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1 Black Hole Quencher (BHQ) dyes
https://www.biosearchtech.com/support/education/fluorophores-and-quenchers/black-hole-quencher-dyes
Black Hole Quencher™ dyes are true "dark" quenchers with no fluorescent signal, improving signal to noise ratios. Through a combination of FRET and static ...
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2 Characterization of Dark Quencher Chromophores as ... - NCBI
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368131/
Dark quenchers are chromophores that primarily relax from the excited state to the ground state nonradiatively (i.e., are dark). As a result, they can serve ...
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3 qPCR Probes—selecting the best reporter dye and quencher
https://www.idtdna.com/pages/education/decoded/article/qpcr-probes-selecting-the-best-reporter-dye-and-quencher
IDT product details for Dark Quenchers modifications. ... Dabcyl is a relatively weak dark quencher and is best suited for use in Molecular Beacons where ...
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4 Dark quencher - chemeurope.com
https://www.chemeurope.com/en/encyclopedia/Dark_quencher.html
Dark quencher A quencher is a non-fluorescent dye that absorbs light but ... and quencher at the base of the stem is used: a unlabelled SNP specific PCR ...
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5 Dark Quencher Modifications - Eurofins Genomics
https://eurofinsgenomics.eu/en/dna-rna-oligonucleotides/custom-dna-rna-oligos/custom-dna-oligos/dna-modifications/dark-quencher/
Improve the sensitivity of your quantitative real-time PCR by the use of non-fluorescent dark quenchers.
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6 Black Hole Quencher® - Sigma-Aldrich
https://www.sigmaaldrich.com/JP/ja/technical-documents/technical-article/genomics/qpcr/black-hole-quenchers
Two commonly used quenchers, TAMRA™ and DABYCL, limit the ultimate sensitivity and flexibility of qPCR. TAMRA is not a dark quencher and therefore ...
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7 Comparison of TaqMan™ and Epoch Dark Quenchers ...
https://www.sciencedirect.com/science/article/pii/S0890850804000155
... of TaqMan™ and Epoch Dark Quenchers™ during real-time reverse transcription PCR ... The Epoch Dark Quencher™ fluorochrome consists of a non-fluorescent ...
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8 qFlamma® Quenchers - BioActs Official Website
https://www.bioacts.com/item/item_cates2.php?code=105
Dark quenchers are substances that absorb excitation energy from a fluorophore and ... in protease activity assays, nucleic acid hybridization, PCR, etc.
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9 Dark Quencher Modifications - Eurofins India
https://www.eurofins.in/genomics/oligos/custom-dna-oligos/dark-quencher-modifications/
Dark quencher extends quenching efficiency. Improve the sensitivity of your quantitative real-time PCR by using one of these quenchers: Dabcyl [DAB]:.
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10 Quencher - biomers.net Oligonucleotides
https://www.biomers.net/en/products/dna/dyes/quencher.html
Increasingly, therefore non-fluorescent quencher, so-called Dark Quenchers, have established. In contrast, Dark Quenchers are molecules that can absorb energy ...
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11 qFlamma® Black01 Biotin - Diagnocine
https://diagnocine.com/Product/qFlamma-Black01-Biotin/61817
We offer qFlamma® Black01 Biotin as suitable dark quencher for designing a variety of activatable probes for affinity chromatography, PCR, in situ nucleic ...
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12 Which Quencher Molecule is Best for TaqMan® Probes in ...
https://www.youtube.com/watch?v=yPV30ChD3hk
Thermo Fisher Scientific
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13 Is it crucial for TaqMan RT-PCR assay to specify the type of ...
https://www.researchgate.net/post/Is-it-crucial-for-TaqMan-RT-PCR-assay-to-specify-the-type-of-quencher-in-reaction-setup
As far as I know, "Non-fluorescent quencher (NFQ)" or "dark quencher" is the term used for all quenchers that don't emit a signal of their own. A black hole ...
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14 eurogentec-RT-PCR-booklet.pdf - Gene-Quantification.info
https://www.gene-quantification.de/eurogentec-RT-PCR-booklet.pdf
A dark quencher gives a higher signal to noise ratio, thus more sensitive assay; that is why we do recommend to use dark quenchers when doing multiplexing assay ...
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15 Access Dyes & Quenchers – IP Friendly Solution - Eurogentec
https://www.eurogentec.com/en/access-dyes-and-quenchers
Access™ Dyes and Quenchers include FAM, Yakima Yellow®, ROX, HiLyte Fluor™, BHQ®, MGB-EDQ, QXL® and other fluorescent dyes and dark quenchers on request.
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16 Product fluorescent-dyes-black-hole-quencher
http://www.ruixibiotech.com/pt/black-hole-quencher%20/page1
› page1
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17 Quencher labels for fluorescent hybridization probes
http://www.molecular-beacons.org/toto/Marras_quenchers.html
Quencher. Absorption Maximum (nm). Deep Dark Quencher I A. 430. Dabcyl. 475. Eclipse B. 530. Iowa Black FQ C. 532. Black Hole Quencher 1 D.
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18 Detecting Signal in qPCR: From DNA Binding Dyes to BHQ ...
https://bitesizebio.com/23955/detecting-signal-in-qpcr-from-dna-binding-dyes-to-bhq-probes/
In the early 1990s, quantitative (q) PCR was in its infancy, and despite PCR ... BHQ dyes are a type of dark quencher that, like DABCYL, ...
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19 Fluorophores and Quenchers - BioCat GmbH
https://www1.biocat.com/categories/pcr-and-qpcr/qpcr-related-products/fluorophores-and-quenchers
Biosearch Technologies Dyes for Real Time PCR ... Coupled with Biosearch's Black Hole Quencher (BHQ) dark quenchers you now can utilize the full potential ...
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20 Universal probe library probes.... - Real-Time PCR
http://www.protocol-online.org/biology-forums/posts/29124.html
I have recently started doing some real time PCR and was suggested to ... with fluorescein (FAM) and 3'- proximal with a dark quencher dye.
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21 Nitrodiarylethenes as fluorescence quenchers for nucleic acid ...
https://patents.google.com/patent/WO2018162986A2/en
The oligonucleotide-quencher conjugates may be used in detection methods for nucleic ... azo dye-based quenchers are Eclipse Dark Quencher (U.S. Patent No.
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22 Dark quencher - Wikiwand
https://www.wikiwand.com/en/Dark_quencher
A dark quencher is a substance that absorbs excitation energy from a ... An unlabeled SNP specific PCR primer (one of many) with a specific 5' tail binds to ...
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23 Efficient Synthesis of a Wide‐Range Absorbing ...
https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/chem.201801319
... for dark quenchers of fluorescence in DNA hybridization probes. ... to also be useful in a practical PCR assay for the quantification of ...
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24 Fluorescent Probes Manual - Jena Bioscience
https://www.jenabioscience.com/images/dded44ba94/Fluorescent_Probes_Manual.pdf
Both the dual labeled fluorescent probe and the PCR ... Black Hole dark quencher (BHQ-1) probes are an advanced.
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25 Unique Real-Time PCR technologies - ELITechGroup
https://www.elitechgroup.com/product/elitechgroup-real-time-pcr-proprietary-technologies
Pleiades probes are proprietary probes concentrating all ELITechGroup PCR technologies, designed with a Minor Groove Binder (MGB), Eclipse® Dark Quencher, ...
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26 Two-dye and one- or two-quencher DNA probes for real ... - Gale
https://go.gale.com/ps/i.do?id=GALE%7CA394089315&sid=googleScholar&v=2.1&it=r&linkaccess=abs&issn=16182642&p=AONE&sw=w
Two-dye and one- or two-quencher DNA probes for real-time PCR assay: synthesis and ... The reaction mixtures were left overnight in the dark, centrifuged, ...
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27 Eclipse - metabion
https://www.metabion.com/learning-platform/modifications/quenchers/eclipse/
As dark quenchers, Eclipse® absorbs energy without emitting fluorescence. It is in fact a non-fluorescent chromophore. Quencher eclipse. Metabion offers Eclipse ...
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28 Dyes & Quenchers - 上海兆维科技发展有限公司
https://www.hongene.com/cn/classify-91.html
... including microscopy and PCR. These phosphoramidites may be used on all automated DNA synthesizers using standard synthesis procedures. A dark quencher ...
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29 TaqMan™ QSY Probe - Thermo Fisher Scientific
https://www.thermofisher.com/order/catalog/product/4482777
Related applications: Real Time PCR (qPCR) ... Current users of dark quencher probes, such as Black Hole Quencher (BHQ™) probes, can design custom QSY ...
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30 Real-time PCR as a Diagnostic Tool for Bacterial Diseases
https://www.medscape.com/viewarticle/774873_2
A TaqMan probe carries both a fluorophore and a dark quencher (which absorbs excitation energy from the fluorescent dye) at its extremities; hydrolysis of ...
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31 Probe Compatibility and Multiplexing with Luna® Universal ...
https://www.neb.com/-/media/nebus/files/application-notes/appnote_probe-based_qpcr.pdf?rev=fb711076352c4136a9898dd1959f8a10
Quantitative PCR (qPCR) is a commonly used molecular biology technique that ... dark quencher (e.g. Black Hole Quencher®, LGC. Biosearch; Iowa Black®, IDT; ...
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32 Quencher - Microsynth - CH
https://www.microsynth.com/quencher.html
Dabcyl is a relatively weak dark quencher and is best suited for use in molecular beacon probes where the hairpin places the quencher and fluorescent dye in ...
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33 Fluorescent Probe - The Shining Star of PCR - Kilobaser
https://kilobaser.com/fluorescent-probes-the-shining-star-of-pcr/
COME TO THE DARK SIDE. Further improvement for fluorescent probes came in 2000 with the introduction of the first non-fluorescent quencher, ...
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34 IRDye QC-1 Dark Quencher - The Next Step in Near Infrared ...
https://www.yumpu.com/en/document/view/8990677/irdye-qc-1-dark-quencher-the-next-step-in-near-infrared-
PCR. IRDye QC-1 is also a dark quencher. To demonstrate. this property, the intrinsic fluorescence spectrum of.
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35 BHQ-2 Phosphoramidite - Oligo Synthesis - Cambio
https://cambio.co.uk/0/709/34/products/5'-bhq-2-phosphoramidite/
BHQ dyes are robust dark quenchers that very nicely complement our existing ... The dark quencher most typically used in a Molecular Beacon is Dabcyl.
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36 Black Hole Quencher 3 -BHQ3 Oligonucleotide Modification
https://www.biosyn.com/oligonucleotideproduct/black-hole-quencher-3-bhq3-oligonucleotide-modification.aspx
BHQ®-3 labeled oligonucleotides are used in many applications, including dual-labeled fluorogenic probes for real-time PCR. This dark quencher has strong ...
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37 Black Hole Quencher Technical Brochure Now Available
https://www.bioprocessonline.com/doc/black-hole-quencher-technical-brochure-now-av-0001
NOVATO, CA (March 21, 2001) - A technical brochure describing an exciting new class of high-efficiency dark quenchers, the Black Hole Quencher dyes from ...
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38 Enzyme reagent kit - Yakima Yellow®-Eclipse® Dark Quencher
https://www.medicalexpo.com/prod/eurogentec/product-299772-1053034.html
Universal Exogenous qPCR positive Control for Qualitative validation (IPC & SPC): Real-Time PCR assays are prone to inhibition by various substances found in ...
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39 3'-BHQ-2 - e-oligos Modifications
http://www.e-oligos.com/eoweb/products/mod_detail.asp?modid=126
Black Hole Quencher-2 (BHQ-2) is classified as a dark quencher (a non-fluorescent ... fluorescent DNA probe purity versus performance in real-time PCR.
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40 Recommended dye combinations for multiplex qPCR
https://www.science.smith.edu/cmbs/wp-content/uploads/sites/36/2018/11/Recommended-dye-combinations-for-multiplex-qPCR.pdf
Highly ef cient dark quenchers, especially those used in combination with a secondary quencher such as the ZEN™ Quencher (/pages/products/qpcr-and-pcr/gene-.
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41 primetime-custom-qpcr-probes-flyer.pdf - NET
https://sfvideo.blob.core.windows.net/sitefinity/docs/default-source/flyer/primetime-custom-qpcr-probes-flyer.pdf?sfvrsn=b4ab3707_16
digital PCR. PrimeTime qPCR Probes are available in a wide variety of dye-quencher combinations (Table 1) that are compatible with common qPCR instruments.
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42 3'-EBQ570 CPG (1000Å) - BIONEER
https://eng.bioneer.com/20-m-3025-1-cfg.html
EBQ(Excellent Bioneer Quencher) is a new dark quencher material developed by Bioneer Corporation.※ This product is shipped in dry ice.
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43 Time Gating Improves Sensitivity in Energy Transfer Assays ...
https://pubs.acs.org/doi/10.1021/ja0452368
... commercial assays such as real-time polymerase chain reaction (PCR) use ... It is shown that because the terbium to dark quencher energy ...
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44 Quenchers - Creative Biolabs
https://www.creative-biolabs.com/gene-therapy/quenchers.htm
TAMRA-modified oligonucleotides have played an important role in both FRET and real-time PCR applications. Creative Biolabs offers many different dark and ...
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45 Guidance document on multiplex real-time PCR methods
https://gmo-crl.jrc.ec.europa.eu/doc/KJNA30708ENN.en.pdf
When choosing quenchers for a multiplex assay it is highly recommended to use non- fluorescent quenchers also called dark quenchers. These quenchers absorb ...
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46 QuantiNova® LNA® Probe PCR Handbook - QIAGEN
https://www.qiagen.com/ie/resources/download.aspx?id=55c7e4d6-8ad6-43cb-8eb2-3082b42ca579&lang=en
Plate layout for QuantiNova LNA Probe PCR Focus panels . ... QuantiNova LNA Probe PCR 96-well and 384-well panels . ... collection (FAM-Dark Quencher).
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47 DNA Oligonucleotides Synthesis Service - BRIC
https://www.ibric.org/myboard/down.php?Board=rnd_test&filename=Brochure_SFC%20DNA%20probe%20Service_2018.pdf&id=394&fidx=1
SFCQ1 is a newly-developed dark quencher ... SFCQ1 Quencher for qPCR probes ... primers (0.5uM, respectively), and dual-labeled probes (0.2uM). PCR data.
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48 Real-Time PCR/Quantitative PCR (qPCR) – An Introduction
https://old.abmgood.com/marketing/knowledge_base/polymerase_chain_reaction_qpcr.php
Therefore, quencher dyes in TaqMan probes are usually dark quenchers that do not emit their own fluorescence (31)(14). In non-FRET static quenching, the ...
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49 HPA real-time PCR - inno-train
https://www.inno-train.de/en/products/hpa-typing/hpa-real-time-pcr/
PCR-SSP method without gel electrophoresis within 90 minutes ... “EclipseTM Dark Quencher”, “EclipseTM Quencher” are trademarks of ELITechGroup, Inc., ...
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50 INTRODUCTION PRODUCTS FOR SIRNA RESEARCH
https://www.glenresearch.com/media/contentmanager/content/glenreport/GR17-1.pdf
fluorescence arising from a dark quencher, ... fluorescent, dark quencher probes are not ... to incorporate a fluorophore by PCR, nick.
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51 What are the fluorescent probes that can be used for the device?
https://www.pcr-nsg.jp/en/faq/895
... using FAM (a fluorescent probe excited|A mobile real-time PCR system, PicoGene™, ... Be sure to select a dark quencher to absorb excitation energy.
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52 Simultaneous Absolute Quantification of Target and Control ...
https://academic.oup.com/clinchem/article-abstract/47/3/486/5639255
... transcription-PCR (RT-PCR) as a quantitative analytical tool, ... Using 4-(4′-Dimethylaminophenylazo)benzoic Acid as a Dark Quencher Dye.
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53 Introduction to PCR Primer & Probe Chemistries | Bio-Rad
https://www.bio-rad.com/en-us/applications-technologies/introduction-pcr-primer-probe-chemistries?ID=LUSOJW3Q3
Commonly used fluorescent reporter-quencher pairs are fluorescein (FAM), which emits green fluorescence, and Black Hole Quencher 1 dye. Real-time PCR product ...
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54 Stabilized Nucleic Acid Dark Quencher-Fluorophore Probes
https://patentimages.storage.googleapis.com/40/a8/c1/2fa900bba18ccc/EP2757091A1.pdf
The fluorogenic probe consists of an oligonucleotide labeled with both a fluorescent reporter dye and a quencher dye. During PCR, this probe ...
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55 GMO Screening Kit (35S, NOS, bar, FMV) Quick Reference ...
https://www.bc-diagnostics.com/downloads/products/qrp/R_302_17_21_GMO%20Screening.pdf
The above mentioned real-time PCR instruments are registered trademarks of their ... This kit contains probes with a non-fluorescent „dark“ quencher and no ...
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56 How does dark quencher work? Explained by FAQ Blog
https://faq-blog.com/how-does-dark-quencher-work
Expert Answers: A dark quencher (also known as a dark sucker) is a ... TaqMan PCR is a type of real-time PCR and uses a nucleic acid probe ...
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57 3' Eclipse® Dark Quencher 200 nmol - Eurogentec - Labscoop
https://www.labscoop.com/p/Eurogentec-3-Eclipse-Dark-Quencher-200-nmol/3883871
3' Eclipse® Dark Quencher 200 nmol: MD-QU300-03020 by Eurogentec at Labscoop.com - Read reviews, citations, datasheets, protocols & more.
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58 qPCR Sets New Standards for Gene-Expression Analysis
https://www.genengnews.com/magazine/142/qpcr-sets-new-standards-for-gene-expression-analysis/
Quantitative real-time polymerase chain reaction (qPCR) continues to ... double-quenched probe design that employs a dark quencher (ZEN™) ...
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59 BHQ® Patent Issued to Biosearch Technologies
https://www.technologynetworks.com/genomics/news/bhq-patent-issued-to-biosearch-technologies-188029
The patent detailing this technology is titled "Dark quenchers for ... aquaculture pathogen testing and the world's first PCR-based test for ...
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60 Characterization of Dark Quencher Chromophores as ... - CORE
https://core.ac.uk/download/pdf/81959034.pdf
ABSTRACT Dark quenchers are chromophores that primarily relax from the excited state to the ... ployed in real-time PCR (11) and genotyping as molecular.
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61 Premade clone - E-nnovation Life Sciences
https://ennovationlifesciences.com/wp-content/uploads/2016/06/Cateloge_18-06-16.pdf
Enhanced and optimised Taq PCR mastermix for ... In contrast, the BHQ dyes are highly efficient dark quenchers ... other dark quencher dyes, such as DABCYL.
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62 All about Probe-Based Real-Time qPCR Assays | GoldBio
https://goldbio.com/articles/article/all-about-probe-based-qPCR
In molecular biology, the real-time quantitative PCR (qPCR) method is a favorite ... BHQ™ dyes are dark quenchers and they re-emit their energy as heat, ...
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63 Real-time PCR II: probe methods | Medical Laboratory Observer
https://www.mlo-online.com/home/article/13005445/realtime-pcr-ii-probe-methods
A second fluorescence event then occurs, and the quencher ... process is the availability of “dark quenchers,” or quencher dyes which ...
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64 SOP/CISA/ASF/PCR/3
https://www.asf-referencelab.info/asf/images/ficherosasf/SOP%202018/SOP-ASF-PCR-3-2018.pdf
Molecular diagnosis of African swine fever (ASF) by a new real-time PCR ... residues in length, labeled with FAM dye at the 5' and a dark quencher dye at.
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65 qPCR Workshop held at NFSTC - STRBase
https://strbase.nist.gov/pub_pres/qPCR_Orrego4.pdf
1993 – First real-time PCR detection experiments to show utility for DNA ... better quenching of reporter by quencher (“dark” is darker, so less.
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66 Introduction to Quantitative PCR - Agilent
https://www.agilent.com/cs/library/brochures/Brochure_Guide%20to%20QPCR_IN70200C.pdf
guidelines on the choice of dark quenchers are given in the Dye and Quencher Choice section of this guide. When designing primers and probes for multiplex ...
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67 Double Labeled Probe - Nihon Gene Research Laboratories
http://www.ngrl-japan.com/synthesize/double_labeled_probe.html
We line up hydrolysis probes of which both ends are labeled for PCR. ... Dark quencher does not emit fluorescent light by itself.
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68 Evaluation of TaqMan qPCR System Integrating Two ... - Nature
https://www.nature.com/articles/srep41392
Real-time PCR utilising 5′ nuclease or hydrolysis probes, also known as TaqMan qPCR ... NFQ non fluorescent quencher, DQD dark quencher dye.
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69 Part:BBa K1319001 - iGEM Parts
http://parts.igem.org/Part:BBa_K1319001
This part is a RFC[25] dark quencher that is based upon K1319000 ... The PCR clearly shows that the used plasmid had the correct insert.
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70 Detection of fluorescent-labeled probes at sub
https://opg.optica.org/vjbo/viewmedia.cfm?uri=oe-16-23-19253&seq=0
A dark quencher is connected at the 3' prime. When detection is made, the discriminating probe attaches to the target DNA. Similar to real time PCR reaction ...
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71 Optimizing primer–probe design for fluorescent PCR
https://www.semanticscholar.org/paper/Optimizing-primer%E2%80%93probe-design-for-fluorescent-PCR-Proudnikov-Yuferov/ebd9b4b0564dcbee1603c91f4390e2be46f9f827/figure/1
Two-dye and one- or two-quencher DNA probes for real-time PCR assay: ... be used as dark quencher fluorophore in real-time fluorescence PCR.
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72 AzaPc quencher small molecule (tool compound) - Ximbio
https://ximbio.com/reagent/160663/azapc-quencher-small-molecule-tool-compound
... for dark quenchers of fluorescence in DNA hybridization probes. ... The AzaPc-labeled probe proved to also be useful in a practical PCR assay for the ...
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73 Black Hole Quencher Dyes
http://www.bioluminor.com/static/upload/file/20211019/1634606932537958.pdf
Black Hole Quencher Dyes. -The Inescapable Solution for DNA Hybridization Probes. A NEW class of high efficiency. Dark quenchers. Custom-synthesized.
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74 FLUORESCENT DYES, PROBES & QUENCHERS
https://fluoroprobes.com/wp-content/uploads/2020/09/catalogue-fluoroprobes-2021-web.pdf
Universal Black 3 Quencher (UBQ-3, Black Hole Quencher®-3 (BHQ®-3) equivalent) is a dark, far- red nonfluorescent acceptor dye with no native emission. UBQ-3 is ...
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75 ViennaLab RealFast™ Assays - Oasis Diagnostics®
https://4saliva.com/viennalab/viennalab-realfast-assays/
The ViennaLab RealFast™ Assays are based on real-time PCR and hydrolysis ... a fluorescent reporter dye at the 5´-end and a quencher dye at the 3´-end.
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76 Probe Systems - Taqman - Some Basic Principles
https://passel2.unl.edu/view/lesson/c380e2cd33cd/10
When the quencher is located near the reporter, it prevents the reporter from fluorescing. This is the state of the probe in the beginning of the PCR ...
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77 2013 - Bio-Resource
http://technologyinscience.blogspot.com/2013/
Real Time PCR (qPCR) - Fluorescent Primer Probe Based Chemistries ... containing a fluorescent reporter dye at the 5' end and quencher at ...
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78 What is high-fidelity PCR? | By Applied Biosystems - Facebook
https://www.facebook.com/appliedbiosystems/videos/what-is-high-fidelity-pcr/10157923188645117/
Applied Biosystems
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79 Molecular Microbiology: Diagnostic Principles and Practice
https://books.google.com/books?id=qEXzDwAAQBAJ&pg=PA8&lpg=PA8&dq=dark+quencher+pcr&source=bl&ots=mJ8O8Xqlvy&sig=ACfU3U3kxRo9xi9Z9k3HH3zsSNFWncBoyw&hl=en&sa=X&ved=2ahUKEwi4wM7-rNr7AhW6STABHbnbCxUQ6AF6BQi-AhAD
Dark quencher probes are not degraded during PCR and can generate melting curves. Dark quencher probes (ELITech Group, Princeton, NJ) are available as ...
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80 Molecular Genetic Testing in Surgical Pathology
https://books.google.com/books?id=inGTPMSlNT0C&pg=PA94&lpg=PA94&dq=dark+quencher+pcr&source=bl&ots=daK8948AmL&sig=ACfU3U3NCyD3WmVlP2Qu7zAQYQ-yrnM1fQ&hl=en&sa=X&ved=2ahUKEwi4wM7-rNr7AhW6STABHbnbCxUQ6AF6BQjAAhAD
... IN Q - PCR Reporters : The chemical structures of FAM , TAMRA , DABCYL , and EDANS are shown in Figure 5.6 . bA so - called dark quencher . hv2 Quencher ...
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