic replisome
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39 2PNH: Escherichia coli PriB E39A variant - RCSB PDB
https://www.rcsb.org/structure/2PNH
In E. coli, the PriA-dependent pathway is the major replication restart mechanism and requires primosome proteins PriA, PriB, and DnaT for replisome ...
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40 RecA and RecB: probing complexes of DNA repair proteins ...
https://arxiv.org/pdf/2110.01573
action of replication-reloading primosome proteins help in the establishment of an intact replisome to resume replication (16). Recombination proteins ...
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41 What is a primosome, and what are its functions ... - Numerade
https://www.numerade.com/questions/what-is-a-primosome-and-what-are-its-functions-what-essential-enzymes-are-present-in-the-primosome-w/
What essential enzymes are present in the primosome? What are the major components of the E. coli replisome? How can geneticists determine whether these ...
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42 Genetics Ch. 11 Flashcards - Quizlet
https://quizlet.com/318528082/genetics-ch-11-flash-cards/
What is a key difference between DNA polIII and DNA ligase? ... What is the advantage of a primosome and a replisome as opposed to having all replication
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43 Structural Insight into the DNA-Binding Mode of the ... - Hindawi
https://www.hindawi.com/journals/bmri/2014/195162/
Replication restart primosome is a complex dynamic system that is essential for bacterial survival. This system uses various proteins to reinitiate chromosomal ...
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44 The replisome guides nucleosome assembly during DNA ...
https://d-nb.info/1211477819/34
Six OB-folds in the different sub- units contribute to the ssDNA-binding ability of RPA. Recently, we found that, in budding yeast, the previously.
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45 Structure of a helicase&ndash - Nature
https://www.nature.com/articles/ncomms3495.pdf?origin=ppub
During the assembly of the bacterial loader-dependent primosome, ... is a structural difference between the NTD of BsuDnaI and the.
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46 Enzymes of DNA replication - SlideShare
https://www.slideshare.net/magendiramanivinayag/enzymes-of-dna-replication
About 400 Pol I molecules exist in a single bacterium. DNA polymerase I only makes an average of 20 phosphodiester bonds before dissociating from the template.
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47 DNA Synthesis - California State University Dominguez Hills
http://www2.csudh.edu/nsturm/CHEMXL153/DNASynthesis.htm
(b) In the second mechanism replication of both strands is initiated at one ... (dNTP's) are required for DNA synthesis (note the only difference between ...
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48 5
http://mol-biol4masters.masters.grkraj.org/html/Prokaryotic_DNA_Replication8-Primosomes.htm
Primosomes are complex of proteins that produce RNA primer at the time of DNA replication. Enzymatic analyses of primosome assembly at chromosomal and plasmid ...
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49 Duplex opening by primosome protein PriA for replisome ...
https://www.uniprot.org/citations/10356325
The helicase activity of PriA could then open the duplex to create the DnaB binding site. In a tightly coupled reaction on synthetic forked ...
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50 DNA replication of prokaryotes - Page 2
https://reasonandscience.catsboard.com/t1849p15-dna-replication-of-prokaryotes
(B) Conformational differences between DnaB hexamers bound to either DnaC ... primosome associates with two DNA polymerase enzymes to form a replisome.
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51 DNA Replication in Bacteria (E. coli) Script
http://aris.gusc.lv/ChemFiles/VoetPrattBiochemistry99/Lessons/24-2b_ReplicateDNA/DNA_RepScript.htm
... which together with the primosome, form an assembly named the "replisome". ... All DNA polymerases synthesize DNA in a 5' to 3' direction, that is, ...
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52 Molecular mechanism of DNA replication - Khan Academy
https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/replication/a/molecular-mechanism-of-dna-replication
One new strand, which runs 5' to 3' towards the replication fork, is the easy one. This strand is made continuously, because the DNA polymerase is moving in the ...
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53 Replisome-mediated DNA replication - ProQuest
http://search.proquest.com/openview/e9aeca8894ae2b4dffb487f0dd1c00b1/1?pq-origsite=gscholar&cbl=190
An obvious difference is that ATP hydrolysis by the clamp loader is not required for formation of the Beta clamp . gamma complex . DNA complex; ATP(gamma)S is ...
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54 Duplex opening by primosome protein PriA for ... - EPA - HERO
https://hero.epa.gov/hero/index.cfm/reference/details/reference_id/7046458
PriA and other primosome assembly proteins of Escherichia coli recruit the major replicative helicase DnaB for replisome assembly during ...
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55 Biological Physics: Poincaré Seminar 2009
https://books.google.com/books?id=TSvm9a97vLUC&pg=PA118&lpg=PA118&dq=difference+between+primosome+and+replisome&source=bl&ots=Xh5ZrK4oZ-&sig=ACfU3U1SyzN1Odk7aefh0zwEDmBupBGW6A&hl=en&sa=X&ved=2ahUKEwiMrvyk2tP7AhVKlIkEHVt_DO0Q6AF6BQj1ARAD
Our approach does not allow us to directly visualize the different proteins of the replisome but only to measure their overall activity as a change in the ...
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56 Principles of Genetics - Page 39 - Google Books Result
https://books.google.com/books?id=NBB0CgAAQBAJ&pg=RA2-PA39&lpg=RA2-PA39&dq=difference+between+primosome+and+replisome&source=bl&ots=g-NXsZ29iP&sig=ACfU3U2R4sETKkcabKvdZVKO15tAurY3Pw&hl=en&sa=X&ved=2ahUKEwiMrvyk2tP7AhVKlIkEHVt_DO0Q6AF6BQj3ARAD
10.26 What is a primosome, and what are its functions? ... enzymes are present in the primosome? What are the major components of the E. coli replisome?
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57 The Bacterial Cell: Coupling between Growth, Nucleoid ...
https://books.google.com/books?id=zEhADgAAQBAJ&pg=PA57&lpg=PA57&dq=difference+between+primosome+and+replisome&source=bl&ots=0eKO-NO91g&sig=ACfU3U2C7AktVX4e9y0Zo6HIx-eZW3OmfA&hl=en&sa=X&ved=2ahUKEwiMrvyk2tP7AhVKlIkEHVt_DO0Q6AF6BQjfARAD
However, the B. subtilis replisome also exhibits some notable differences ... the helicase and DnaG, forming a “primosome” complex (Rannou et al., 2013).
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58 Replisome - Wikiwand
https://www.wikiwand.com/en/Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands.
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59 T4 phage replisome - Penn State - Elsevier
https://pennstate.pure.elsevier.com/en/publications/t4-phage-replisome
These proteins form sub-complexes including DNA polymerase holoenzyme (gp43, gp45, and gp44/62), primosome (gp61, gp41, and gp59), and single- ...
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