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Google Keyword Rankings for : pgem t easy ligation protocol
1
T and pGEM®-T Easy Vector Systems - Promega Corporation
https://www.promega.com/-/media/files/resources/protocols/technical-manuals/0/pgem-t-and-pgem-t-easy-vector-systems-protocol.pdf
The pGEM®-T and pGEM®-T Easy Vector Systems include a 2X Rapid Ligation Buffer for ligation of PCR products. Reactions using this buffer may be incubated for 1 ...
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https://www.promega.com/-/media/files/resources/protocols/technical-manuals/0/pgem-t-and-pgem-t-easy-vector-systems-protocol.pdf
The pGEM®-T and pGEM®-T Easy Vector Systems include a 2X Rapid Ligation Buffer for ligation of PCR products. Reactions using this buffer may be incubated for 1 ...
→ Check Latest Keyword Rankings ←
2
T and pGEM®-T Easy Vector Systems
https://www.med.upenn.edu/robertsonlab/assets/user-content/documents/pGEM-T%20and%20pGEM-T%20Easy%20Vector%20Systems.pdf
The pGEM®-T and pGEM®-T Easy Vector Systems include a 2X Rapid Ligation. Buffer for ligation of PCR products. Reactions using this buffer may be incubated for 1 ...
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https://www.med.upenn.edu/robertsonlab/assets/user-content/documents/pGEM-T%20and%20pGEM-T%20Easy%20Vector%20Systems.pdf
The pGEM®-T and pGEM®-T Easy Vector Systems include a 2X Rapid Ligation. Buffer for ligation of PCR products. Reactions using this buffer may be incubated for 1 ...
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3
PCR Cloning with Blue/White Selection--CHEM 584
https://www.protocols.io/view/pcr-cloning-with-blue-white-selection-chem-584-bmtzk6p6.html
Use only the T4 DNA Ligase supplied with this system to perform pGEM®-T and pGEM®-T Easy Vector ligations. Other commercial preparations of T4 DNA ligase ...
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https://www.protocols.io/view/pcr-cloning-with-blue-white-selection-chem-584-bmtzk6p6.html
Use only the T4 DNA Ligase supplied with this system to perform pGEM®-T and pGEM®-T Easy Vector ligations. Other commercial preparations of T4 DNA ligase ...
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4
T and pGEM®-T Easy Vector Systems - Normale Sup
https://www.normalesup.org/~vorgogoz/protocols/manuals/pGEM-T.pdf
pGEM®-T Easy Vector (50ng/µl). •. 12µl Control Insert DNA (4ng/µl). •. 100u. T4 DNA Ligase. •. 200µl 2X Rapid Ligation Buffer, T4 DNA Ligase. •. 1. Protocol.
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https://www.normalesup.org/~vorgogoz/protocols/manuals/pGEM-T.pdf
pGEM®-T Easy Vector (50ng/µl). •. 12µl Control Insert DNA (4ng/µl). •. 100u. T4 DNA Ligase. •. 200µl 2X Rapid Ligation Buffer, T4 DNA Ligase. •. 1. Protocol.
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5
Cloning - Promega pGEM-T Easy kit
http://eebweb.arizona.edu/blast/cloning.pdf
Ligation. 1. Add to the reaction mixture: a. 3 µl Fresh PCR product b. 5 µl 2x Rapid Ligation Buffer c. 1 µl pGEM-T vector d. 1 µl T4 DNA Ligase.
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http://eebweb.arizona.edu/blast/cloning.pdf
Ligation. 1. Add to the reaction mixture: a. 3 µl Fresh PCR product b. 5 µl 2x Rapid Ligation Buffer c. 1 µl pGEM-T vector d. 1 µl T4 DNA Ligase.
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6
pGEM T easy vector for cloning - Molecular Biology
http://www.protocol-online.org/biology-forums/posts/20319.html
added 3ul of the purified pcr product for ligation reaction. Added T4 DNA ligase and the ligase buffer provided along with the kit. In the kit ...
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http://www.protocol-online.org/biology-forums/posts/20319.html
added 3ul of the purified pcr product for ligation reaction. Added T4 DNA ligase and the ligase buffer provided along with the kit. In the kit ...
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7
Pgem T Easy Vector Protocol - Always Pure Organics
https://www.alwayspureorganics.com/wp-content/uploads/formidable/6/pgem-t-easy-vector-protocol.pdf
around grab the PGEM-T Easy Vector and T4 DNA ligase. What diseases can PCR detect? Setting up a protocol online pcr is the pgem t easy vector protocol the ...
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https://www.alwayspureorganics.com/wp-content/uploads/formidable/6/pgem-t-easy-vector-protocol.pdf
around grab the PGEM-T Easy Vector and T4 DNA ligase. What diseases can PCR detect? Setting up a protocol online pcr is the pgem t easy vector protocol the ...
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8
Transformation-of-pGEM-T-Easy-vector.pdf
https://haveylab.horticulture.wisc.edu/wp-content/uploads/sites/66/2016/05/Transformation-of-pGEM-T-Easy-vector.pdf
Transformation protocol when using the pGEM-T and pGEM-T Easy Vector. Ligation Reactions. 1. Prepare 1 LB/ampicillin//X-Gal (LBA plates are 1ml of 100mM Amp ...
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https://haveylab.horticulture.wisc.edu/wp-content/uploads/sites/66/2016/05/Transformation-of-pGEM-T-Easy-vector.pdf
Transformation protocol when using the pGEM-T and pGEM-T Easy Vector. Ligation Reactions. 1. Prepare 1 LB/ampicillin//X-Gal (LBA plates are 1ml of 100mM Amp ...
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9
Standard Ligation Reaction | Stupar Lab
https://stuparlab.cfans.umn.edu/protocols/standard-ligation-reaction
This protocol describes a standard ligation reaction for cloning a PCR fragment using Promega's pGEM-T Easy Vector system. ... Thaw competent cells (TOP10 cells ...
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https://stuparlab.cfans.umn.edu/protocols/standard-ligation-reaction
This protocol describes a standard ligation reaction for cloning a PCR fragment using Promega's pGEM-T Easy Vector system. ... Thaw competent cells (TOP10 cells ...
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10
pgem-t and pgem-t easy vector systems protocol.pdf
https://www.coursehero.com/file/37110932/pgem-t-and-pgem-t-easy-vector-systems-protocolpdf/
2.Set up ligation reactions as described below.Note:Use 0.5ml tubes known to have low DNA-binding capacity (e.g., VWR Cat.# 20170-310).Vortex the 2X Rapid ...
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https://www.coursehero.com/file/37110932/pgem-t-and-pgem-t-easy-vector-systems-protocolpdf/
2.Set up ligation reactions as described below.Note:Use 0.5ml tubes known to have low DNA-binding capacity (e.g., VWR Cat.# 20170-310).Vortex the 2X Rapid ...
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11
A Versatile Zero Background T-Vector System for Gene ... - NCBI
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705043/
Ligations using Promega T4 DNA ligase, the same product used by the pGEM-T easy vector system, consistently gave very high cloning efficiency ...
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705043/
Ligations using Promega T4 DNA ligase, the same product used by the pGEM-T easy vector system, consistently gave very high cloning efficiency ...
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12
pGEM-T Vector Systems Video - YouTube
https://www.youtube.com/watch?v=Mab5sUDwDHk
Promega Corporation
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https://www.youtube.com/watch?v=Mab5sUDwDHk
Promega Corporation
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13
pGEM-T Easy Vector Systems From Promega - Biocompare
https://www.biocompare.com/Product-Reviews/40766-pGEM-T-Easy-Vector-Systems-From-Promega/
The protocol also contains useful details about the plasmid design and restriction sites. The experimental procedure itself involves a DNA ...
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https://www.biocompare.com/Product-Reviews/40766-pGEM-T-Easy-Vector-Systems-From-Promega/
The protocol also contains useful details about the plasmid design and restriction sites. The experimental procedure itself involves a DNA ...
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14
Can someone help with a problem in pGEM T vector ligation ...
https://www.researchgate.net/post/Can_someone_help_with_a_problem_in_pGEM_T_vector_ligation_transformation
Problem may be the gene of interest you eluted may be having impurities like salts preventing the ligation to occur. pGEMT-Easy vector is highly efficient ...
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https://www.researchgate.net/post/Can_someone_help_with_a_problem_in_pGEM_T_vector_ligation_transformation
Problem may be the gene of interest you eluted may be having impurities like salts preventing the ligation to occur. pGEMT-Easy vector is highly efficient ...
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15
Promega pGEM™-T and pGEM™-T Easy Vector Systems
https://www.fishersci.com/shop/products/promega-pgem-t-pgem-t-easy-vector-systems-4/p-127641
Includes: Vector, Control insert DNA, T4 DNA ligase, Ligation buffer, High efficiency competent cells (Mfr. Nos. A3610 and A1380 only).
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https://www.fishersci.com/shop/products/promega-pgem-t-pgem-t-easy-vector-systems-4/p-127641
Includes: Vector, Control insert DNA, T4 DNA ligase, Ligation buffer, High efficiency competent cells (Mfr. Nos. A3610 and A1380 only).
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16
Ligation Protocol with T4 DNA Ligase (M0202) - NEB
https://www.neb.com/protocols/0001/01/01/dna-ligation-with-t4-dna-ligase-m0202
Protocol · Set up the following reaction in a microcentrifuge tube on ice. · Gently mix the reaction by pipetting up and down and microfuge briefly. · For cohesive ...
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https://www.neb.com/protocols/0001/01/01/dna-ligation-with-t4-dna-ligase-m0202
Protocol · Set up the following reaction in a microcentrifuge tube on ice. · Gently mix the reaction by pipetting up and down and microfuge briefly. · For cohesive ...
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17
Solved 53 Protocol 11 - Ligation of PCR product to PGEM-T
https://www.chegg.com/homework-help/questions-and-answers/ligation-pcr-product-pgem-t-easy-plasmid-first-two-pics-referance-need-help-hw-question-q60746120
coli with plasmid that hopefully contains the PCR product inside if it. REQUIRED MATERIALS PGEM-T Easy plasmid (Kit ordered from Fisher PR-1380) 2x rapid ...
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https://www.chegg.com/homework-help/questions-and-answers/ligation-pcr-product-pgem-t-easy-plasmid-first-two-pics-referance-need-help-hw-question-q60746120
coli with plasmid that hopefully contains the PCR product inside if it. REQUIRED MATERIALS PGEM-T Easy plasmid (Kit ordered from Fisher PR-1380) 2x rapid ...
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18
pGEM-T Easy Sequence and Map - SnapGene
https://www.snapgene.com/resources/plasmid-files/?set=basic_cloning_vectors&plasmid=pGEM-T_Easy
pGEM-T Easy. Parental vector for TA cloning of PCR products. The insertion site is flanked by BstZI, EcoRI, and NotI sites. Sequence Author: Promega.
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https://www.snapgene.com/resources/plasmid-files/?set=basic_cloning_vectors&plasmid=pGEM-T_Easy
pGEM-T Easy. Parental vector for TA cloning of PCR products. The insertion site is flanked by BstZI, EcoRI, and NotI sites. Sequence Author: Promega.
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19
PCR Subcloning - Amazon S3
https://s3-us-west-2.amazonaws.com/oww-files-public/a/a4/Pcr_subcloning_row.pdf
A method of choice for cloning PCR products is T-Vector cloning. ... pGEM-T Easy ligation reactions were set up at room temperature (24°C) and allowed to.
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https://s3-us-west-2.amazonaws.com/oww-files-public/a/a4/Pcr_subcloning_row.pdf
A method of choice for cloning PCR products is T-Vector cloning. ... pGEM-T Easy ligation reactions were set up at room temperature (24°C) and allowed to.
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20
TA Cloning - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/ta-cloning
Compatible TA cloning vectors with T overhangs are supplied by different ... and then the fragment was cloned into pGEM-T Easy vector by TA-cloning as ...
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https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/ta-cloning
Compatible TA cloning vectors with T overhangs are supplied by different ... and then the fragment was cloned into pGEM-T Easy vector by TA-cloning as ...
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21
Standard DNA Cloning - Bio-protocol
https://bio-protocol.org/pdf/bio-protocol52.pdf
Or, first clone PCR product to pGEM T-easy vector, and then generate insert DNA from the resulting plasmid. d. Perform gel purification of insert DNA. e.
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https://bio-protocol.org/pdf/bio-protocol52.pdf
Or, first clone PCR product to pGEM T-easy vector, and then generate insert DNA from the resulting plasmid. d. Perform gel purification of insert DNA. e.
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22
A novel series of high-efficiency vectors for TA cloning and ...
https://www.nature.com/articles/s41598-019-42868-6
This direct PCR cloning protocol yielded colony-formation rates and ... As a control for commercially available T-vector, pGEM-T Easy was ...
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https://www.nature.com/articles/s41598-019-42868-6
This direct PCR cloning protocol yielded colony-formation rates and ... As a control for commercially available T-vector, pGEM-T Easy was ...
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23
PCR Product Cloning - iGEM
https://static.igem.org/mediawiki/2014/2/23/PCR_Product_Clonig_2.pdf
Blunt-end Cloning – PUC19/ SmaI ... ligation in E. coli DH5α. (see the Transformation in E. coli Protocol) ... pGEM-T-Easy Cloning - Promega.
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https://static.igem.org/mediawiki/2014/2/23/PCR_Product_Clonig_2.pdf
Blunt-end Cloning – PUC19/ SmaI ... ligation in E. coli DH5α. (see the Transformation in E. coli Protocol) ... pGEM-T-Easy Cloning - Promega.
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24
cloning in escherichia coli
https://www.lehigh.edu/~mrk5/bios116-cloning%20in%20E.pdf
chain reaction with a bacterial plasmid called pGEM-T®. Next, you will introduce the products of this ligation reaction into E. coli cells in a standard ...
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https://www.lehigh.edu/~mrk5/bios116-cloning%20in%20E.pdf
chain reaction with a bacterial plasmid called pGEM-T®. Next, you will introduce the products of this ligation reaction into E. coli cells in a standard ...
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25
pGEM(R)-T Easy Vector System I ยี่ห้อ Promega (Stroge. Temp
https://www.npchem.co.th/product/A1360
The pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the insertion site to provide a compatible overhang for PCR products. The provided 2X Rapid ...
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https://www.npchem.co.th/product/A1360
The pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the insertion site to provide a compatible overhang for PCR products. The provided 2X Rapid ...
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26
TOPO™ TA Cloning™ Kit for Subcloning, without competent ...
https://www.thermofisher.com/order/catalog/product/450641
The pCR™ 2.1-TOPO™ TA vector is linearized with 3'-thymidine (T) overhangs for direct ligation of Taq polymerase–amplified PCR products (TA cloning™) and is ...
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https://www.thermofisher.com/order/catalog/product/450641
The pCR™ 2.1-TOPO™ TA vector is linearized with 3'-thymidine (T) overhangs for direct ligation of Taq polymerase–amplified PCR products (TA cloning™) and is ...
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27
Selecting Correctly Expressing Recombinants - Sigma-Aldrich
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/cloning-and-expression/restriction-enzyme-cloning-manual-recombinants
Protocol · Start with fresh overnight liquid cultures (A single colony picked and inoculated into 3mL LB medium the evening before). · Add approximately 1.5 mL of ...
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https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/cloning-and-expression/restriction-enzyme-cloning-manual-recombinants
Protocol · Start with fresh overnight liquid cultures (A single colony picked and inoculated into 3mL LB medium the evening before). · Add approximately 1.5 mL of ...
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28
FastCloning: a highly simplified, purification-free, sequence ...
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-92
FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method · 75k Accesses · 258 Citations · 144 ...
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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-92
FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method · 75k Accesses · 258 Citations · 144 ...
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29
pGEM®-T Easy Vector Systems, Promega® | VWR
https://us.vwr.com/store/product/4762668/pgem-t-easy-vector-systems-promega
Convenience and Flexibility for Cloning PCR Products · Three options for removing the insert with a single digest (BstZI, EcoRI or NotI ) · Ligation can be ...
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https://us.vwr.com/store/product/4762668/pgem-t-easy-vector-systems-promega
Convenience and Flexibility for Cloning PCR Products · Three options for removing the insert with a single digest (BstZI, EcoRI or NotI ) · Ligation can be ...
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30
pGEM®-T Easy Vector Systems from Promega Corp.
https://www.selectscience.net/products/pgem-t-easy-vector-systems/?prodID=85074
It has high efficiency for the genes we work with and purchase the system with the competent cells as we are guaranteed results. The step by step protocol is ...
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https://www.selectscience.net/products/pgem-t-easy-vector-systems/?prodID=85074
It has high efficiency for the genes we work with and purchase the system with the competent cells as we are guaranteed results. The step by step protocol is ...
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31
TA cloning: Ultimate guide - Sharebiology
https://sharebiology.com/ta-cloning-ultimate-guide/
TA cloning is a simple method to clone any desirable fragment with an extra A (Adenine nucleotide) overhang into any linearized vector with T (Thymidine ...
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https://sharebiology.com/ta-cloning-ultimate-guide/
TA cloning is a simple method to clone any desirable fragment with an extra A (Adenine nucleotide) overhang into any linearized vector with T (Thymidine ...
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32
Making Taq DNA polymerase in the undergraduate - jstor
https://www.jstor.org/stable/4608798
ligate agarose gel-purified amplicons into. pGEM T-easy plasmid following the Promega li- gation protocol. The completed ligation reactions, involving.
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https://www.jstor.org/stable/4608798
ligate agarose gel-purified amplicons into. pGEM T-easy plasmid following the Promega li- gation protocol. The completed ligation reactions, involving.
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33
Riboprobe synthesis for in situ hybridization Martindale Lab 1
https://www.whitney.ufl.edu/media/wwwwhitneyufledu/images/files/Riboprobe-synthesis-for-in-situ-hybridization.pdf
Isolate RNA using Trizol reagent protocol. ... pGEM-T plasmid ligation kit ... Ligase. 5ul. 2.5ul. 3.75ul. pGEM®-T or pGEM®-T Easy Vector.
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https://www.whitney.ufl.edu/media/wwwwhitneyufledu/images/files/Riboprobe-synthesis-for-in-situ-hybridization.pdf
Isolate RNA using Trizol reagent protocol. ... pGEM-T plasmid ligation kit ... Ligase. 5ul. 2.5ul. 3.75ul. pGEM®-T or pGEM®-T Easy Vector.
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34
T-Vectors pMD20 and pMD19 for PCR cloning - Takara Bio
https://www.takarabio.com/products/cloning/linkers-primers-and-cloning-vectors/cloning-vectors/t-vectors-pmd20-and-pmd19
pMD20 and pMD19 (Simple) T-Vectors are linearized PCR cloning vectors (cleaved by EcoRV) with single 3'-terminal thymidine residues (dT) at both ends.
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https://www.takarabio.com/products/cloning/linkers-primers-and-cloning-vectors/cloning-vectors/t-vectors-pmd20-and-pmd19
pMD20 and pMD19 (Simple) T-Vectors are linearized PCR cloning vectors (cleaved by EcoRV) with single 3'-terminal thymidine residues (dT) at both ends.
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35
Ligation-Convenience Kit Manual (4th edition)
https://www.nippongene.com/english/tds/tds_ligation-kit-e1201.pdf
Ligation reaction: 16°C, 5 min. Vector: pGEMR-T Easy; Promega Inc. (0.03 pmol). Insert: PCR product amplified by Gene Taq NT (Code No. 318-03231).
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https://www.nippongene.com/english/tds/tds_ligation-kit-e1201.pdf
Ligation reaction: 16°C, 5 min. Vector: pGEMR-T Easy; Promega Inc. (0.03 pmol). Insert: PCR product amplified by Gene Taq NT (Code No. 318-03231).
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36
QIAGEN PCR Cloning Kits
https://www.qiagen.com/us/products/discovery-and-translational-research/pcr-qpcr-dpcr/pcr-enzymes-and-kits/hifidelity-long-range-and-other-pcr/qiagen-pcr-cloning-kits/
Simply mix the PCR product directly with pDrive Cloning Vector and Ligation Master Mix, incubate, and then add the ligation reaction to competent cells for ...
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https://www.qiagen.com/us/products/discovery-and-translational-research/pcr-qpcr-dpcr/pcr-enzymes-and-kits/hifidelity-long-range-and-other-pcr/qiagen-pcr-cloning-kits/
Simply mix the PCR product directly with pDrive Cloning Vector and Ligation Master Mix, incubate, and then add the ligation reaction to competent cells for ...
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37
PCR Cloning Protocols - Springer Link
https://link.springer.com/content/pdf/10.1385/0896034836.pdf
well below the T^ as described above. When background problems are expected to be minimal, a simple two step SD PCR protocol can still be advantageous.
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https://link.springer.com/content/pdf/10.1385/0896034836.pdf
well below the T^ as described above. When background problems are expected to be minimal, a simple two step SD PCR protocol can still be advantageous.
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38
ligation protocol | ligation biology | ligation dna - GenScript
https://www.genscript.com/ligation-protocol.html
› ligation-protocol
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https://www.genscript.com/ligation-protocol.html
› ligation-protocol
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39
TA cloning - Wikipedia
https://en.wikipedia.org/wiki/TA_cloning
TA cloning (also known as rapid cloning or T cloning) is a subcloning technique that avoids the use of restriction enzymes and is easier and quicker than ...
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https://en.wikipedia.org/wiki/TA_cloning
TA cloning (also known as rapid cloning or T cloning) is a subcloning technique that avoids the use of restriction enzymes and is easier and quicker than ...
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40
Parasite Genomics Protocols - Page 162 - Google Books Result
https://books.google.com/books?id=v49nSS1I0joC&pg=PA162&lpg=PA162&dq=pgem+t+easy+ligation+protocol&source=bl&ots=_KJD28aNCm&sig=ACfU3U3zgaHlzemfTc0ypDKNAxg3g4qcQA&hl=en&sa=X&ved=2ahUKEwjTxMrl9eL7AhVtmHIEHZrzCxsQ6AF6BQjKARAD
Ligate the eluted DNA band to the pGEM-T-EASY vector using the following protocol, and mix: T4 DNA Ligase 10X buffer 1 μL pGEM-T-EASY vector, ...
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https://books.google.com/books?id=v49nSS1I0joC&pg=PA162&lpg=PA162&dq=pgem+t+easy+ligation+protocol&source=bl&ots=_KJD28aNCm&sig=ACfU3U3zgaHlzemfTc0ypDKNAxg3g4qcQA&hl=en&sa=X&ved=2ahUKEwjTxMrl9eL7AhVtmHIEHZrzCxsQ6AF6BQjKARAD
Ligate the eluted DNA band to the pGEM-T-EASY vector using the following protocol, and mix: T4 DNA Ligase 10X buffer 1 μL pGEM-T-EASY vector, ...
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41
Straightforward procedure for laboratory production of DNA ...
https://europepmc.org/article/med/22496965
This protocol is a combination of routinely employed methods: cloning, PCR, and partial digestion with restriction enzymes. DNA fragments of 100 bp with ...
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https://europepmc.org/article/med/22496965
This protocol is a combination of routinely employed methods: cloning, PCR, and partial digestion with restriction enzymes. DNA fragments of 100 bp with ...
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42
Cloning, Expression, and Purification of the rE2 Protein ...
https://www.heraldopenaccess.us/openaccess/cloning-expression-and-purification-of-the-re2-protein-human-papillomavirus-type-16
E2 gene amplification was performed by end-point PCR and later was cloned in the vector pGEM-t- easy in the E.Coli TOP 10F 'strain, the E2 amplicon was ...
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https://www.heraldopenaccess.us/openaccess/cloning-expression-and-purification-of-the-re2-protein-human-papillomavirus-type-16
E2 gene amplification was performed by end-point PCR and later was cloned in the vector pGEM-t- easy in the E.Coli TOP 10F 'strain, the E2 amplicon was ...
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43
Ambient Stable Quantitative PCR Reagents for the Detection ...
https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0000629
Although it is time-consuming and laborious, this protocol is still a ... from the 91001 genomic DNA for cloning into the pGEM-T Easy Vector ...
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https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0000629
Although it is time-consuming and laborious, this protocol is still a ... from the 91001 genomic DNA for cloning into the pGEM-T Easy Vector ...
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44
Nucleic Acid Amplification Protocols and Applications Guide
https://fire.biol.wwu.edu/cmoyer/zztemp_fire/biol324_W08/pcr_guide.pdf
Example of an RT-PCR Protocol ... The polymerase chain reaction (PCR) is a relatively simple ... Researchers used the pGEM®-T Vector System to clone the.
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https://fire.biol.wwu.edu/cmoyer/zztemp_fire/biol324_W08/pcr_guide.pdf
Example of an RT-PCR Protocol ... The polymerase chain reaction (PCR) is a relatively simple ... Researchers used the pGEM®-T Vector System to clone the.
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45
Cloning Promega - B. Restriction Digest of PCR Product
https://www.studocu.com/vn/document/dai-hoc-nong-lam-thanh-pho-ho-chi-minh/tieng-anh-chuyen-nganh/cloning-promega-tieng-anh-chuyen-nganh-cong-nghe-sinh-hoc/22322898
The pGEM®-T and pGEM®-T Easy Vectors also contain the origin of replication of filamentous phage f1 for the preparation of single-stranded DNA (ssDNA). Both ...
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https://www.studocu.com/vn/document/dai-hoc-nong-lam-thanh-pho-ho-chi-minh/tieng-anh-chuyen-nganh/cloning-promega-tieng-anh-chuyen-nganh-cong-nghe-sinh-hoc/22322898
The pGEM®-T and pGEM®-T Easy Vectors also contain the origin of replication of filamentous phage f1 for the preparation of single-stranded DNA (ssDNA). Both ...
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46
Genetics Lab 4: Ligation and Transformation - Quizlet
https://quizlet.com/233561693/genetics-lab-4-ligation-and-transformation-flash-cards/
T-A cloning allows you to directly ligate a PCR product into the pGEM-t vector, ... the vector (pGEMt-easy) also includes oriC, an origin of replication.
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https://quizlet.com/233561693/genetics-lab-4-ligation-and-transformation-flash-cards/
T-A cloning allows you to directly ligate a PCR product into the pGEM-t vector, ... the vector (pGEMt-easy) also includes oriC, an origin of replication.
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47
Manual: pBluescript II Phagemid Vectors
https://www.chem-agilent.com/pdf/strata/212205.pdf
9 The following protocol is recommended: 1. Phosphorylation of the oligonucleotide with polynucleotide kinase: 100 ng of oligonucleotide. 4 μl of 10× ligase ...
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https://www.chem-agilent.com/pdf/strata/212205.pdf
9 The following protocol is recommended: 1. Phosphorylation of the oligonucleotide with polynucleotide kinase: 100 ng of oligonucleotide. 4 μl of 10× ligase ...
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48
Lab 7: pAMP & pKAN ligation
https://www.csus.edu/indiv/r/rogersa/bio181/lab7.pdf
Cut the insulin gene out of chromosome 11 using HindIII. Purify this fragment. Open the plasmid pGEM at its multicloning site using HindIII. Mix and ligate; ...
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https://www.csus.edu/indiv/r/rogersa/bio181/lab7.pdf
Cut the insulin gene out of chromosome 11 using HindIII. Purify this fragment. Open the plasmid pGEM at its multicloning site using HindIII. Mix and ligate; ...
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49
Manual for Soil Analysis - Monitoring and Assessing Soil ...
https://books.google.com/books?id=RBFD0BfWKBUC&pg=PA216&lpg=PA216&dq=pgem+t+easy+ligation+protocol&source=bl&ots=jf-1slmtkH&sig=ACfU3U1Oq-SmzrXUKROt2iuTVSDIp53NBQ&hl=en&sa=X&ved=2ahUKEwjTxMrl9eL7AhVtmHIEHZrzCxsQ6AF6BQjJARAD
A ligation reaction is set up as described in the pGEM-T Easy Vector ... ratio) kb size ofvector (3.0kb for pGEM-T Easy) Transformation protocols of E. coli ...
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https://books.google.com/books?id=RBFD0BfWKBUC&pg=PA216&lpg=PA216&dq=pgem+t+easy+ligation+protocol&source=bl&ots=jf-1slmtkH&sig=ACfU3U1Oq-SmzrXUKROt2iuTVSDIp53NBQ&hl=en&sa=X&ved=2ahUKEwjTxMrl9eL7AhVtmHIEHZrzCxsQ6AF6BQjJARAD
A ligation reaction is set up as described in the pGEM-T Easy Vector ... ratio) kb size ofvector (3.0kb for pGEM-T Easy) Transformation protocols of E. coli ...
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50
Ribozymes and siRNA protocols - Page 284 - Google Books Result
https://books.google.com/books?id=lz0xJZEs-KQC&pg=PA284&lpg=PA284&dq=pgem+t+easy+ligation+protocol&source=bl&ots=tZ3hc1T06l&sig=ACfU3U1iokwpO4kQRxbKhLw8-kKkVuGiHA&hl=en&sa=X&ved=2ahUKEwjTxMrl9eL7AhVtmHIEHZrzCxsQ6AF6BQjGARAD
... and cloned into a T-tailed vector, pGEM-T-Easy (Promega Biotech, WI) to yield plasmid pGEM- gag. For ligation of the insert into pGEM-T-vector system, ...
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https://books.google.com/books?id=lz0xJZEs-KQC&pg=PA284&lpg=PA284&dq=pgem+t+easy+ligation+protocol&source=bl&ots=tZ3hc1T06l&sig=ACfU3U1iokwpO4kQRxbKhLw8-kKkVuGiHA&hl=en&sa=X&ved=2ahUKEwjTxMrl9eL7AhVtmHIEHZrzCxsQ6AF6BQjGARAD
... and cloned into a T-tailed vector, pGEM-T-Easy (Promega Biotech, WI) to yield plasmid pGEM- gag. For ligation of the insert into pGEM-T-vector system, ...
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51
Sequence-Specific DNA Binders for the Therapy of ...
https://books.google.com/books?id=lplXEAAAQBAJ&pg=PA56&lpg=PA56&dq=pgem+t+easy+ligation+protocol&source=bl&ots=gT2-W2jHGe&sig=ACfU3U3_LGGsyA4AZgCBQEMlO88GFBBN6g&hl=en&sa=X&ved=2ahUKEwjTxMrl9eL7AhVtmHIEHZrzCxsQ6AF6BQjHARAD
... was ligated with pGEM®-T Easy Vector (Promega) using Ligation high Ver. ... and ligation reaction were performed following the manufacture's protocol.
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https://books.google.com/books?id=lplXEAAAQBAJ&pg=PA56&lpg=PA56&dq=pgem+t+easy+ligation+protocol&source=bl&ots=gT2-W2jHGe&sig=ACfU3U3_LGGsyA4AZgCBQEMlO88GFBBN6g&hl=en&sa=X&ved=2ahUKEwjTxMrl9eL7AhVtmHIEHZrzCxsQ6AF6BQjHARAD
... was ligated with pGEM®-T Easy Vector (Promega) using Ligation high Ver. ... and ligation reaction were performed following the manufacture's protocol.
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52
pGEM -T and pGEM -T Easy Vector Systems - Promega/media/Files ...
https://cupdf.com/document/pgem-t-and-pgem-t-easy-vector-systems-mediafilesresourcesprotocolstechnical.html
Protocol for Ligations Using the pGEM-T and pGEM-T Easy Vectors and the 2X Rapid Ligation Buffer ..43.A. Ligation Protocol .
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https://cupdf.com/document/pgem-t-and-pgem-t-easy-vector-systems-mediafilesresourcesprotocolstechnical.html
Protocol for Ligations Using the pGEM-T and pGEM-T Easy Vectors and the 2X Rapid Ligation Buffer ..43.A. Ligation Protocol .
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53
encodes resistance to ampicillin - Top Limousine NYC
https://toplimousinenyc.com/tnv/encodes-resistance-to-ampicillin
The amplified fragment was cloned into pGEM-T Easy (Promega) forming pGEM-ITR. ... The same protocol (except that the cells were grown for 20 hours instead ...
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https://toplimousinenyc.com/tnv/encodes-resistance-to-ampicillin
The amplified fragment was cloned into pGEM-T Easy (Promega) forming pGEM-ITR. ... The same protocol (except that the cells were grown for 20 hours instead ...
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